It officially opened on September 24, 1903 with a student production of The Birds by Aristophanes. However, while still under construction in May 1903, the theatre hosted a graduation ceremony with an address by President Theodore Roosevelt, who was a friend of Wheeler's from New York.
Over the years, the Greek Theatre has seen performances from hundreds of musical and theatrical artists. The Greek has also long been the venue for a number of annual UC Berkeley events including departmental graduation ceremonies, the Commencement Convocation for graduating seniors, and the Big Game Bonfire Rally before the Big Game each year with Stanford. Charter Day ceremonies and inaugurations of University of California presidents and Berkeley chancellors have been held in the Greek Theatre. Berkeley High school also uses the Greek Theatre for their graduation ceremonies.
Another Planet Entertainment became the exclusive concert promoter at the Greek in 2004.