Heat stabilization

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This article is about a biochemistry technique. For wine-making process, see Winemaking#Cold and heat stabilization.

Heat stabilization is an additive-free preservation technology for tissue samples which stops degradation and changes immediately and permanently. Heat stabilization uses rapid conductive heating, under controlled pressure, to generate a fast, homogenous and irreversible thermal denaturation of proteins, resulting in a complete and permanent elimination of all enzymatic activity that would otherwise cause further biological changes to the tissue sample ex vivo. Due to the permanent inactivation of enzymes, heat stabilization overcomes the drawbacks of conventional tissue sample preservation techniques, such as snap-freezing followed by inhibitors.[1]


Understanding the role of proteins, peptides and small molecules in normal and diseased tissue is crucial to defining their potential use as drugs, drug targets or disease biomarkers. Yet biological changes begin the moment tissue is removed from its native environment. Dramatic alterations at the molecular level occur within seconds e.g. changed metabolism, catabolic fragmentation of large molecules (such as ATP) occurs in order to release energy, leading to disrupted control mechanisms, phosphorylation states are altered and proteins begin to degrade. As a consequence vital information may be lost or distorted, leading to inter-sample variation, risk of incorrect data interpretation and potentially misleading conclusions.[2]

Heat stabilization offers significant advantages over conventional approaches to preventing biological change.[3] It can be used to replace snap freezing followed by inhibitors, pH changes, organic solvents or cross-linking. It can also be used with frozen tissue, allowing stabilization of stored samples.

Heat stabilization can be used for almost any kind of tissue sample, and has been verified to be compatible with many down­stream analytical techniques such as mass spectrometry,[4] phospho-shotgun,[5] MALDI imaging,[6] Western blot,[7] 1D and 2D gels, reversed-phased protein arrays,[8] RIA and ELISA.

References[edit]

  1. ^ Heat Stabilization of the Tissue Proteome: A New Technology for Improved Proteomics, M. Svensson et al. J. Proteome Res., 2009, 8 (2), pp 974-981
  2. ^ The impact of biosampling procedures on molecular data interpretation. Scholz et al., Molecular & cellular proteomics. February 2013
  3. ^ Eliminating biological change after excision. M Söderquist., GEN Jan. 2013 Tutorial
  4. ^ Thermal stabilization of tissues and the preservation of protein phosphorylation states for two-dimensional gel electrophoresis. Smejkal et al., Electrophoresis 2011, Volume 32, pages 2206–2215
  5. ^ Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Lundby et al., Nature Communications. June 2012
  6. ^ Localisation of adenine nucleotides in heat-stabilised mouse brains using ion mobility enabled MALDI Imaging. Blatherwick et al., International Journal of Mass Spectrometry. February 2013.
  7. ^ Expression of trisomic proteins in Down syndrome model systems. Spellman et al., Gene. October 2012
  8. ^ Protein profiles in Tc1 mice implicate novel pathway perturbations in the Down syndrome brain. Ahmed et al., Human Molecular Genetics. January 2013