|Classification and external resources|
A schematic drawing of platelet factor 4, which when bound to heparin leads to an immune response in HIT.
Heparin-induced thrombocytopenia (HIT) is the development of thrombocytopenia (a low platelet count), due to the administration of various forms of heparin, an anticoagulant. HIT predisposes to thrombosis, the abnormal formation of blood clots inside a blood vessel, and when thrombosis is identified the condition is called heparin-induced thrombocytopenia and thrombosis (HITT). HIT is caused by the formation of abnormal antibodies that activate platelets. If someone receiving heparin develops new or worsening thrombosis, or if the platelet count falls, HIT can be confirmed with specific blood tests.
The treatment of HIT requires both protection from thrombosis and choice of an agent that will not reduce the platelet count further. Several agents exist for this purpose, mainly lepirudin and argatroban. While heparin was discovered in the 1930s, HIT was not reported until the 1960s and 1970s.
Signs and symptoms
Heparin may be used for both prevention and the treatment of thrombosis. It exists in two main forms: an "unfractionated" form that can be injected under the skin or through an intravenous infusion, and a "low molecular weight" form that is generally given subcutaneously (administered under the skin). Commonly used low molecular weight heparins are enoxaparin, dalteparin, and tinzaparin.
In HIT, the platelet count in the blood falls below the normal range, a condition called thrombocytopenia. However, it is generally not low enough to lead to an increased risk of bleeding. Most people with HIT will therefore not experience any symptoms. Typically the platelet count will fall 5–14 days after heparin is first given; if someone has received heparin in the previous three months, the fall in platelet count may occur sooner, sometimes within a day.
The most common symptom of HIT is enlargement or extension of a previously diagnosed blood clot, or the development of a new blood clot elsewhere in the body. This may take the form of clots either in arteries or veins, causing arterial or venous thrombosis, respectively. Examples of arterial thrombosis are stroke, myocardial infarction ("heart attack"), and acute leg ischemia. Venous thrombosis may occur in the leg or arm in the form of deep vein thrombosis (DVT) and in the lung in the form of a pulmonary embolism (PE); the latter usually originate in the leg but migrate to the lung.
In those receiving heparin through an intravenous infusion, a complex of symptoms ("systemic reaction") may occur when the infusion is started. These include fever, chills, high blood pressure, a fast heart rate, shortness of breath, and chest pain. This happens in about a quarter of people with HIT. Others may develop a skin rash consisting of red spots.
Heparin occurs naturally in the human body, but the development of HIT antibodies suggests heparin sulfate may act as a hapten, and thus be targeted by the immune system. In HIT, the immune system forms antibodies against heparin when it is bound to a protein called platelet factor 4 (PF4). These antibodies are usually of the IgG class and their development usually takes about five days. However, those who have been exposed to heparin in the last few months may still have circulating IgG, as IgG-type antibodies generally continue to be produced even when their precipitant has been removed. This is similar to immunity against certain microorganisms, with the difference that the HIT antibody does not persist more than three months. HIT antibodies have been found in individuals with thrombocytopenia and thrombosis who had no prior exposure to heparin sulfate, but the majority are found in people who are receiving heparin.
The IgG antibodies form a complex with heparin and PF4 in the bloodstream. The tail of the antibody then binds to the FcγIIa receptor, a protein on the surface of the platelet. This results in platelet activation and the formation of platelet microparticles, which initiate the formation of blood clots; the platelet count falls as a result, leading to thrombocytopenia.
Formation of PF4-heparin antibodies is common in people receiving heparin, but only a proportion of these develop thrombocytopenia or thrombosis. This has been referred to as an "iceberg phenomenon".
HIT may be suspected if blood tests show a falling platelet count in someone receiving heparin, even if the heparin has already been discontinued. Professional guidelines recommend that people receiving heparin have a complete blood count (which includes a platelet count) on a regular basis while receiving heparin.
However, not all people with a falling platelet count while receiving heparin turn out to have HIT. The timing, severity of the thrombocytopenia, the occurrence of new thrombosis, and the presence of alternative explanations, all determine the likelihood that HIT is present. A commonly used score to predict the likelihood of HIT is the "4 Ts" score introduced in 2003. A score of 0–8 points is generated; if the score is 0-3, HIT is unlikely. A score of 4–5 indicates intermediate probability, while a score of 6–8 makes it highly likely. Those with a high score may need to be treated with an alternative drug while more sensitive and specific tests for HIT are performed, while those with a low score can safely continue receiving heparin as the likelihood that they have HIT is extremely low. In an analysis of the reliability of the 4T score, a low score had a negative predictive value of 0.998, while an intermediate score had a positive predictive value of 0.14 and a high score a positive predictive value of 0.64; intermediate and high scores therefore warrant further investigation.
|Element||The 4T score for heparin-induced thrombocytopenia|
|Thrombocytopenia||2 points if the fall in platelet count is >50% of the previous value, or the lowest count (nadir) is 20–100 × 109/liter
1 point if the fall is 30–50% or the nadir is 10–19 × 109/liter
No points if the fall is less than 30% or the nadir is <10 × 109/liter.
|Timing||2 points if the fall is between days 5–10 after commencement of treatment
1 point if the fall is after day 10. If someone has been exposed to heparin within the last 30 days and then has a drop in platelet count within a day of reexposure, 2 points are given. If the previous exposure was 30–100 days ago, 1 point
If the fall is early but there has been no previous heparin exposure, no points.
|Thrombosis||2 points in new proven thrombosis, skin necrosis (see below), or systemic reaction
1 point if progressive or recurrent thrombosis, silent thrombosis or red skin lesions
No points if there are no symptoms.
|Alternative cause possible||2 points if no other cause
1 point if there is a possible alternative cause
No points if there is a definite alternative cause.
A less used, but equally as effective, noninvasive pretest method for ruling out HIT is the HIT Expert Probability (HEP) score, which was developed based on expert opinion by having HIT diagnosticians agree upon a weighted scoring system using different clinical findings. The scoring includes factors like a patient's platelet nadir, severe infection, skin necrosis at heparin injection site, and severe overactive clotting lab values. Each of these findings are weighted upon their statistical correlation with heparin-induced thrombocytopenia as agreed upon by the panel of experts using a scale from -2 to 3 - the more negative the less correlated with HIT, the more positive, the more correlated with HIT.
The first screening test in someone suspected of having HIT is aimed at detecting antibodies against heparin-PF4 complexes. This may be with a laboratory test of the ELISA (enzyme-linked immunosorbent assay) type. The ELISA test, however, detects all circulating antibodies that bind heparin-PF4 complexes, and may also falsely identify antibodies that do not cause HIT. Therefore, those with a positive ELISA are tested further with a functional assay. This test uses platelets and serum from the patient; the platelets are washed and mixed with serum and heparin. The sample is then tested for the release of serotonin, a marker of platelet activation. If this serotonin release assay (SRA) shows high serotonin release, the diagnosis of HIT is confirmed. The SRA test is difficult to perform and is usually only done in regional laboratories.
Given the fact that HIT predisposes strongly to new episodes of thrombosis, it is not sufficient to simply discontinue the heparin administration. Generally, an alternative anticoagulant is needed to suppress the thrombotic tendency while the generation of antibodies stops and the platelet count recovers. To make matters more complicated, the other most commonly used anticoagulant, warfarin, should not be used in HIT until the platelet count is at least 150 x 10^9/L because there is a very high risk of warfarin necrosis in people with HIT who have low platelet counts. Warfarin necrosis is the development of skin gangrene in those receiving warfarin or a similar vitamin K inhibitor. If the patient was receiving warfarin at the time when HIT is diagnosed, the activity of warfarin is reversed with vitamin K. Transfusing platelets is discouraged, as there is a theoretical risk that this may worsen the risk of thrombosis; the platelet count is rarely low enough to be the principal cause of significant hemorrhage.
Three agents are used to provide anticoagulation in patients with strongly suspected or proven HIT: danaparoid, lepirudin, and argatroban. These are alternatives to heparin therapy. Not all agents are registered in all countries. For instance, argatroban is only recently licensed in the United Kingdom, and danaparoid is not available in the United States. Lepirudin production stopped on May 31, 2012. A fourth agent acting as a Factor Xa inhibitor also has an off label use for HIT treatment known as fondaparinux and is available and commonly used in the United States.
According to systematic review, patients treated with lepirudin for HIT showed a relative risk reduction of clinical outcome (death, amputation, etc.) to be 0.52 and 0.42 when compared to patient controls. In addition, patients treated with argatroban for HIT showed a relative risk reduction of the above clinical outcomes to be 0.20 and 0.18.
The exact number of cases of HIT in the general population is unknown. What is known is that women receiving heparin after a recent surgical procedure, particularly cardiothoracic surgery, have a higher risk, while the risk is very low in women just before and after giving birth. Some studies have shown that HIT is less common in those receiving low molecular weight heparin.
While heparin was introduced for clinical use in the late 1930s, new thrombosis in people treated with heparin was not described until 1957, when vascular surgeons reported the association. The fact that this phenomenon occurred together with thrombocytopenia was reported in 1969; prior to this time, platelet counts were not routinely performed. A 1973 report established HIT as a diagnosis, as well as suggesting that its features were the result of an immune process.
Initially, various theories existed about the exact cause of the low platelets in HIT. Gradually, evidence accumulated on the exact underlying mechanism, and diagnostic tests for the condition emerged in the 1980s. Treatment was initially limited to aspirin and warfarin, but the 1990s saw the introduction of a number of agents that could provide anticoagulation without a risk of recurrent HIT. Older terminology distinguishes between two forms of heparin-induced thrombocytopenia: type 1 (mild, non-immune mediated and self-limiting fall in platelet count) and type 2, the form described above. Currently, the term HIT is used without a modifier to describe the immune-mediated severe form.
- Ahmed I, Majeed A, Powell R (September 2007). "Heparin induced thrombocytopenia: diagnosis and management update". Postgrad Med J 83 (983): 575–82. doi:10.1136/pgmj.2007.059188. PMC 2600013. PMID 17823223.
- Kelton JG, Warkentin TE (October 2008). "Heparin-induced thrombocytopenia: a historical perspective". Blood 112 (7): 2607–16. doi:10.1182/blood-2008-02-078014. PMID 18809774.
- Hirsh J, Bauer KA, Donati MB, Gould M, Samama MM, Weitz JI (June 2008). "Parenteral anticoagulants: American College of Chest Physicians Evidence-Based Clinical Practice Guidelines (8th Edition)". Chest 133 (6 Suppl): 141S–159S. doi:10.1378/chest.08-0689. PMID 18574264.
- Warkentin TE (2006). "Think of HIT". Hematology Am Soc Hematol Educ Program 2006: 408–14. doi:10.1182/asheducation-2006.1.408. PMID 17124091.
- Warkentin TE, Makris M, Jay RM, Kelton JG (2008). "A spontaneous prothrombotic disorder resembling heparin-induced thrombocytopenia". Am J Med 121 (7): 632–6. doi:10.1016/j.amjmed.2008.03.012. PMID 18589060.
- Keeling D, Davidson S, Watson H (May 2006). "The management of heparin-induced thrombocytopenia". Br. J. Haematol. 133 (3): 259–69. doi:10.1111/j.1365-2141.2006.06018.x. PMID 16643427.
- Warkentin TE, Heddle NM (March 2003). "Laboratory diagnosis of immune heparin-induced thrombocytopenia". Curr Hematol Rep 2 (2): 148–57. PMID 12901146.
- Warkentin TE, Aird WC, Rand JH (2003). "Platelet-endothelial interactions: sepsis, HIT, and antiphospholipid syndrome". Hematology Am Soc Hematol Educ Program: 497–519. doi:10.1182/asheducation-2003.1.497. PMID 14633796.
- Cuker A, Gimotty PA, Crowther MA, Warkentin TE (September 2012). "Predictive value of the 4Ts scoring system for heparin-induced thrombocytopenia: a systematic review and meta-analysis". Blood 120 (20): 4160–4167. doi:10.1182/blood-2012-07-443051. PMID 22990018.
- Uaprasert N, Chanswangphuwana C, Akkawat B, Rojnuckarin P (2013). "Comparison of diagnostic performance of the heparin-induced thrombocytopenia expert probability and the 4Ts score in screening for heparin-induced thrombocytopenia.". Blood Caogul Fibriolysis 24 (3): 261–8. PMID 23196326.
- Hirsh J, Heddle N, Kelton J (2004). "Treatment of heparin-induced thrombocytopenia: a critical review". Arch Intern Med 164 (4): 361–9. doi:10.1001/archinte.164.4.361. PMID 14980986.
- Weismann RE, Tobin RW (February 1958). "Arterial embolism occurring during systemic heparin therapy". AMA Arch Surg 76 (2): 219–25; discussion 225–7. doi:10.1001/archsurg.1958.01280200041005. PMID 13497418.
- Natelson EA, Lynch EC, Alfrey CP, Gross JB (December 1969). "Heparin-induced thrombocytopenia. An unexpected response to treatment of consumption coagulopathy". Ann. Intern. Med. 71 (6): 1121–5. doi:10.7326/0003-4819-71-6-1121. PMID 5391254.
- Rhodes GR, Dixon RH, Silver D (March 1973). "Heparin induced thrombocytopenia with thrombotic and hemorrhagic manifestations". Surg Gynecol Obstet 136 (3): 409–16. PMID 4688805.