Holy Trinity is an unincorporated community in Russell County, Alabama, United States. Its elevation is 312 feet (95 m), and it is located at 32°13′21″N 85°0′19″W / 32.22250°N 85.00528°W (32.2223733, -85.0052077). A post office is located in the community. It is about 20 miles south of Columbus, Georgia on State Route 165 (Alabama). It is the location of Apalachicola Fort Site, a National Historic Landmark.
The Roman Catholic institute of the Missionary Servants of the Most Holy Trinity was founded in Holy Trinity in 1928. It is also home to the Blessed Trinity Shrine, a retreat center.