Cleaves hyaluronate chains at a beta-D-GalNAc-(1->4)-beta-D-GlcA bond, ultimately breaking the polysaccharide down to 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine
This enzyme belongs to the family of lyases, specifically those carbon-oxygen lyases acting on polysaccharides. The systematic name of this enzyme class is hyaluronate lyase. Other names in common use include hyaluronidase [but cf. internal_xref(ec_num(3,2,1,35)), (hyalurononglucosaminidase) and internal_xref(ec_num(3,2,1,36)), (hyaluronoglucuronidase)], glucuronoglycosaminoglycan lyase, spreading factor, and mucinase.
Linker A, Hoffman P, Meyer K, Sampson P and Korn ED (1960). "The formation of unsaturated disacharides from mucopoly-saccharides and their cleavage to alpha-keto acid by bacterial enzymes". J. Biol. Chem.235: 3061–5. PMID13762462.
MEYER K, RAPPORT MM (1952). "Hyaluronidases". Adv. Enzymol. Relat. Subj. Biochem.13: 199–236. PMID14943668.