Hyoscyamine (6S)-dioxygenase

hyoscyamine (6S)-dioxygenase
Identifiers
EC number	1.14.11.11
CAS number	103865-33-4
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / EGO
Search PMC articles PubMed articles

In enzymology, a hyoscyamine (6S)-dioxygenase (EC 1.14.11.11) is an enzyme that catalyzes the chemical reaction

L-hyoscyamine + 2-oxoglutarate + O₂ $\rightleftharpoons$ (6S)-hydroxyhyoscyamine + succinate + CO₂

The 3 substrates of this enzyme are L-hyoscyamine, 2-oxoglutarate, and O₂, whereas its 3 products are (6S)-hydroxyhyoscyamine, succinate, and CO₂.

This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with 2-oxoglutarate as one donor, and incorporation of one atom o oxygen into each donor. The systematic name of this enzyme class is L-hyoscyamine,2-oxoglutarate:oxygen oxidoreductase ((6S)-hydroxylating). Other names in common use include hyoscyamine 6beta-hydroxylase, hyoscyamine 6beta-dioxygenase, and hyoscyamine 6-hydroxylase. This enzyme participates in alkaloid biosynthesis ii. It has 2 cofactors: iron, and Ascorbate.

References

• Hashimoto T and Yamada Y (1986). "Hyoscyamine 6beta-hydroxylase, a 2-oxoglutarate-dependent dioxygenase, in alkaloid-producing root cultures". Plant Physiol. 81 (2): 619–625. doi:10.1104/pp.81.2.619. PMC 1075387. PMID 16664866. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1075387.