INCENP

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Inner centromere protein antigens 135/155kDa
Available structures
PDB Ortholog search: PDBe, RCSB
Identifiers
Symbols INCENP ; FLJ31633; MGC111393
External IDs OMIM604411 MGI1313288 HomoloGene9624 ChEMBL: 5177 GeneCards: INCENP Gene
RNA expression pattern
PBB GE INCENP 219769 at tn.png
More reference expression data
Orthologs
Species Human Mouse
Entrez 3619 16319
Ensembl ENSG00000149503 ENSMUSG00000024660
UniProt Q9NQS7 Q9WU62
RefSeq (mRNA) NM_001040694 NM_016692
RefSeq (protein) NP_001035784 NP_057901
Location (UCSC) Chr 11:
61.89 – 61.92 Mb
Chr 19:
9.87 – 9.9 Mb
PubMed search [1] [2]
Chromosome passenger complex (CPC) protein INCENP N terminal
Identifiers
Symbol INCENP_N
Pfam PF12178
InterPro IPR022006
Inner centromere protein, ARK binding region
Identifiers
Symbol INCENP_ARK-bind
Pfam PF03941
InterPro IPR005635

Inner centromere protein is a protein that in humans is encoded by the INCENP gene.[1][2][3]

In mammalian cells, two broad groups of centromere-interacting proteins have been described: constitutively binding centromere proteins and 'passenger' (or transiently interacting) proteins.[4] The constitutive proteins include CENPA (centromere protein A), CENPB, CENPC1, and CENPD.

The term 'passenger proteins' encompasses a broad collection of proteins that localize to the centromere during specific stages of the cell cycle.[5] These include CENPE; MCAK; KID; cytoplasmic dynein (e.g., DYNC1H1); CliPs (e.g. CLIP1); and CENPF/mitosin (CENPF). The inner centromere proteins (INCENPs),[1] the initial members of the passenger protein group, display a broad localization along chromosomes in the early stages of mitosis but gradually become concentrated at centromeres as the cell cycle progresses into mid-metaphase. During telophase, the proteins are located within the midbody in the intercellular bridge, where they are discarded after cytokinesis.[3][6]

INCENP is a regulatory protein in the chromosome passenger complex. It is involved in regulation of the catalytic protein Aurora B. It performs this function in association with two other proteins - Survivin and Borealin. These proteins form a tight three-helical bundle. The N-terminal domain of INCENP is the domain involved in formation of this three-helical bundle.[7]

Interactions[edit]

INCENP has been shown to interact with H2AFZ,[8] Survivin[9] and CDCA8.[10] The ARK binding region has been found to be necessary and sufficient for binding to aurora-related kinase. This interaction has been implicated in the coordination of chromosome segregation with cell division in yeast.[11]

References[edit]

  1. ^ a b Earnshaw WC, Cooke CA (Sep 1991). "Analysis of the distribution of the INCENPs throughout mitosis reveals the existence of a pathway of structural changes in the chromosomes during metaphase and early events in cleavage furrow formation". J Cell Sci 98 (4): 443–61. PMID 1860899. 
  2. ^ Adams RR, Eckley DM, Vagnarelli P, Wheatley SP, Gerloff DL, Mackay AM, Svingen PA, Kaufmann SH, Earnshaw WC (Jul 2001). "Human INCENP colocalizes with the Aurora-B/AIRK2 kinase on chromosomes and is overexpressed in tumour cells". Chromosoma 110 (2): 65–74. doi:10.1007/s004120100130. PMID 11453556. 
  3. ^ a b "Entrez Gene: INCENP inner centromere protein antigens 135/155kDa". 
  4. ^ Choo, K. H. Andy (1997). The centromere. Oxford [Oxfordshire]: Oxford University Press. ISBN 0-19-857780-X. 
  5. ^ Earnshaw WC, Mackay AM (September 1994). "Role of nonhistone proteins in the chromosomal events of mitosis". FASEB J. 8 (12): 947–56. PMID 8088460. 
  6. ^ Cutts SM, Fowler KJ, Kile BT, Hii LL, O'Dowd RA, Hudson DF, Saffery R, Kalitsis P, Earle E, Choo KH (July 1999). "Defective chromosome segregation, microtubule bundling and nuclear bridging in inner centromere protein gene (Incenp)-disrupted mice". Hum. Mol. Genet. 8 (7): 1145–55. doi:10.1093/hmg/8.7.1145. PMID 10369859. 
  7. ^ Jeyaprakash, A. A.; Klein, U. R.; Lindner, D.; Ebert, J.; Nigg, E. A.; Conti, E. (2007). "Structure of a Survivin–Borealin–INCENP Core Complex Reveals How Chromosomal Passengers Travel Together". Cell 131 (2): 271–285. doi:10.1016/j.cell.2007.07.045. PMID 17956729.  edit
  8. ^ Rangasamy D, Berven L, Ridgway P, Tremethick DJ (April 2003). "Pericentric heterochromatin becomes enriched with H2A.Z during early mammalian development". EMBO J. 22 (7): 1599–607. doi:10.1093/emboj/cdg160. PMC 152904. PMID 12660166. 
  9. ^ Wheatley SP, Carvalho A, Vagnarelli P, Earnshaw WC (June 2001). "INCENP is required for proper targeting of Survivin to the centromeres and the anaphase spindle during mitosis". Curr. Biol. 11 (11): 886–90. doi:10.1016/S0960-9822(01)00238-X. PMID 11516652. 
  10. ^ Gassmann R, Carvalho A, Henzing AJ, Ruchaud S, Hudson DF, Honda R, Nigg EA, Gerloff DL, Earnshaw WC (July 2004). "Borealin: a novel chromosomal passenger required for stability of the bipolar mitotic spindle". J. Cell Biol. 166 (2): 179–91. doi:10.1083/jcb.200404001. PMC 2172304. PMID 15249581. 
  11. ^ Leverson JD, Huang HK, Forsburg SL, Hunter T (April 2002). "The Schizosaccharomyces pombe aurora-related kinase Ark1 interacts with the inner centromere protein Pic1 and mediates chromosome segregation and cytokinesis". Mol. Biol. Cell 13 (4): 1132–43. doi:10.1091/mbc.01-07-0330. PMC 102257. PMID 11950927. 

Further reading[edit]


This article incorporates text from the public domain Pfam and InterPro IPR005635

This article incorporates text from the public domain Pfam and InterPro IPR022006