From Wikipedia, the free encyclopedia
Jump to: navigation, search
Intersectin 1 (SH3 domain protein)
Protein ITSN1 PDB 1ki1.png
PDB rendering based on 1ki1.
Available structures
PDB Ortholog search: PDBe, RCSB
Symbols ITSN1 ; ITSN; SH3D1A; SH3P17
External IDs OMIM602442 MGI1338069 HomoloGene2277 GeneCards: ITSN1 Gene
RNA expression pattern
PBB GE ITSN1 207322 at tn.png
PBB GE ITSN1 35776 at tn.png
PBB GE ITSN1 209297 at tn.png
More reference expression data
Species Human Mouse
Entrez 6453 16443
Ensembl ENSG00000205726 ENSMUSG00000022957
UniProt Q15811 Q9Z0R4
RefSeq (mRNA) NM_001001132 NM_001110275
RefSeq (protein) NP_001001132 NP_001103745
Location (UCSC) Chr 21:
35.01 – 35.27 Mb
Chr 16:
91.73 – 91.92 Mb
PubMed search [1] [2]

Intersectin-1 is a protein that, in humans, is encoded by the ITSN1 gene.[1][2]

The protein encoded by this gene is a cytoplasmic membrane-associated protein that indirectly coordinates endocytic membrane traffic with the actin assembly machinery. In addition, the encoded protein may regulate the formation of clathrin-coated vesicles and could be involved in synaptic vesicle recycling. This protein has been shown to interact with dynamin, CDC42, SNAP23, SNAP25, SPIN90, EPS15, EPN1, EPN2, and STN2. Multiple transcript variants encoding different isoforms have been found for this gene, but the full-length nature of only two of them have been characterized so far.[3]


ITSN1 has been shown to interact with SNAP-25,[4] SOS1,[5] SCAMP1[6] and CDC42.[7][8]


  1. ^ Guipponi M, Scott HS, Chen H, Schebesta A, Rossier C, Antonarakis SE (December 1998). "Two isoforms of a human intersectin (ITSN) protein are produced by brain-specific alternative splicing in a stop codon". Genomics 53 (3): 369–76. doi:10.1006/geno.1998.5521. PMID 9799604. 
  2. ^ Yamabhai M, Hoffman NG, Hardison NL, McPherson PS, Castagnoli L, Cesareni G, Kay BK (December 1998). "Intersectin, a novel adaptor protein with two Eps15 homology and five Src homology 3 domains". J Biol Chem 273 (47): 31401–7. doi:10.1074/jbc.273.47.31401. PMID 9813051. 
  3. ^ "Entrez Gene: ITSN1 intersectin 1 (SH3 domain protein)". 
  4. ^ Okamoto, M; Schoch S; Südhof T C (June 1999). "EHSH1/intersectin, a protein that contains EH and SH3 domains and binds to dynamin and SNAP-25. A protein connection between exocytosis and endocytosis?". J. Biol. Chem. (UNITED STATES) 274 (26): 18446–54. doi:10.1074/jbc.274.26.18446. ISSN 0021-9258. PMID 10373452. 
  5. ^ Tong, X K; Hussain N K, de Heuvel E, Kurakin A, Abi-Jaoude E, Quinn C C, Olson M F, Marais R, Baranes D, Kay B K, McPherson P S (March 2000). "The endocytic protein intersectin is a major binding partner for the Ras exchange factor mSos1 in rat brain". EMBO J. (ENGLAND) 19 (6): 1263–71. doi:10.1093/emboj/19.6.1263. ISSN 0261-4189. PMC 305667. PMID 10716926. 
  6. ^ Fernández-Chacón, R; Achiriloaie M; Janz R; Albanesi J P; Südhof T C (April 2000). "SCAMP1 function in endocytosis". J. Biol. Chem. (UNITED STATES) 275 (17): 12752–6. doi:10.1074/jbc.275.17.12752. ISSN 0021-9258. PMID 10777571. 
  7. ^ Hussain, N K; Jenna S, Glogauer M, Quinn C C, Wasiak S, Guipponi M, Antonarakis S E, Kay B K, Stossel T P, Lamarche-Vane N, McPherson P S (October 2001). "Endocytic protein intersectin-l regulates actin assembly via Cdc42 and N-WASP". Nat. Cell Biol. (England) 3 (10): 927–32. doi:10.1038/ncb1001-927. ISSN 1465-7392. PMID 11584276. 
  8. ^ Snyder, Jason T; Worthylake David K; Rossman Kent L; Betts Laurie; Pruitt Wendy M; Siderovski David P; Der Channing J; Sondek John (June 2002). "Structural basis for the selective activation of Rho GTPases by Dbl exchange factors". Nat. Struct. Biol. (United States) 9 (6): 468–75. doi:10.1038/nsb796. ISSN 1072-8368. PMID 12006984. 

Further reading[edit]