Image restoration is different from image enhancement in that the latter is designed to emphasize features of the image that make the image more pleasing to the observer, but not necessarily to produce realistic data from a scientific point of view. Image enhancement techniques (like contrast stretching or de-blurring by a nearest neighbor procedure) provided by "Imaging packages" use no a priori model of the process that created the image.
With image enhancement noise can effectively be removed by sacrificing some resolution, but this is not acceptable in many applications. In a Fluorescence Microscope resolution in the z-direction is bad as it is. More advanced image processing techniques must be applied to recover the object.
Increasing resolution, especially in the axial direction Removing noise Increasing contrast
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