Business Loop 44 through Sarcoxie begins running south from I-44 concurrent with Route 37 It then splits off at Blackberry St., which becomes High Street upon entering Sarcoxie. After leaving the town, BUS I-44 angles northeast and ends at I-44. This road is part of former U.S. Route 166 which preceded I-44 through the area.
Business Loop 44 in Springfield begins at the eastern terminus of Route 266 and runs east along Chestnut Expressway, intersecting U.S. Route 160 and Route 13. It then runs along the north edge of downtown Springfield. I-44 BUS turns north at Glenstone Avenue, bumping Business Route 65. It then ends where Glenstone meets I-44.
Business Loop 44 in Lebanon begins at the westernmost Lebanon exit and ends at the eastern most exit. The route follows former U.S. Route 66 its entire length. Business I-44 follows Springfield Road, then curves off and becomes Elm Street. At Jefferson Street is a junction with Routes 5, 32, and 64.
Business Loop 44 in the Waynesville-St. Robert area begins on the west side of Waynesville at Exit 156. The road briefly heads north on Ichord Avenue, then heads east in a duplex with Route 17. The road, at this point, also becomes Historic Route 66. Business I-44 continues east, past the Pulaski County Courthouse in the old part of the city (where Route 17 turns off north). The route then climbs a steep hill, and rounds a curve underneath a rock overhang painted like a frog. Business I-44 continues into St. Robert, the lanes split with a Roadside Park between them. It pass on through St. Robert, ending at Exit 161, which is also the exit for Business Spur I-44.
Business Spur 44 is a short route connecting Interstate 44 with the main north gate of Ft. Leonard Wood. The St. Robert stretch is lined with businesses and is called Missouri Avenue whose name continues into Ft. Leonard Wood. It is an old alignment of Route 17.
Business Loop 44 in Rolla begins at the westernmost Rolla exit and ends at the easternmost exit. The route follows US Route 66 its entire length. The road is Kingshighway to Bishop Avenue, where the road joins northbound US 63, with which it is duplexed to I-44. Near the Missouri University of Science and Technology is a model of Stonehenge.
Business Loop 44 is a former routing of U.S. Route 66 through downtown Pacific, Missouri. This portion of road is designated a Business Loop after Interstate 44 was built to bypass the town. U.S. Route 50 was then rerouted onto Interstate 44, making this portion a Business Loop after US 66 was removed from the area in 1979. After the Business Loop's western terminus, the road continues solely as Historic U.S. Route 66 until an intersection with Route 100.