The main painted altarpieces attributed to Jorge Afonso were commissioned by the old Queen Leonor, widow of King John II and sister of Manuel I. For the former Queen, Jorge Afonso painted the main altarpiece of the Convent of Madre de Deus, in Lisbon, in 1515. This magnificent altarpiece is now in the National Museum of Ancient Art, in Lisbon. Between 1520 and 1530, Jorge Afonso painted the 14 panels of the main altarpiece of the Monastery of Jesus, in Setúbal, again sponsored by Queen Leonor. The panels can be seen in the museum of the Monastery.
Jorge Afonso's workshop is also linked to the painted decoration of the walls of the Round Church of the Convent of Christ, in Tomar, executed in the 1530s.