Kallikrein

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Kallikreins are a subgroup of serine proteases, enzymes capable of cleaving peptide bonds in proteins. In humans, plasma kallikrein (KLKB1) has no known homologue, while tissue kallikrein-related peptidases (KLKs) encode a family of fifteen closely related serine proteases. These genes are localised to chromosome 19q13, forming the largest contiguous cluster of proteases within the human genome. Kallikreins are responsible for the coordination of various physiological functions including blood pressure, semen liquefaction and skin desquamation.

Occurrence[edit]

Eugen Werle reported in 1934 finding a substance in the pancreas of humans and various animals in such great amounts that the pancreas could be taken for its site of origin. He named it kallikrein, by derivation from the Greek word for pancreas. Since then similar enzymes have been found in the biological fluids of humans and other mammals, as well as in some snake venoms.[1]

Venom[edit]

The caterpillar known as Lagoa crispata contains poison glands attached to hypodermic spines, which produce and inject venom that has been characterized as kallikrein in nature.[2]

Plasma kallikrein[edit]

The KLKB1 gene encoding plasma kallikrein is located on chromosome 4q34-35. It is synthesised as an inactive precursor, prekallikrein, which must undergo proteolytic processing to become activated. This is facilitated by factor XII, PRCP or other stimuli.

Plasma kallikrein liberates kinins (bradykinin and kallidin) from the kininogens,[3][4] peptides responsible for the regulation of blood pressure and activation of inflammation. It is also capable of generating plasmin from plasminogen:

Fibrinolysis (simplified). Blue arrows denote stimulation, and red arrows inhibition.

Tissue kallikreins[edit]

Distinct from plasma kallikrein, tissue kallikreins (KLKs) are expressed throughout the human body and perform various physiological roles. As some kallikreins are able to catalyse the activation of other kallikreins, several cascades involving these proteases have been implicated in the regulation of homeostatic functions.

Function[edit]

Similar to KLKB1, three tissue kallikreins KLK1, KLK2 and KLK12 also participate in regulation of blood pressure via the activation of bradykinin.[5] KLK2, KLK3, KLK4, KLK5 and KLK14 are expressed in the prostate and are thought to be responsible for regulating semen liquefaction through hydrolysis of seminogelin.[6][7] Desquamation of the skin is likely controlled by KLK5, KLK7 and KLK14, which are expressed in the outermost layer of the epidermis and cleave cellular adhesion proteins.[8] Additionally, KLK6 and KLK8 are associated with neuronal plasticity in the central nervous system.[9]

Genes[edit]

There are 15 known human tissue kallikreins: KLK1, KLK2, KLK3, KLK4, KLK5, KLK6, KLK7, KLK8, KLK9, KLK10, KLK11, KLK12, KLK13, KLK14, KLK15

Clinical significance[edit]

Kallikrein-related peptidases are targets of active investigation by drug researchers as possible biomarkers for cancer.[10][11]

Prostate-specific antigen (PSA; hk3, human kallikrein gene 3) and human glandular kallikrein (hK2) are used as tumor markers for prostate cancer.

See also[edit]

References[edit]

  1. ^ Raspi, Giorgio "Kallikrein and kallikrein-like proteinases: purification and determination by chromatographic and electrophoretic methods" Journal of Chromatography B 684: 265-287, 1996. http://www.icb.ufmg.br/prodabi/prodabi5/homepages/liza/artigos/review2.pdf, retrieved 31 Aug 2010
  2. ^ Lamdin, J.M. et al., “The venomous hair structure, venom and life cycle of Logoa crispata, a puss caterpillar of Oklahoma” Toxicon 38:1163-1189, 2000.
  3. ^ Bhoola, K.D., Figueroa, C.D., Worthy, K. "Bioregulation of kinins: kallikreins, kininogens, and kininases" Pharmacological Reviews 44: 1-80, 1992.
  4. ^ Stefan Offermanns; Walter Rosenthal (2008). Encyclopedia of Molecular Pharmacology. Springer. pp. 673–. ISBN 978-3-540-38916-3. Retrieved 11 December 2010. 
  5. ^ Giusti B, Serratì S, Margheri F, Papucci L, Rossi L, Poggi F, Magi A, Del Rosso A, Cinelli M, Guiducci S, Kahaleh B, Matucci-Cerinic M, Abbate R, Fibbi G, Del Rosso M. (Nov 2005). "The antiangiogenic tissue kallikrein pattern of endothelial cells in systemic sclerosis". Arthritis Rheum 52 (11): 3618–28. doi:10.1002/art.21383. PMID 16255054. 
  6. ^ Michael IP, Pampalakis G, Mikolajczyk SD, Malm J, Sotiropoulou G, Diamandis EP. (May 2006). "Human tissue kallikrein 5 is a member of a proteolytic cascade pathway involved in seminal clot liquefaction and potentially in prostate cancer progression.". J Biol Chem 281 (18): 12743–50. doi:10.1074/jbc.M600326200. PMID 16517595. 
  7. ^ Emami N, Diamandis EP (Feb 2008). "Human kallikrein-related peptidase 14 (KLK14) is a new activator component of the KLK proteolytic cascade. Possible function in seminal plasma and skin". J Biol Chem. 8;283 (6): 3031–41. doi:10.1074/jbc.M707253200. PMID 18056261. 
  8. ^ Ovaere P, Lippens S, Vandenabeele P, Declercq W (Aug 2009). "The emerging roles of serine protease cascades in the epidermis". Trends Biochem Sci 34 (9): 453–63. doi:10.1016/j.tibs.2009.08.001. PMID 19726197. 
  9. ^ Tamura H, Ishikawa Y, Hino N, Maeda M, Yoshida S, Kaku S, Shiosaka S (Feb 2006). "Neuropsin is essential for early processes of memory acquisition and Schaffer collateral long-term potentiation in adult mouse hippocampus in vivo". J Physiol. 1;570 (3): 541–51. doi:10.1113/jphysiol.2005.098715. PMC 1479887. PMID 16308352. 
  10. ^ Borgono, C.A., and Diamandis, E.P. "The emerging roles of human tissue kallikreins in cancer" Nature Reviews Cancer 4: 876-90, 2004.
  11. ^ Diamandis E.P., Yousef G.M. (2002). "Human Tissue Kallikreins: A Family of New Cancer Biomarkers". Clinical Chemistry 48: 1198–1205. 

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