Kan Lal Agarwal

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Dr. Kanhiya (Kan) Lal Agarwal (February 4, 1941 – June 22, 2003) was an Indian-born American biochemist, he was professor at the Department of Biochemistry and Molecular Biology and Chemistry at the University of Chicago and did consulting work for the National Institutes of Health. At MIT he worked on the development of the artificial gene as part of team of 1968 Nobel laureate H. Gobind Khorana.

Personal life[edit]

Kanhiya Lal Agarwal was born in an Lal Choudhary Jaipur, India in 1941. Born Kanhiya Lal Choudhary, he changed his name in elementary school to Agarwal (since Choudhary is within same cast of Agarwals). His grandfather was a politician and his father was a civil engineer for the public. He became a US citizen in 1975. His only family in the U.S. was his brother Brij Mohan Choudhary (1943–1991), his nephew Brian and his sister-in-law Mira Zivkovich (Choudhary). The rest of family still reside in India.

Agarwal died in Chicago, Sunday June 22, 2003 from a heart attack.[1][2]

Career[edit]

2006-11 Ken Agarwal Award Student Winner with classmates
More 2006-2011 Ken Agarwal Award Korean Student Winner with faculty

Kanhiya Lal Agarwal - post doctoral student at MIT from 1969 to 1974 where he in the team of with 1968 Nobel laureate H. Gobind Khorana on the development of the artificial gene.[3][4] Agarwal joined the staff at the University of Chicago where in 1974 as an Assistant Professor Departments of Biochemistry, Biophysics and Theoretical Biology, and the Committee on Genetics. At the time of his death he was Professor Emeritus in the Department of Biochemistry and Molecular Biology and Chemistry. Curriculum Committee. He served on the Departmental Seminar Committee, Graduate Students' Preliminary Examination Committee, Curriculum Committee, Appointments and Promotions Committee where he eventually became chairmen. He was in charge of departmental seminars and advised graduate Students.[3] He was awarded the Gold Key award from the university Alumni association.[3] Agarwal worked as a consultant for Abbott Laboratories and Luck Biotech as well as serving on three study sections for the National Institutes of Health.[3] Kan L Agarwal Award/Scholarship at the Korea Advanced Institute of Science and Technology (KAIST)

Kanhiya Lal Agarwal favorite students at the University of Chicago who worked as his understudy were South Korean, who later established Korea Advanced Institute of Science and Technology (KAIST) was established in 1971 as the nation’s first graduate school specializing in science and engineering education and research.

As the gratitude to Kan's contribution, a check for $5,000USD they started an award and scholarship for the best Korean student from the KAIST institute to go to University of Chicago entitled the Kan L Agarwal Award.

Honors[edit]

  • 1975-1976 Mellon Foundation Faculty Fellows
  • 1976-1981 Research Career Development Award (NIH)
  • 1995 The University of Chicago Alumni Association, Gold Key Award for outstanding faculty

Publications[edit]

Publications US[edit]

  • Agarwal, K. and Dhar, M.M. (1963) Nature of products formed by the action of polyphosphate ester on nucleotides. Indian J. Chem. 1, 451.
  • Agarwal, K. and Dhar, M.M. (1964) Polymerization of adenosine 3'-and 5' phosphates through p-nitrophenyl and picryl esters. Indian J. Chem. 2, 493.
  • Agarwal, K. and Dhar, M.M. (1965) The use of 2',3'-anhydrounucleosides in the synthesis of the internucleotide bond. Tetrahedron Letts. 2451.
  • Nagpal, K.L. and Agarwal, K., and Dhar, M.M. (1965) Synthesis of orotic esters and amides. Indian J. Chem. 3, 356.
  • Agarwal, K. and Dhar, M.M. (1965) The selective esterification of the 5'-hydroxyl of thymidine. Experientia 432.
  • Dhar, M.M. and Agarwal, K. (1964) The synthesis of ?-poly-y-cholesterol-L glutamate and ?-poly-?-sitoseryl-L-glutamate. Steroids 139.
  • Agarwal, K. and Dhar, M.M. (1965) The synthesis of ?-poly-lysine steroid esters. Steroids 4,495.
  • Agarwal, K. and Dhar, M.M. (1965) The synthesis of ?-poly-cholic acidamido-L-lysine and a-poly-deoxycholic acid-amindo-L-lysine. Steroids 6,105.
  • Agarwal, K. (1966) Synthesis of nucleotide nitrogen mustards. Indian J. Chem. 4, 325.
  • Agarwal, K., Kenner, G.W. and Sheppard, R.C. (1968) Synthesis of five heptadecapeptides related to human gastrin. J. Chem. Soc. C, 1384.
  • Agarwal, K., Beecham, J., Bentley, P.H., Gregory, R.A., Kenner, G.W., Sheppard, R.C. and Tracy, *H.J. (1968) Isolation structure and synthesis of ovine and bovine gastrins. Nature 219, 614.
  • Agarwal, K., Kenner, G.W. and Sheppard, R.C. (1969) Structure and synthesis of canine gastrin. Experientia 346.
  • Agarwal, K., Johnstone, R.A.W., Kenner, G.W., Millington, D.S. and Sheppard, R.C. (1968) Mass spectromy of N-methylated peptide derivatives. Nature 219, 498.
  • Agarwal, K., Kenner, G.W. and Sheppard, R.C. (1969) Feline gastrin. An example of peptide sequence analysis by mass spectrometry. J. Amer. Chem. Soc. 91, 3096.
  • Gregory, R.A., Tracy, H.J., Agarwal, K. and Grossman, M.I. (1969) Amino Acid constitution of two gastrins isolated from Zollinger-Ellison tumor tissue. Gut. 10, 603.
  • Agarwal, K., Kenner, G.W. and Sheppard, R.C. (1969) Synthesis of canine gastrin and a new synthesis of human gastrin. J. Chem. Soc. (C), 2213.
  • Agarwal, K., Kenner, G.W. and Sheppard, R.C. (1969) Synthesis of the decapeptide, Leu-Ala-Ala-GIy-Lys-Val-Glu-Asp-Ser-Asp. J. Chem. Soc. (C), 2218.
  • Agarwal, K., Grudzinski, G., Kenner, G.W., Rogers, N.H., Sheppard, R.C. and McGuigan, J.E. (1971) Immunological differentiation between gastrin and related peptide hormones through a novel conjugation of peptides to proteins. Experientia 514.
  • Agarwal, K., Buchi, H., Caruthers, M.H., Gupta, N., Khorana, H.G., Kleppe, K., Kumar, A., Ohtsuka, E., Rajbhandary, U.L., van de Sande, J.H., Sgaremella, V., Weber, H. and Yamada, T. (1970) Total synthesis of the gene for an alanine transfer ribonucleic acid from yeast. Nature 227, 27.
  • Agarwal, K., Yamazaki, A. and Khorana, H.G. (1971) A convenient and general method for the preparation of protected dideoxyribonucleotides containing 5'-phosphate end groups J. Amer. Chem. Soc. 93, 2754.
  • Besmer, P., Agarwal, K., Caruthers, M.H., Cashion, P.J., Fridkin, M., Jay, E., Kumar, A., Loewen, P.C., Ohtsuka, E., van de Sande, J.H., Sidorova, N., RajBhandary, U.L., Khorana, H.G. (1971) *Progress in the total synthesis of the gene suppressor transfer RNA. Fed. Proc. 30, 413.
  • Cashion, P.J., Fridkin, M., Agarwal,K., Jay, E., Jay, L. and Khorana, H.G. (1972). Resolution of oligonucleotide mixtures on 0-trityl and napthyl-substituted cellulose. CARB 27, 163rd ACS National Meeting, Boston.
  • Jay, E., Agarwal, K., Cashion, P.J., Fridkin, M., Jay, L. and Khorana, H.G. (1972) Newer methods in oligonucleotide synthesis. GARB 28, ACS National Meeting, Boston.
  • Agarwal, K., Kumar, A. and Khorana, H.G. (1972) Total synthesis of the gene for an alanine transfer ribonucleic acids from yeast (5). Synthesis of a deoxydodecanucleotide and a sequence 46-65. J. Mol. Biol. 72, 351.
  • Caruthers, M.H., Kleppe, van de Sande, J.H., Sgaramella, V., Agarwal, K., Bucchi, H., Gupta, N.K., Kumar, A., Ohtsuka, E., RajBhandary, U.L., Terao, T., Weber, H., Yamada, T. and Khorana, H.G. (1972) Total synthesis of the structural gene for an alanine transfer RNA from yeast (13). Enzymatic joining to form the total DNA duplex. J. Mol. Biol. 72, 209.
  • Khorana, H.G., Agarwal K., Buchi, H., Caruthers, M.H., Gupta, N., Kleppe, K., Kumar, A., Ohtsuka, E., RajBhandary, U.L., van de Sande, J.H., Sgaramella, V., Terao, T., Weber, H. and Yamada, T. (1972) The total synthesis of the structural gene for an alanine transfer ribonucleic acid from yeast. J. Mol. Biol. 72, 209.
  • Agarwal, K. and Khorana, H.G. (1972) The use of aromatic isocyanates for selective blocking of the terminal 3'-hydroxyl group in protected deoxyribooligonucleotides. J. Amer. Chem. 94, 3578.
  • Cashion P.J., Fridkin, M., Agarwal, K., Jay, E. and Khorana, H.G. (1972) Resolution of oligonucleotide mixtures on o-trityl- and napthyl-substituted cellulose. Biochemistry 12, 1985.
  • Agarwal, K., Yamazaki, A., Cashion, P.J. and Khorana, H.G. (1972) Chemical synthesis of polynucleotides. Agnew. Chem. 11, 451.
  • Agarwal, K., Fridkin, M., Jay, E. and Khorana, H.G. (1973) Deoxyoligonucleotide synthesis using a new phosphate protecting group. J. Amer. Chem. Soc. 95, 2020.
  • Caruthers, M.H., Agarwal, K., Besmer, P., Cashion, P.J., Fridkin, M., Jay, E., Kumar, A., Loewen, P., Miller,R., Minamoto, K., Ramamoorthy, B., Panet, A., van de Sande, J.H., Sekiya, T.,Siderova, *N. and Khorana, H.G. (1973) Total synthesis of the gene for the precursor of E. coli tyrosine suppressor transfer RNA. 9th Intl. Congress of Biochemistry, Stockholm, Abstract 3010, pg. 194.
  • Agarwal, K., Besmer, P., Buchi, H., Caruthers, M.H., Cashion, P.J., Fridkin, M., Jay, E., Kleid, D.G., Sekiya, T., Siderova, N. and Khorana, H.G. (1973) Synthesis of the gene for the precursor of E. coli tyrosine suppressor transfer RNA. CARB, 29, ACS National Meeting, Chicago.
  • Fridkin, M., Cashion, P.J., Agarwal, K., Jay, E. and Khorana, H.G. (1974) Rapid separation of oligonucleotide mixtures on aromatic cellulose derivatives. Methods in Enzymology B. Williams and M. Wilcheck (eds.).
  • Agarwal, K., Berlin, Y.A., Kleid, D.G., Smirnov, V.D. and Khorana, H.G. (1975) The synthesis of a DNA duplex corresponding to the icosanucleotide sequence at the end 5-end of the messenger RNA from the gene N of bacteriophage. J. Biol. Chem. 250, 5563.
  • Kleid, D.G., Agarwal, K. and Khorana, H.G. (1975) The nucleotide sequence in the operator region of the gene N of bacteriophage. J. Biol. Chem. 250, 5574.
  • Khorana, H.G., Agarwal, K., Besmer, P., Caruthers, M.H., Cashion, P.J., Fridkin, M., Jay, E., Kumar, A., Loewen, P., Miller, R., Minamoto, R., Ramamoorthy, B., Panet, A., vande Sande, J.H., *Sekiya, T., Siderova, N. (1976) Total synthesis of the structural gene for the precursor of a tyrosine suppressor transfer RNA from E. coli. J. Biol. Chem. 251, 565.
  • Jay, E., Cashion, P.J., Fridkin, M., Ramamoorthy, B., Agarwal, K., Caruthers, M.H. and Khorana, H.G. (1976) Total synthesis of DNA corresponding to the precursor for an E. coli tyrosine suppressor tRNA (5). Synthesis of the deoxynucleotide segments representing the nucleotide sequence 71-103. J. Biol. Chem. 251, 609.
  • Agarwal, K., Caruthers, M.H., Buchi, H., van de Sande, J.H. and Khorana, H.G. (1976) Total synthesis to the DNA corresponding to the precursor to the E. coli tyrosine suppressor tRNA (6). Synthesis of the segments corresponding to the nucleotide sequence 100-126. J. Biol. Chem. 251, 624.
  • Agarwal, K., Caruthers, M.H., Fridkin, M., Kumar, A., van de Sande, J.H. and Khorana, H.G. (1976). Total synthesis of the structural gene for the precursor of a tyrosine suppressor transfer RNA from E. coli. Synthesis of polynucleotide segments corresponding to the nucleotide sequence 47-78. J. Biol. Chem. 251, 599.
  • Agarwal, K., Belagaje, R., Berlin, Y.A., Gait, M.J., Kleid, D.G., Lees, R.G., Norris, K.E., Smirnov, V. and Khorana, H.G. (1976) A rapid and convenient method for the synthesis of deoxyribooligonucleotides carrying a 5'-phosphate end group using a new protecting group. J. Biol. Chem. 98, 1065.
  • Snopek, T.J., Sugino, A., Agarwal, K. and Cozzarelli, N.R. (1976) Catalysis of DNA joining by bacteriophage T4 RNA ligase. Biochem. Biophys. Res. Commun. 68, 417.
  • Agarwal, K. and Riftina, R. (1978) Chemical synthesis of a self-complementary octanucleotide, dG-G-T-T-A-A-C-C, by a modified triester method. Nucl. Acids Res. 5, 2809.
  • Noyes, B.E., Mevarech, M., Stein, R. and Agarwal, K. (1979) Detection and partial sequence analysis of gastrin mRNA using an oligonucleotide probe. Proc. Natl. Acad. Sci. USA 76, 1770.
  • Hines, J.L., Chauncey, T.R. and Agarwal, K. (1979) Purification and properties of the Hpa I and Hpa II endonucleases. Methods in Enzymology, L. Grossman and K. Moldave, eds. Vol. 65 Part 1, p. 153.
  • Agarwal, K. and Riftina, R. (1979) Synthesis and enzymatic properties of deoxyribooligonucleotides containing methyl and phenylphosphonate linkages. Nucl. Acids Res. 6, 3009.
  • Mevarech, M., Noyes, B.E. and Agarwal, K. (1979) Detection of gastrin-specific mRNA using oligonucleotide probes of defined sequence. J. Biol. Chem. 254, 7472.
  • Chan, S.J., Noyes, B.E., Agarwal, K. and Steiner, D.F. (1979) Construction and selection of recombinant plasmids containing full length cDNA corresponding to rat insulins I and II. Proc. Natl. Acad. Sci. USA. 76, 5036.
  • Yoo, O.J. and Agarwal, K. (1980) Cleavage of single-strand oligonucleotides and bacteriophage ?X174 DNA by Msp I endonuclease. J. Biol. Chem. 255, 10559.
  • Agarwal, K., Brunstedt, J. and Noyes, B.E. (1981) A general method for detection and characterization of an mRNA using an oligonucleotide probe. J. Biol. Chem. 256, 1023.
  • Agarwal, K. and Noyes, B.E. (1980) Studies on gastrin mRNA structure using an oligonucleotide probe. J. Annals of N.Y. Acad. Sci. 343, 433.
  • Chan, S.J., Noyes, B.E., Agarwal, K., Ackerman, E., Quinn, P., Keim, P., Sigler, P.B., *Heinrikson, R.L. and Steiner, D.F. (1980) Elucidation of primary structures of the initial translation products of the rat insulin genes. Int. Congr. Ser. Experpta. Med., 500, 113.
  • Steiner, D.F., Patzelt, C., Chan, S.J., Quinn, P., Tager, H.S., Nielsen D., Lernmark, A., Noyes, B.E., Agarwal, K., et al. (1980) Formation of biologically active peptides. Proc. Royal Soc. London Ser. B., 210, 45.
  • Agarwal, K. and Noyes, B.E. (1981) Studies on gastrin mRNA - an illustrative approach. In: Gut *Hormones. S.R. Bloom and J.M. Polak (eds.), 2nd edition, Churchill Livingstone, Edinburgh, p. 49.
  • Schaffer, M.H., Agarwal, K. and Noyes, B.E. (1982) The amino acid sequence of rat gastrin determined from the nucleotide sequence of the mRNA. Peptides 3, 693.
  • Yoo, O.J., Powell, C.T. and Agarwal, K. (1982) Molecular cloning and nucleotide sequence of full length cDNA coding for porcine gastrin hormone. Proc. Nat!. Acad. Sci. USA. 79, 1049.
  • Yoo, O.J., Dwyer-Hallquist, P. and Agarwal, K. (1982) Purification and properties of the Hpa I methylase. Nucl. Acids Res. 10, 6511.
  • Dwyer-Hallquist, P., Kezdy, F.J. and Agarwal, K. 91982) Interaction of the Hpa I endonuclease with synthetic oligonucleotides. Biochemistry 21, 4693.
  • Agarwal, K., Hrinyo, T.P. and Yang, N.C. (1983) Interaction of (±)-7y,8t-dihydroxy-9t, 10t-epoxy-7,8,9,10-tetrahydrobenzo(a)pyrene with relaxed circular pBR322 DNA. Biochem. Biophys. Res. Commun. 144, 14.
  • Ito, R., Sato, K., Helmer,T., Jay, G. and Agarwal, K. (1984) Structural analysis of the gene encoding human gastrin: The large intron contains an Alu sequence. Proc. Natl. Acad. Sci. USA. 81, 4662.
  • Katti, S.B. and Agarwal, K. (1985) N,N-Bis (2-oxo-3-oxazoIidinyl)phosphoramidic chloride (BOPDC): A novel coupling reagent in the synthesis of oligodeoxyribonucleotides. Tett. Lett., 26, 2547.
  • Powell, C.T., Ney, C., Aran, P. and Agarwal, K. (1985) A gastrin gene is expressed in both porcine pituitary and antral mucosal tissues. Nucl. Acids Res., 13, 7299.
  • Callahan, L., Han, F.-S., Watt, W., Duchamp, D., Kezdy, F.J. and Agarwal, K. (1986) B-Z DNA transition probed by oligonucleotides containing methyl phosphonates. Proc. Natl. Acad. Sci. USA., 83, 1617.
  • Sato, K., Ito, R., Baek,K.-H. and Agarwal, K. (1986) A specific DNA sequence controls termination of transcription in the gastrin gene. Mol. Cell. Biol., 6, 1032.
  • Katti, S.B. and Agarwal, K. (1986) Separation of disatereomers of methylphosphonate dinucleotides. Tett. Lett., 27, 5327-5330.
  • Baek, K.-H., Sato, K., Ito, R. and Agarwal, K. (1986) RNA polymerase II transcription terminates at a specific DNA sequence in a Hela cell-free reaction. Proc. Natl. Acad. Sci. USA 83, 7623-7627.
  • Sato, K., Ito, R., Agarwal, K., and Hosokawa, K. (1988) Mechanism of transcription termination in eukaryotic cell. Nucl. Acids Res. 19, 185-201.
  • Callahan, L., Geller, D.H., Ng, K., Agarwal, K. and Schwartz, N.B. (1988) Synthesis and properties of a nonhydrolysable adenosinephosphosulfate analog. Anal. Biochem. 177, 67-71.
  • Kang, S-C., Agarwal, K., and Yoo, O.J. (1989) Molecular cloning and sequence analysis of cDNA coding for canine gastrin. Biochem. Int. 18, 631-636.
  • Han, F-S., Walt, W, Duchamp, D.J., Callahan, L., Kedzy, F.J. and Agarwal, K. (1990) Molecular structure of [dCp(CH3)G]. A neutral dinucleotide with Watson-Crick base pairing and a right handed helical twist. Nucl. Acids Res., 18, 2759-2767.
  • Yoo, 0., Yoon, H., Baek, K., Jeon, C.J., Miyamoto, K., Ueno, A. and Agarwal, K. (1991) Cloning, expression and characterization of the human transcription elongation factor, TFIIS. Nucl. Acids Res. 19, 1073-1079.
  • Agarwal, K., Baek, K.H., Jeon, C.J., Miyamoto, K., Ueno, A. and Yoon, H.S. (1991) Stimulation of transcript elongation requires both the zinc finger and RNA polymerase 11 binding domains of human TFIIS. Biochemistry, 30, 7842-7851.
  • Ueno, A., Baek, K.H., Jeon, C.J. and Agarwal, K. (1992) Netropsin specifically enhances RNA polymerase II termination at terminator sites in vitro. Proc. Natl. Acad. Sci. USA. 89, 3676-3680.
  • Montag, A.G., Oka, T., Baek, K.H., Choi, C.S., Jay, G. and Agarwal, K. (1993) Tumors in hepato-biliary tract and pancreatic islet tissues of transgenic mice harboring gastrin SV40 T antigen fusion gene. Proc. Natl. Acad. Sci. 90,6696-7000.
  • Qian,X., Jeon, C.J., Yoon, H.S., Agarwal, K. and Weiss, M.A. (1993) The Zn ribbon: structure of a novel nucleic acid binding motif in eukaryotic transcriptional elongation factor TFIIS. Nature 365, 277-279.
  • Qian,X., Gozani, S., Yoon, H.S., Jeon, C.J., Agarwal, K. and Weiss, M.A. (1993) A novel zinc finger motif in the basal transcriptional machinery: three dimensional NMR studies of the nucleic acid binding domain of human transcriptional elongation factor TFIIS. Biochemistry 32, 9944-9959.
  • Park, H. R., Baek, K.H., Jeon, C.J., Agarwal, K. and Yoo, O.J. (1994) Characterization of the gene encoding human transcriptional elongation factor TFIIS gene. Gene 2, 260-267.
  • Jeon, C.J., Yoon, H.S. and Agarwal, K. (1994) The TFIIS zinc ribbon dipeptide asp-glu is critical for stimulation of elongation and RNA cleavage by RNA polymerase II. Proc. Natl. Acad. Sci USA 91, 9106-9110.
  • Jeon, C.J. and Agarwal, K. (1996) Fidelity of RNA polymerase II transcription controlled by elongation factor TFIIS. Proc. Natl. Acad. Sci. USA 93,13677-13682.
  • Yoon, H.S., Sitikov, A.S., Jeon, C.J. and Agarwal, K. (1998) Preferential interaction of rnRNA proofreading factor TFIIS zinc ribbon with rU•dA base-pairs correlates with its function. Biochemistry. 37, 12104-12112.
  • Jeon C.J., Sitikov, A., Yoon, H.S. and Agarwal, K. (1998) The mRNA proofreading factor TFIIS zinc ribbon binds to nucleic acid ends without requiring zinc. Biochemistry In progress.

References[edit]

External links[edit]