|The factual accuracy of parts of this article (those related to article) may be compromised due to out-of-date information. (August 2011)|
Construction of the line for the former Hellenic State Railways started in January 1951 and was completed in 1954. It is a branch of the Thessaloniki–Bitola railway, which began operations in 1894. The line serves the town of Ptolemaida, and connects to the lignite-fired power plants of Public Power Corporation (ΔΕΗ) at Komanos freight station. The terminal station is in the southern part of the city of Kozani.
Industrial branch lines connect to the PPC power plants of Ptolemais and Agios Dimitrios, normally used by freight trains carrying light fuel oil. Another branch line to the former fertilizer plant of AEVAL is disused. The line is also used by freight trains carrying sugar beats to Platy.
The Kozani–Amyntaio line was under renovation between 2003 - 2007. On January 22, 2007, it was reopened with three trains daily, which departed from Kozani, arriving in Thessaloniki in three hours (instead of four hour before the renovation). A comparable bus trip between Kozani and Thessaloniki via Egnatia Motorway takes 1.5 hours. Since 1 August 2009 the passenger service was reduced to only one train per day  and the passenger services were terminated in 2010. Infrequent freight trains still use the line to reach the PPC power generating stations.
Construction of a line from Kozani to Kalampaka is still under consideration.
- "Rail link in western Macedonia reopened". Athens News Agency. 2007-01-19. Retrieved 2008-06-08.
- TrainOSE S.A. "Timetables 1 August 2009", Tables 8A/8B
- "Σε λειτουργία και πάλι η σιδηροδρομική γραμμή Αμύνταιο - Κοζάνη" (in Greek). Travel Daily News. 2007-01-22. Retrieved 2008-04-12.
- I. Zartaloudis, D. Karatolos, D. Koutelidis, G. Nathenas, S. Fasoulas, A. Filippoupolitis, A. (1997). Οι Ελληνικοί Σιδηρόδρομοι (Hellenic Railways) (in Greek). Μίλητος (Militos). ISBN 960-8460-07-7.
- Simms, W.F. (1997). The railways of Greece. Wilfried F. Sims. ISBN 0-9528881-1-4.