The museum documents the culture and history of people of African descent (most slaves, some free, and not all Americans) in northeast Florida prior to that territory's entry as a U.S. state in 1845, as well as LaVilla neighborhood of downtown Jacksonville (which was once a large and thriving African American community). LaVilla was home to so many poets, artists, musicians, authors, and playwrights that it was known as "the Harlem of the South". The Ritz Theatre is one of the few remaining buildings in the LaVilla neighborhood. Although most of the 600-seat theatre was razed in the 1990s, the northwest corner is original to the building.
The highlight of the museum tour are two animatronic representations of James Weldon Johnson and J. Rosamond Johnson, LaVilla natives who composed the famous American Civil Rights Movement song, Lift Every Voice and Sing. Rooms in the building evoke African American life throughout the 20th century by recreating a typical home living room, a Christian church, a barber shop, and a school room.
- Bull, Roger. "Ritz Theatre Celebrates 10 Years Back in Business in LaVilla." Florida Times-Union. September 14, 2009. Accessed 2012-03-03.
- Carbone, Marisa. Insiders' Guide to Jacksonville. Guilford, Con..: Globe Pequot Press, 2003, p. 101.
- Hurst, Rodney L. It Was Never About a Hot Dog and a Coke!: A Personal Account of the 1960 Sit-In Demonstrations in Jacksonville, Florida and Ax Handle Saturday. Livermore, Calif.: WingSpan Press, 2008, p. 16.
- Cobb, Jr., Charles E. On the Road to Freedom: A Guided Tour of the Civil Rights Trail. Chapel Hill, N.C.: Algonquin Books of Chapel Hill, 2008, p. 353.
- Abravanel, Lesley and Miller, Laura Lea. Frommer's Florida 2010. Hoboken, N.J.: Wiley Publishing, 2009, p. 554.
- Carrier, Jim. A Traveler's Guide to the Civil Rights Movement. Orlando, Fla.: Harcourt, 2004, p. 191.