In America Lady Jane was the B-side to "Mother's Little Helper", but "Lady Jane" reached #24 on Billboard's Hot 100 singles chart. "Mother's Little Helper" reached # 8, making the release one of the few singles with both songs becoming hits in the US.
It has an Elizabethan atmosphere with its lyrics and Brian Jones'sdulcimer, without any drums on the studio version. However, live recording have drums included. The song was a part of their live act during their 1966 and 1967 tours. They also performed the song during their fourth TV appearance on The Ed Sullivan Show. Since Jones's death in 1969, the Rolling Stones had not played the song, but it was included in the setlist for the band's second 50th anniversary show at the O2 Arena in London in November 2012. It was originally included in the first night's set but dropped for an unknown reason. However it was played during the second night for the first time since 1967, Jagger mentioned that it was the first time they'd played it in a long time in public, implying the band had rehearsed it over the years.