The Langendorff heart or isolated perfused heart assay is a predominant in vitro technique used in pharmacological and physiological research using animals. It allows the examination of cardiac contractile strength and heart rate without the complications of an intact animal.
In the Langendorff preparation, the heart is removed from the animal's body, severing the blood vessels; it is then perfused in a reverse fashion via the aorta, usually with a nutrient rich, oxygenated solution (e.g. Krebs–Henseleit solution or Tyrode's solution). The backwards pressure causes the aortic valve to shut, forcing the solution into the coronary vessels, which normally supply the heart tissue with blood. This feeds nutrients and oxygen to the cardiac muscle, allowing it to continue beating for several hours after its removal from the animal. This is a useful preparation because it allows the addition of drugs (via the perfusate) and observation of their effect on the heart without the complications involved with in vivo experimentation, such as neuronal and hormonal effects from living animal.
- The Langendorff heart preparation—Reappraisal of its role as a research and teaching model for coronary vasoactive drugs, K.J. Broadley, Journal of Pharmacological Methods
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