Lankershim Boulevard starts off at San Fernando Road in the Sun Valley portion of the San Fernando Valley. In addition to Sun Valley, it runs through North Hollywood and Universal City. It runs for about 7.3 miles (11.8 kilometers) before ending directly south of Ventura Boulevard. It crosses intersection with Interstate 5, State Route 134, and US Route 101.
Metro Local line 224 serves Lankershim Boulevard. It is also briefly served by Metro Local lines 154, 162, and 353. It passes two Metro Rail stations, North Hollywood and Universal City. Both of which are served by the Red Line, and the North Hollywood Station is also served by the Orange Line.