The genus Legionella is a pathogenic group of gram negative bacteria, that includes the species L. pneumophila, causing Legionellosis  (all illnesses caused by Legionella) including a pneumonia type illness called Legionnaires Disease and a mild flu like illness called Pontiac fever.
The side-chains of the cell wall carry the bases responsible for the somatic antigen specificity of these organisms. The chemical composition of these side chains both with respect to components as well as arrangement of the different sugars determines the nature of the somatic or O antigen determinants, which are essential means of serologically classifying many Gram-negative bacteria.
Legionella acquired its name after a July 1976 outbreak of a then-unknown "mystery disease" sickened 221 persons, causing 34 deaths. The outbreak was first noticed among people attending a convention of the American Legion—an association of U.S. military veterans. The convention in question occurred in Philadelphia during the U.S. Bicentennial year in July 21–24, 1976. This epidemic among U.S. war veterans, occurring in the same city as—and within days of the 200th anniversary of—the signing of the Declaration of Independence, was widely publicized and caused great concern in the United States. On January 18, 1977, the causative agent was identified as a previously unknown bacterium subsequently named Legionella. See Legionnaires' Disease for full details.
- 1 Detection
- 2 Pathogenesis
- 3 Molecular biology
- 4 Legionella control
- 5 Weaponization
- 6 See also
- 7 References
- 8 External links
Legionella is traditionally detected by culture on buffered charcoal yeast extract (BCYE) agar. Legionella requires the presence of cysteine and iron to grow and therefore does not grow on common blood agar media used for laboratory based total viable counts or on site dipslides. Common laboratory procedures for the detection of Legionella in water concentrate the bacteria (by centrifugation and/or filtration through 0.2 micrometre filters) before inoculation onto a charcoal yeast extract agar containing antibiotics (e.g. glycine vancomycim polymixin cyclohexamide, GVPC) to suppress other flora in the sample. Heat or acid treatment are also used to reduce interference from other microbes in the sample.
After incubation for up to 10 days, suspect colonies are confirmed as Legionella if they grow on BCYE containing cysteine, but not on agar without cysteine added. Immunological techniques are then commonly used to establish the species and/or serogroups of bacteria present in the sample.
Although the plating method is quite specific for most species of Legionella, one study has shown that a coculture method that accounts for the close relationship with amoebas may be more sensitive since it can detect the presence of the bacteria even when masked by its presence inside the amoeba. Consequently, the true clinical and environmental prevalence of the bacteria is likely to be underestimated due to false negatives inherent in the current lab methodology.
Many hospitals use the Legionella Urinary Antigen test for initial detection when Legionella pneumonia is suspected. Some of the advantages offered by this test is that the results can be obtained in a matter of hours rather than the five days required for culture, and that a urine specimen is generally more easily obtained than a sputum specimen. Disadvantages are that the urine antigen test only detects antigen of Legionella pneumophila serogroup 1 (LP1); only a culture will detect infection by non-LP1 strains or other Legionella species and that isolates of Legionella are not obtained, which impairs public health investigations of outbreaks of LD.
New techniques for the rapid detection of Legionella in water samples are emerging including the use of polymerase chain reaction (PCR) and rapid immunological assays. These technologies can typically provide much faster results.
Legionella live within amoebae in the natural environment.
Upon inhalation the bacteria can infect alveolar macrophages. subverting the normal host cell machinery to create a niche where the bacteria can replicate. This results in Legionnaires' disease and the lesser form, Pontiac fever. Legionella transmission is airborne via respiratory droplets containing the bacteria. Once inside a host, incubation may take up to two weeks. Prodromal symptoms are flu-like, including fever, chills, and dry cough. Advanced stages of the disease cause problems with the gastrointestinal tract and the nervous system and lead to diarrhea and nausea. Other advanced symptoms of pneumonia may also present.
However, the disease is generally not a threat to most healthy individuals, and tends to lead to harmful symptoms only in those with a compromised immune system and the elderly. (INCORRECT). Consequently, it should be actively checked for in the water systems of hospitals and nursing homes. The Texas Department of State Health services provides recommendations for hospitals to detect and prevent the spread of nosocomial infection (hospital acquired disease) due to legionella. According to the journal "Infection Control and Hospital Epidemiology," Hospital-acquired Legionella pneumonia has a fatality rate of 28%, and the source is the water distribution system.
In the United States, the disease affects between 8,000 to 18,000 individuals a year.
Person-to-person transmission of Legionella has not been demonstrated.
Legionella species typically exist in nature at low concentrations, it has been found in groundwater, lakes, and streams. After entering manmade equipment, given the right environmental conditions, it may reproduce.
Sources of Legionella
Documented sources include cooling towers, swimming pools (especially in Scandinavian countries), domestic hot-water systems and showers, ice making machine, refrigerated cabinets, whirlpool spas, hot springs, fountains, dental equipment and industrial coolant.
Airborne Transmission from Cooling Towers
The largest and most common source of Legionnaires' disease outbreaks are cooling towers (heat rejection equipment used in air conditioning and industrial cooling water systems) primarily because of the risk for wide spread circulation. Many governmental agencies, cooling tower manufacturers, and industrial trade organisations have developed design and maintenance guidelines for controlling the growth and proliferation of Legionella within cooling towers.
Recent research in the Journal of Infectious Diseases provides evidence that Legionella pneumophila, the causative agent of Legionnaires' disease, can travel at least 6 km from its source by airborne spread. It was previously believed that transmission of the bacterium was restricted to much shorter distances. A team of French scientists reviewed the details of an epidemic of Legionnaires' disease that took place in Pas-de-Calais, northern France, in 2003–2004. There were 86 confirmed cases during the outbreak, of which 18 resulted in death. The source of infection was identified as a cooling tower in a petrochemical plant, and an analysis of those affected in the outbreak revealed that some infected people lived as far as 6–7 km from the plant.
There is no vaccine for legionellosis, and antibiotic prophylaxis is not effective. Any licensed vaccine for humans in the US is most probably still many years away. Vaccination studies using heat-killed or acetone-killed cells have been carried out, and guinea pigs were challenged intraperitoneally or by using the aerosol model of infection. Both vaccines were shown to give moderately high levels of protection. Protection was found to be dose dependent and correlated with antibody levels as measured by enzyme-linked immunosorbent assay to an outer membrane antigen and by indirect immunofluorescence to heat-killed cells.
It has been discovered that Legionella is a genetically diverse species with 7-11% of genes strain specific. The molecular function of some of the proven virulence factors of Legionella have been discovered by some researchers.
Control of Legionella growth can occur through chemical or thermal methods. The most expensive of these two options is temperature control—i.e., keeping all cold water below 25 °C (78 °F) and all hot water above 51 °C (124 °F). The high cost incurred with this method arises from the extensive retrofitting required for existing complex distribution systems in large facilities and the energy cost of chilling or heating the water and maintaining the required temperatures at all times and at all distal points within the system.
- Above 70 °C (158 °F) - Legionella dies almost instantly
- At 60 °C (140 °F) - 90% die in 2 minutes (Decimal reduction time (D) = 2)
- At 50 °C (122 °F) - 90% die in 80–124 minutes, depending on strain (Decimal reduction time (D) = 80-124)
- 48 to 50 °C (118 to 122 °F) - Can survive but do not multiply
- 32 to 42 °C (90 to 108 °F) - Ideal growth range
- 25 to 45 °C (77 to 113 °F) - Growth range
- Below 20 °C (68 °F) - Can survive but are dormant, even below freezing
- 60 to 70 °C (140 to 158 °F) to 80 °C (176 °F) - Disinfection range
- 66 °C (151 °F) - Legionella die within 2 minutes
- 60 °C (140 °F) - Legionella die within 32 minutes
- 55 °C (131 °F) - Legionella die within 5 to 6 hours
- 20 °C (68 °F) to 45 °C (113 °F) - Legionella multiply
- 20 °C (68 °F) & below - Legionella are dormant
A very effective chemical treatment is chlorine. For systems with marginal issues chlorine will provide effective results at 0.5 ppm residual in the hot water system. For systems with significant Legionella problems, temporary shock chlorination—where levels are raised to higher than 2 ppm for a period of 24-hours or more and then returned to 0.5 ppm—may be effective (NEED REFERENCE). Hyper-chlorination can also be used where the water system is taken out of service and the chlorine residual is raised to 50 to 100 ppm or higher at all distal points for 24-hours or more. The system is then flushed and returned to 0.5 ppm chlorine prior to being placed back into service. These high levels of chlorine will penetrate biofilm killing both the Legionella bacteria and the host organisms. Annual hyper-chlorination can be an effective part of a comprehensive legionella prevention action plan.
Copper-silver (CuAg) ionization
Industrial-size copper-silver ionization is recognized by the U.S. Environmental Protection Agency and WHO for Legionella control and prevention. <NEED REFERENCE> Copper and silver ion concentrations must be maintained at optimal levels, taking into account both water flow and overall water usage, to control Legionella. The disinfection function within all of a facilities water distribution network will occur within 30 to 45 days <NEED REFERENCE>. Key engineering features such as 10 amps per ion chamber cell and automated variable voltage outputs having no less than 0–100 VDC are but a few of the required features for proper Legionella control and prevention, using a specific, non-referenced CuAg system. Swimming pool ion generators are not designed for potable water treatment.
Questions remain whether the silver and copper ion concentrations required for effective control of symbiotic hosts could exceed those allowed under the U.S. Safe Drinking Water Act's Lead and Copper Rule. In any case, any facility or public water system using CuAg for disinfection should monitor their copper and silver ion concentrations to ensure that they are within intended levels - both minimum and maximum. Further, there are no current standards for silver in the EU and other regions utilizing this technology.
CuAg ionization is an effective process to control Legionella in potable water distribution systems found in health facilities, hotels, nursing homes and most large buildings. CuAg is not intended for cooling towers because of pH levels over 8.6 that cause ionic copper to precipitate. In 2003, researchers who heavily support ionization developed a four (four whats?) validation process that supports their research on ionization. Ionization became the first such hospital disinfection process to have fulfilled a proposed four-step modality evaluation; by then it had been adopted by over 100 hospitals. Additional studies indicate ionization is superior to thermal eradication. 
Chlorine dioxide has been EPA approved as a primary potable water disinfectant since 1945. It does not produce any carcinogenic byproducts like chlorine and is not a restricted heavy metal like copper. It has proven excellent control of Legionella in cold and hot water systems and its ability as a biocide is not impacted by pH, or any water corrosion inhibitors like silica or phosphate. Monochloramine is an alternative. Like chlorine and chlorine dioxide, monochloramine is EPA approved as a primary potable water disinfectant. EPA registration requires an EPA biocide label which lists toxicity and other data required by the EPA for all EPA registered biocides. If the product is being sold as a biocide then the manufacturer is legally required to supply a biocide label. And the purcharser is legally required to apply the biocide per the biocide label. When first applied to a system chlorine dioxide can be added at disinfection levels of 2 ppm for 6 hours to clean up a system. This will not remove all biofilm but will effectively remediate the system of Legionella.
Moist heat sterilization
Several European countries established the European Working Group for Legionella Infections (EWGLI) to share knowledge and experience about monitoring potential sources of Legionella. The EWGLI has published guidelines about the actions to be taken to limit the number of colony-forming units (CFU, that is, live bacteria that are able to multiply) of Legionella per litre:
|Legionella bacteria CFU/litre||Action required (35 samples per facility are required, including 20 water and 10 swabs)|
|1000 or less||System under control.|
|more than 1000
up to 10,000
|Review program operation. The count should be confirmed by immediate re-sampling. If a similar count is found again, a review of the control measures and risk assessment should be carried out to identify any remedial actions.|
|more than 10,000||Implement corrective action. The system should immediately be re-sampled. It should then be "shot dosed" with an appropriate biocide, as a precaution. The risk assessment and control measures should be reviewed to identify remedial actions. (150+ CFU/ml in healthcare facilities or nursing homes require immediate action.)|
Minimal monitoring guidelines are stated in ACOP L8 in the UK. These are not mandatory however are widely regarded as so. An ACOP is an Approved Code of Practice which an employer or property owner must follow, or achieve the same result. Failure to show monitoring records to at least this standard has resulted in several high profile prosecutions, e.g. Nalco + Bulmers - Both could not prove a sufficient scheme to be in place whilst investigating an outbreak, therefore both were fined in the region of £300,000GBP. Important case law in this area is R v Trustees of the Science Museum 3 All ER 853, (1993) 1 WLR 1171
Any building within the UK which is subject to HASAW 1974 is required under COSHH and ACOP L8 to have a legionella risk assessment carried out. The report should include a detailed narrative of the site, asset register, simplified schematic drawings (if none available on site), recommendations on compliance and a proposed monitoring scheme.
Log books should be held on site for a minimum of 5 years. E-logbooks are available, however issues can arise if a site audit is carried out and the auditor cannot access the server for any reason (User isn't set up, someone is on holiday/ill, etc.). Electronic logbooks are generally more useful when managing large portfolios, however a duplication is advisable because of the 5 year 'on site / available for inspection' requirement, and therefore kills the 'no paper' argument.
The requirements of the L8 ACOP and regulations says that the legionnaires risk assessment should be reviewed at least every 2 years and whenever there is reason to suspect it is no longer valid, such as if you have added to, or modified, your water systems, or if the use of the water system has changed, or if your Legionella control measures are no longer working.
It has been suggested that Legionella could be used as a weapon and indeed genetic modification of Legionella pneumophila has been shown where the mortality rate in infected animals can be increased to nearly 100%.
- Copper-silver ionization
- Environmental microbiology
- European Working Group for Legionella Infections
- Nosocomial infection
- Legionella and the prevention of legionellosis.
- Lawrence K. Altman (August 1, 2006). "In Philadelphia 30 Years Ago, an Eruption of Illness and Fear". New York Times.
- ISO 11731-2:2004 Water quality -- Detection and enumeration of Legionella -- Part 2: Direct membrane filtration method for waters with low bacterial counts
- La Scola B, Mezi L, Weiller PJ, and Raoult1 D (2001). "Isolation of Legionella anisa Using an Amoebic Coculture Procedure". J Clin Microbiol. 39(1): 365–6. doi:10.1128/JCM.39.1.365-366.2001. Retrieved 2013-06-28.
- Trends in legionnaires disease, 1980-1998: declining mortality and new patterns of diagnosis. Benin AL; Benson RF; Besser RE. Clin Infect Dis November 1, 2002;35(9):1039-46. Epub October 14, 2002.
- Swanson M, Hammer B (2000). "Legionella pneumophila pathogesesis: a fateful journey from amoebae to macrophages". Annu Rev Microbiol 54: 567–613. doi:10.1146/annurev.micro.54.1.567. PMID 11018138.
- Report of the Texas Legionnaires' Disease Task Force, Texas Department of State Health Services 
- Infection Control and Hospital Epidemiology, July 2007, Vol. 28, No. 7, "Role of Environmental Surveillance in Determining the Risk of Hospital-Acquired Legionellosis: A National Surveillance Study With Clinical Correlations" 
- Winn, W.C. Jr. (1996). Legionella (In: Baron's Medical Microbiology, Baron, S. et al., eds. (4th ed.). University of Texas Medical Branch. ISBN 0-9631172-1-1. (via NCBI Bookshelf)
- Nguyen, T.; Ilef, D.; Jarraud, S.; Rouil, L.; Campese, C.; Che, D.; Haeghebaert, S.; Ganiayre, F.; Marcel, F.; Etienne, J.; Desenclos, J. (2006). "A community-wide outbreak of legionnaires disease linked to industrial cooling towers—how far can contaminated aerosols spread?". Journal of Infectious Diseases 193 (1): 102–11. doi:10.1086/498575. PMID 16323138.
- Raychaudhury S, Farelli JD, Montminy TP, Matthews M, Ménétret JF, Duménil G, Roy CR, Head JF, Isberg RR, Akey CW (Apr 2009). "Structure and function of interacting IcmR-IcmQ domains from a Type IVb secretion system in Legionella pneumophila". Structure 17 (4): 590–601. doi:10.1016/j.str.2009.02.011. PMC 2693034. PMID 19368892.
- "LEGIONELLA and the prevention of legionellosis".
- "Safe Hot Water Temperature".
- "Controlling Legionella in Domestic Hot Water Systems".
- "Employers Guide to the control of Legionella".
- Stout & Yu 2003 "(1) Demonstrated efficacy of Legionella eradication in vitro using laboratory assays, (2) anecdotal experiences in preventing legionnaires’ disease in individual hospitals, (3) controlled studies in individual hospitals, and (4) validation in confirmatory reports from multiple hospitals during a prolonged time."
- Block 2001.
- "European Working Group for Legionella Infections".
- Gilsdorf et al., Clinical Infectious Diseases 2005; 40 p1160–1165 "New Considerations in Infectious Disease Outbreaks: The Threat of Genetically Modified Microbes" http://cid.oxfordjournals.org/content/40/8/1160.full
LEGIONELLA and the prevention of legionellosis 
- CDC Division of bacterial and Mycotic Diseases: Legionellosis
- Directors of Health Promotion and Education page on Legionellosis
- European Working Group for Legionella Infections
- Legionnaires' disease outbreaks
- Legionella Information
- Legionella Risk management
- Legionella case studies
- Centers for Disease Control and Prevention - Procedure for Cleaning Cooling Towers and Related Equipment (pages 239 and 240 of 249)
- Cooling Technology Institute - Best Practices for Control of Legionella
- California Energy Commission - Cooling Water Management Program Guidelines For Wet and Hybrid Cooling Towers at Power Plants
- ASHRAE Guideline
- Guidelines for Control of Legionella in Ornamental Fountains
- Employers Guidelines for prevention of Legionella