The Lexington History Center is host to three museums:
The Lexington History Museum showcases numerous exhibits regarding Lexington's history, and offers numerous programs tailored to all ages. Following the closure of the history center, the Lexington History Museum utilized empty spaces in downtown and created pocket museums to house its exhibits.
The Lexington Public Safety Museum opened on November 20, 2004. The museum features exhibits that explore the history of Lexington's fire and police departments.
The Kentucky Renaissance Pharmacy Museum is dedicated to the early history of Bluegrass region pharmacies.