"Your Life's on the Line" is included on 50 Cent's first two studio albums: it appears as track 5 on his unofficially released 2000 debut album Power of the Dollar and, as a bonus track, as track 19 on his 2003 commercial debut album Get Rich or Die Tryin'. In his review of Power of the Dollar, Jason Birchmeier of AllMusic states that the song "conveys street life through hard, violent rhythms".
The music videos for "Your Life's on the Line" and the In Too Deep soundtrack single "Rowdy Rowdy" were produced prior to 50 Cent's 2000 shooting, which preceded the planned filming of the music video for "Thug Love" by two days. The music video starts with a statement explaining how the footage went missing, but was later found, then proceeds with scenes of 50 Cent rapping in various areas of New York City, getting arrested, and rapping while in the police car. The video features cameos from rappers Tony Yayo, Bang 'Em Smurf and Two Five.