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Ligation may refer to:
- Ligation (molecular biology), the covalent linking of two ends of DNA or RNA molecules, most commonly done using DNA ligase, RNA ligase (ATP) or other enzymes.
- In medicine, the making of a ligature (tie)
- Chemical ligation, the production of peptides from amino acids
- Tubal ligation, a method of female sterilization
- Rubber band ligation, a treatment for hemorrhoids
- In Coordination chemistry, making a bond between a ligand and a Lewis acid
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