The lipid bilayer is a thin polar membrane made of two layers of lipid molecules. These membranes are flat sheets that form a continuous barrier around cells. The cell membrane of almost all living organisms and many viruses are made of a lipid bilayer, as are the membranes surrounding the cell nucleus and other sub-cellular structures. The lipid bilayer is the barrier that keeps ions, proteins and other molecules where they are needed and prevents them from diffusing into areas where they should not be. Lipid bilayers are ideally suited to this role because, even though they are only a few nanometers in width, they are impermeable to most water-soluble (hydrophilic) molecules. Bilayers are particularly impermeable to ions, which allows cells to regulate salt concentrations and pH by pumping ions across their membranes using proteins called ion pumps.
Natural bilayers are usually composed of phospholipids, which have a hydrophilic head and two hydrophobic tails each. When phospholipids are exposed to water, they arrange themselves into a two-layered sheet (a bilayer) with all of their tails pointing toward the center of the sheet. The center of this bilayer contains almost no water and excludes molecules like sugars or salts that dissolve in water but not in oil. This assembly process is similar to the coalescing of oil droplets in water and is driven by the same force, called the hydrophobic effect. Because lipid bilayers are quite fragile and are so thin that they are invisible in a traditional microscope, bilayers are very challenging to study. Experiments on bilayers often require advanced techniques like electron microscopy and atomic force microscopy.
Phospholipids with certain head groups can alter the surface chemistry of a bilayer and can, for example, mark a cell for destruction by the immune system. Lipid tails can also affect membrane properties, for instance by determining the phase of the bilayer. The bilayer can adopt a solid gel phase state at lower temperatures but undergo phase transition to a fluid state at higher temperatures. The packing of lipids within the bilayer also affects its mechanical properties, including its resistance to stretching and bending. Many of these properties have been studied with the use of artificial "model" bilayers produced in a lab. Vesicles made by model bilayers have also been used clinically to deliver drugs.
Biological membranes typically include several types of lipids other than phospholipids. A particularly important example in animal cells is cholesterol, which helps strengthen the bilayer and decrease its permeability. Cholesterol also helps regulate the activity of certain integral membrane proteins. Integral membrane proteins function when incorporated into a lipid bilayer. Because bilayers define the boundaries of the cell and its compartments, these membrane proteins are involved in many intra- and inter-cellular signaling processes. Certain kinds of membrane proteins are involved in the process of fusing two bilayers together. This fusion allows the joining of two distinct structures as in the fertilization of an egg by sperm or the entry of a virus into a cell.
- 1 Structure and organization
- 2 Biological roles
- 3 Characterization methods
- 4 Transport across the bilayer
- 5 Mechanics
- 6 Fusion
- 7 Model systems
- 8 Commercial applications
- 9 History
- 10 See also
- 11 References
- 12 External links
Structure and organization
A lipid bilayer, also known as the phospholipid bilayer, is a sheet of lipids two molecules thick, arranged so that the hydrophilic phosphate heads point “out” to the water on either side of the bilayer and the hydrophobic tails point “in” to the core of the bilayer. This arrangement results in two “leaflets” that are each a single molecular layer. Lipids self-assemble into this structure because of the hydrophobic effect, which creates an energetically unfavorable interaction between the hydrophobic lipid tails and the surrounding water. Thus, a lipid bilayer is typically held together by entirely non-covalent forces that do not involve formation of chemical bonds between individual molecules.
There are a few similarities between this structure and a common soap bubble, although there are also important differences. As illustrated, both structures involve two single-molecule layers of an amphiphilic substance. In the case of a soap bubble, the two soap monolayers coat an intervening water layer. The hydrophilic heads are oriented “in” toward this water core, while the hydrophobic tails point “out” to the air. In the case of a lipid bilayer, this structure is reversed with heads out and tails in. Another important difference between lipid bilayers and soap bubbles is their relative size. Soap bubbles are typically hundreds of nanometers thick, on the same order as the wavelength of light, which is why interference effects cause rainbow colors on a bubble surface. A single lipid bilayer, on the other hand, is around five nanometers thick, much smaller than the wavelength of light and is therefore invisible to the eye, even with a standard light microscope.
Cross section analysis
The lipid bilayer is very thin compared to its lateral dimensions. If a typical mammalian cell (diameter ~10 micrometre) were magnified to the size of a watermelon (~1 ft/30 cm), the lipid bilayer making up the plasma membrane would be about as thick as a piece of office paper. Despite being only a few nanometers thick, the bilayer is composed of several distinct chemical regions across its cross-section. These regions and their interactions with the surrounding water have been characterized over the past several decades with x-ray reflectometry, neutron scattering and nuclear magnetic resonance techniques.
The first region on either side of the bilayer is the hydrophilic headgroup. This portion of the membrane is completely hydrated and is typically around 0.8-0.9 nm thick. In phospholipid bilayers the phosphate group is located within this hydrated region, approximately 0.5 nm outside the hydrophobic core. In some cases, the hydrated region can extend much further, for instance in lipids with a large protein or long sugar chain grafted to the head. One common example of such a modification in nature is the lipopolysaccharide coat on a bacterial outer membrane, which helps retain a water layer around the bacterium to prevent dehydration.
Next to the hydrated region is an intermediate region that is only partially hydrated. This boundary layer is approximately 0.3 nm thick. Within this short distance, the water concentration drops from 2M on the headgroup side to nearly zero on the tail (core) side. The hydrophobic core of the bilayer is typically 3-4 nm thick, but this value varies with chain length and chemistry. Core thickness also varies significantly with temperature, in particular near a phase transition.
In many naturally occurring bilayers, the compositions of the inner and outer membrane leaflets are different. In human red blood cells, the inner (cytoplasmic) leaflet is composed mostly of phosphatidylethanolamine, phosphatidylserine and phosphatidylinositol and its phosphorylated derivatives. By contrast, the outer (extracellular) leaflet is based on phosphatidylcholine, sphingomyelin and a variety of glycolipids, In some cases, this asymmetry is based on where the lipids are made in the cell and reflects their initial orientation. The biological functions of lipid asymmetry are imperfectly understood, although it is clear that it is used in several different situations. For example, when a cell undergoes apoptosis, the phosphatidylserine — normally localised to the cytoplasmic leaflet — is transferred to the outer surface: There, it is recognised by a macrophage that then actively scavenges the dying cell.
Lipid asymmetry arises, at least in part, from the fact that most phospholipids are synthesised and initially inserted into the inner monolayer: those that constitute the outer monolayer are then transported from the inner monolayer by a class of enzymes called flippases. Other lipids, such as sphingomyelin, appear to be synthesised at the external leaflet. Flippases are members of a larger family of lipid transport molecules that also includes floppases, which transfer lipids in the opposite direction, and scramblases, which randomize lipid distribution across lipid bilayers (as in apoptotic cells). In any case, once lipid asymmetry is established, it does not normally dissipate quickly because spontaneous flip-flop of lipids between leaflets is extremely slow.
It is possible to mimic this asymmetry in the laboratory in model bilayer systems. Certain types of very small artificial vesicle will automatically make themselves slightly asymmetric, although the mechanism by which this asymmetry is generated is very different from that in cells. By utilizing two different monolayers in Langmuir-Blodgett deposition or a combination of Langmuir-Blodgett and vesicle rupture deposition it is also possible to synthesize an asymmetric planar bilayer. This asymmetry may be lost over time as lipids in supported bilayers can be prone to flip-flop.
Phases and phase transitions
At a given temperature a lipid bilayer can exist in either a liquid or a gel (solid) phase. All lipids have a characteristic temperature at which they transition (melt) from the gel to liquid phase. In both phases the lipid molecules are prevented from flip-flopping across the bilayer, but in liquid phase bilayers a given lipid will exchange locations with its neighbor millions of times a second. This random walk exchange allows lipid to diffuse and thus wander across the surface of the membrane. Unlike liquid phase bilayers, the lipids in a gel phase bilayer are locked in place.
The phase behavior of lipid bilayers is determined largely by the strength of the attractive Van der Waals interactions between adjacent lipid molecules. Longer-tailed lipids have more area over which to interact, increasing the strength of this interaction and, as a consequence, decreasing the lipid mobility. Thus, at a given temperature, a short-tailed lipid will be more fluid than an otherwise identical long-tailed lipid. Transition temperature can also be affected by the degree of unsaturation of the lipid tails. An unsaturated double bond can produce a kink in the alkane chain, disrupting the lipid packing. This disruption creates extra free space within the bilayer that allows additional flexibility in the adjacent chains. An example of this effect can be noted in everyday life as butter, which has a large percentage saturated fats, is solid at room temperature while vegetable oil, which is mostly unsaturated, is liquid.
Most natural membranes are a complex mixture of different lipid molecules. If some of the components are liquid at a given temperature while others are in the gel phase, the two phases can coexist in spatially separated regions, rather like an iceberg floating in the ocean. This phase separation plays a critical role in biochemical phenomena because membrane components such as proteins can partition into one or the other phase and thus be locally concentrated or activated. One particularly important component of many mixed phase systems is cholesterol, which modulates bilayer permeability, mechanical strength, and biochemical interactions.
While lipid tails primarily modulate bilayer phase behavior, it is the headgroup that determines the bilayer surface chemistry. Most natural bilayers are composed primarily of phospholipids, although sphingolipids such as sphingomyelin and sterols such as cholesterol are also important components. Of the phospholipids, the most common headgroup is phosphatidylcholine (PC), accounting for about half the phospholipids in most mammalian cells. PC is a zwitterionic headgroup, as it has a negative charge on the phosphate group and a positive charge on the amine but, because these local charges balance, no net charge.
Other headgroups are also present to varying degrees and can include phosphatidylserine (PS) phosphatidylethanolamine (PE) and phosphatidylglycerol (PG). These alternate headgroups often confer specific biological functionality that is highly context-dependent. For instance, PS presence on the extracellular membrane face of erythrocytes is a marker of cell apoptosis, whereas PS in growth plate vesicles is necessary for the nucleation of hydroxyapatite crystals and subsequent bone mineralization. Unlike PC, some of the other headgroups carry a net charge, which can alter the electrostatic interactions of small molecules with the bilayer.
Containment and separation
The primary role of the lipid bilayer in biology is to separate aqueous compartments from their surroundings. Without some form of barrier delineating “self” from “non-self,” it is difficult to even define the concept of an organism or of life. This barrier takes the form of a lipid bilayer in all known life forms except for a few species of archaea that utilize a specially adapted lipid monolayer. It has even been proposed that the very first form of life may have been a simple lipid vesicle with virtually its sole biosynthetic capability being the production of more phospholipids. The partitioning ability of the lipid bilayer is based on the fact that hydrophilic molecules cannot easily cross the hydrophobic bilayer core, as discussed in Transport across the bilayer below. Nucleus, mitochondria and chloroplasts have two lipid bilayer, and other structures are surrounded by a single lipid bilayer (such as the plasma membrane, endoplasmic reticula, Golgi apparatuses and lysosomes). See Organelle.
Prokaryotes have only one lipid bilayer- the cell membrane (also known as the plasma membrane). Many prokaryotes also have a cell wall, but the cell wall is composed of proteins or long chain carbohydrates, not lipids. In contrast, eukaryotes have a range of organelles including the nucleus, mitochondria, lysosomes and endoplasmic reticulum. All of these sub-cellular compartments are surrounded by one or more lipid bilayers and, together, typically comprise the majority of the bilayer area present in the cell. In liver hepatocytes for example, the plasma membrane accounts for only two percent of the total bilayer area of the cell, whereas the endoplasmic reticulum contains more than fifty percent and the mitochondria a further thirty percent.
Probably the most familiar form of cellular signaling is synaptic transmission, whereby a nerve impulse that has reached the end of one neuron is conveyed to an adjacent neuron via the release of neurotransmitters. This transmission is made possible by the action of synaptic vesicles loaded with the neurotransmitters to be released. These vesicles fuse with the cell membrane at the pre-synaptic terminal and release its contents to the exterior of the cell. The contents then diffuse across the synapse to the post-synaptic terminal.
Lipid bilayers are also involved in signal transduction through their role as the home of integral membrane proteins. This is an extremely broad and important class of biomolecule. It is estimated that up to a third of the human proteome may be membrane proteins. Some of these proteins are linked to the exterior of the cell membrane. An example of this is the CD59 protein, which identifies cells as “self” and thus inhibits their destruction by the immune system. The HIV virus evades the immune system in part by grafting these proteins from the host membrane onto its own surface. Alternatively, some membrane proteins penetrate all the way through the bilayer and serve to relay individual signal events from the outside to the inside of the cell. The most common class of this type of protein is the G protein-coupled receptor (GPCR). GPCRs are responsible for much of the cell’s ability to sense its surroundings and, because of this important role, approximately 40% of all modern drugs are targeted at GPCRs.
In addition to protein- and solution-mediated processes, it is also possible for lipid bilayers to participate directly in signaling. A classic example of this is phosphatidylserine-triggered phagocytosis. Normally, phosphatidylserine is asymmetrically distributed in the cell membrane and is present only on the interior side. During programmed cell death a protein called a scramblase equilibrates this distribution, displaying phosphatidylserine on the extracellular bilayer face. The presence of phosphatidylserine then triggers phagocytosis to remove the dead or dying cell.
The lipid bilayer is a very difficult structure to study because it is so thin and fragile. In spite of these limitations dozens of techniques have been developed over the last seventy years to allow investigations of its structure and function.
Electrical measurements are a straightforward way to characterize an important function of a bilayer: its ability to segregate and prevent the flow of ions in solution. By applying a voltage across the bilayer and measuring the resulting current, the resistance of the bilayer is determined. This resistance is typically quite high since the hydrophobic core is impermeable to charged species. The presence of even a few nanometer-scale holes results in a dramatic increase in current. The sensitivity of this system is such that even the activity of single ion channels can be resolved.
Electrical measurements do not provide an actual picture like imaging with a microscope can. Lipid bilayers cannot be seen in a traditional microscope because they are too thin. In order to see bilayers, researchers often use fluorescence microscopy. A sample is excited with one wavelength of light and observed in a different wavelength, so that only fluorescent molecules with a matching excitation and emission profile will be seen. Natural lipid bilayers are not fluorescent, so a dye is used that attaches to the desired molecules in the bilayer. Resolution is usually limited to a few hundred nanometers, much smaller than a typical cell but much larger than the thickness of a lipid bilayer.
Electron microscopy offers a higher resolution image. In an electron microscope, a beam of focused electrons interacts with the sample rather than a beam of light as in traditional microscopy. In conjunction with rapid freezing techniques, electron microscopy has also been used to study the mechanisms of inter- and intracellular transport, for instance in demonstrating that exocytotic vesicles are the means of chemical release at synapses.
31P-NMR(nuclear magnetic resonance) spectroscopy is widely used for studies of phospholipid bilayers and biological membranes in native conditions. The analysis of 31P-NMR spectra of lipids could provide a wide range of information about lipid bilayer packing, phase transitions (gel phase, physiological liquid crystal phase, ripple phases, non bilayer phases), lipid head group orientation/dynamics, and elastic properties of pure lipid bilayer and as a result of binding of proteins and other biomolecules.
In addition, a specific H-N...(O)-P NMR experiment (INEPT transfer by scalar coupling 3JH-P~5 Hz) could provide a direct information about formation of hydrogen bonds between amid protons of protein to phosphate of lipid headgroups, which is useful in studies of protein/membrane interactions.
A new method to study lipid bilayers is Atomic force microscopy (AFM). Rather than using a beam of light or particles, a very small sharpened tip scans the surface by making physical contact with the bilayer and moving across it, like a record player needle. AFM is a promising technique because it has the potential to image with nanometer resolution at room temperature and even under water or physiological buffer, conditions necessary for natural bilayer behavior. Utilizing this capability, AFM has been used to examine dynamic bilayer behavior including the formation of transmembrane pores (holes) and phase transitions in supported bilayers. Another advantage is that AFM does not require fluorescent or isotopic labeling of the lipids, since the probe tip interacts mechanically with the bilayer surface. Because of this, the same scan can image both lipids and associated proteins, sometimes even with single-molecule resolution. AFM can also probe the mechanical nature of lipid bilayers.
Lipid bilayers exhibit high levels of birefringence where the refractive index in the plane of the bilayer differs from that perpendicular by as much as 0.1 refractive index units. This has been used to characterise the degree of order and disruption in bilayers using dual polarisation interferometry to understand mechanisms of protein interaction.
Lipid bilayers are complicated molecular systems with many degrees of freedom. Thus atomistic simulation of membrane and in particular ab initio calculations of its properties is difficult and computationally expensive. Quantum chemical calculations has recently been successfully performed to estimate dipole and quadrupole moments of lipid membranes.
Hydrated bilayers show rich vibrational dynamics and are good media for efficient vibrational energy transfer. Vibrational properties of lipid monolayers and bilayers has been investigated by ultrafast spectroscopic techniques  and recently developed computational methods.
Transport across the bilayer
Most polar molecules have low solubility in the hydrocarbon core of a lipid bilayer and, as a consequence, have low permeability coefficients across the bilayer. This effect is particularly pronounced for charged species, which have even lower permeability coefficients than neutral polar molecules. Anions typically have a higher rate of diffusion through bilayers than cations. Compared to ions, water molecules actually have a relatively large permeability through the bilayer, as evidenced by osmotic swelling. When a cell or vesicle with a high interior salt concentration is placed in a solution with a low salt concentration it will swell and eventually burst. Such a result would not be observed unless water was able to pass through the bilayer with relative ease. The anomalously large permeability of water through bilayers is still not completely understood and continues to be the subject of active debate. Small uncharged apolar molecules diffuse through lipid bilayers many orders of magnitude faster than ions or water. This applies both to fats and organic solvents like chloroform and ether. Regardless of their polar character larger molecules diffuse more slowly across lipid bilayers than small molecules.
Ion pumps and channels
Two special classes of protein deal with the ionic gradients found across cellular and sub-cellular membranes in nature- ion channels and ion pumps. Both pumps and channels are integral membrane proteins that pass through the bilayer, but their roles are quite different. Ion pumps are the proteins that build and maintain the chemical gradients by utilizing an external energy source to move ions against the concentration gradient to an area of higher chemical potential. The energy source can be ATP, as is the case for the Na+-K+ ATPase. Alternatively, the energy source can be another chemical gradient already in place, as in the Ca2+/Na+ antiporter. It is through the action of ion pumps that cells are able to regulate pH via the pumping of protons.
In contrast to ion pumps, ion channels do not build chemical gradients but rather dissipate them in order to perform work or send a signal. Probably the most familiar and best studied example is the voltage-gated Na+ channel, which allows conduction of an action potential along neurons. All ion pumps have some sort of trigger or “gating” mechanism. In the previous example it was electrical bias, but other channels can be activated by binding a molecular agonist or through a conformational change in another nearby protein.
Endocytosis and exocytosis
Some molecules or particles are too large or too hydrophilic to pass through a lipid bilayer. Other molecules could pass through the bilayer but must be transported rapidly in such large numbers that channel-type transport is impractical. In both cases these types of cargo can be moved across the cell membrane through fusion or budding of vesicles. When a vesicle is produced inside the cell and fuses with the plasma membrane to release its contents into the extracellular space this process is known as exocytosis. In the reverse process a region of the cell membrane will dimple inwards and eventually pinch off, enclosing a portion of the extracellular fluid to transport it into the cell. Endocytosis and exocytosis rely on very different molecular machinery to function, but the two processes are intimately linked and could not work without each other. The primary mechanism this interdependence is the sheer volume of lipid material involved. In a typical cell, an area of bilayer equivalent to the entire plasma membrane will travel through the endocytosis/exocytosis cycle in about half an hour. If these two processes were not balancing each other the cell would either balloon outward to an unmanageable size or completely deplete its plasma membrane within a matter of minutes.
Electroporation is the rapid increase in bilayer permeability induced by the application of a large artificial electric field across the membrane. Experimentally, electroporation is used to introduce hydrophilic molecules into cells. It is a particularly useful technique for large highly charged molecules such as DNA, which would never passively diffuse across the hydrophobic bilayer core. Because of this, electroporation is one of the key methods of transfection as well as bacterial transformation. It has even been proposed that electroporation resulting from lightning strikes could be a mechanism of natural horizontal gene transfer.
This increase in permeability primarily affects transport of ions and other hydrated species, indicating that the mechanism is the creation of nm-scale water-filled holes in the membrane. Although electroporation and dielectric breakdown both result from application of an electric field, the mechanisms involved are fundamentally different. In dielectric breakdown the barrier material is ionized, creating a conductive pathway. The material alteration is thus chemical in nature. In contrast, during electroporation the lipid molecules are not chemically altered but simply shift position, opening up a pore that acts as the conductive pathway through the bilayer as it is filled with water.
Lipid bilayers are large enough structures to have some of the mechanical properties of liquids or solids. The area compression modulus Ka, bending modulus Kb, and edge energy , can be used to describe them. Solid lipid bilayers also have a shear modulus, but like any liquid, the shear modulus is zero for fluid bilayers. These mechanical properties affect how the membrane functions. Ka and Kb affect the ability of proteins and small molecules to insert into the bilayer, and bilayer mechanical properties have been shown to alter the function of mechanically activated ion channels. Bilayer mechanical properties also govern what types of stress a cell can withstand without tearing. Although lipid bilayers can easily bend, most cannot stretch more than a few percent before rupturing.
As discussed in the Structure and organization section, the hydrophobic attraction of lipid tails in water is the primary force holding lipid bilayers together. Thus, the elastic modulus of the bilayer is primarily determined by how much extra area is exposed to water when the lipid molecules are stretched apart. It is not surprising given this understanding of the forces involved that studies have shown that Ka varies strongly with osmotic pressure  but only weakly with tail length and unsaturation. Because the forces involved are so small, it is difficult to experimentally determine Ka. Most techniques require sophisticated microscopy and very sensitive measurement equipment.
In contrast to Ka, which is a measure of how much energy is needed to stretch the bilayer, Kb is a measure of how much energy is needed to bend or flex the bilayer. Formally, bending modulus is defined as the energy required to deform a membrane from its intrinsic curvature to some other curvature. Intrinsic curvature is defined by the ratio of the diameter of the head group to that of the tail group. For two-tailed PC lipids, this ratio is nearly one so the intrinsic curvature is nearly zero. If a particular lipid has too large a deviation from zero intrinsic curvature it will not form a bilayer and will instead form other phases such as micelles or inverted micelles. Typically, Kb is not measured experimentally but rather is calculated from measurements of Ka and bilayer thickness, since the three parameters are related.
is a measure of how much energy it takes to expose a bilayer edge to water by tearing the bilayer or creating a hole in it. The origin of this energy is the fact that creating such an interface exposes some of the lipid tails to water, but the exact orientation of these border lipids is unknown. There is some evidence that both hydrophobic (tails straight) and hydrophilic (heads curved around) pores can coexist.
Fusion is the process by which two lipid bilayers merge, resulting in one connected structure. If this fusion proceeds completely through both leaflets of both bilayers, a water-filled bridge is formed and the solutions contained by the bilayers can mix. Alternatively, if only one leaflet from each bilayer is involved in the fusion process, the bilayers are said to be hemifused. Fusion is involved in many cellular processes, in particular in eukaryotes, since the eukaryotic cell is extensively sub-divided by lipid bilayer membranes. Exocytosis, fertilization of an egg by sperm and transport of waste products to the lysozome are a few of the many eukaryotic processes that rely on some form of fusion. Even the entry of pathogens can be governed by fusion, as many bilayer-coated viruses have dedicated fusion proteins to gain entry into the host cell.
There are four fundamental steps in the fusion process. First, the involved membranes must aggregate, approaching each other to within several nanometers. Second, the two bilayers must come into very close contact (within a few angstroms). To achieve this close contact, the two surfaces must become at least partially dehydrated, as the bound surface water normally present causes bilayers to strongly repel. The presence of ions, in particular divalent cations like magnesium and calcium, strongly affects this step. One of the critical roles of calcium in the body is regulating membrane fusion. Third, a destabilization must form at one point between the two bilayers, locally distorting their structures. The exact nature of this distortion is not known. One theory is that a highly curved "stalk" must form between the two bilayers. Proponents of this theory believe that it explains why phosphatidylethanolamine, a highly curved lipid, promotes fusion. Finally, in the last step of fusion, this point defect grows and the components of the two bilayers mix and diffuse away from the site of contact.
The situation is further complicated when considering fusion in vivo since biological fusion is almost always regulated by the action of membrane-associated proteins. The first of these proteins to be studied were the viral fusion proteins, which allow an enveloped virus to insert its genetic material into the host cell (enveloped viruses are those surrounded by a lipid bilayer; some others have only a protein coat). Eukaryotic cells also use fusion proteins, the best-studied of which are the SNAREs. SNARE proteins are used to direct all vesicular intracellular trafficking. Despite years of study, much is still unknown about the function of this protein class. In fact, there is still an active debate regarding whether SNAREs are linked to early docking or participate later in the fusion process by facilitating hemifusion.
In studies of molecular and cellular biology it is often desirable to artificially induce fusion. The addition of polyethylene glycol (PEG) causes fusion without significant aggregation or biochemical disruption. This procedure is now used extensively, for example by fusing B-cells with melanoma cells. The resulting “hybridoma” from this combination expresses a desired antibody as determined by the B-cell involved, but is immortalized due to the melanoma component. Fusion can also be artificially induced through electroporation in a process known as electrofusion. It is believed that this phenomenon results from the energetically active edges formed during electroporation, which can act as the local defect point to nucleate stalk growth between two bilayers.
Lipid bilayers can be created artificially in the lab to allow researchers to perform experiments that cannot be done with natural bilayers. These synthetic systems are called model lipid bilayers. There are many different types of model bilayers, each having experimental advantages and disadvantages. They can be made with either synthetic or natural lipids. Among the most common model systems are:
- Black lipid membranes (BLM)
- Supported lipid bilayers (SLB)
- Tethered Bilayer Lipid Membranes (t-BLM)
To date, the most successful commercial application of lipid bilayers has been the use of liposomes for drug delivery, especially for cancer treatment. (Note- the term “liposome” is in essence synonymous with “vesicle” except that vesicle is a general term for the structure whereas liposome refers to only artificial not natural vesicles) The basic idea of liposomal drug delivery is that the drug is encapsulated in solution inside the liposome then injected into the patient. These drug-loaded liposomes travel through the system until they bind at the target site and rupture, releasing the drug. In theory, liposomes should make an ideal drug delivery system since they can isolate nearly any hydrophilic drug, can be grafted with molecules to target specific tissues and can be relatively non-toxic since the body possesses biochemical pathways for degrading lipids.
The first generation of drug delivery liposomes had a simple lipid composition and suffered from several limitations. Circulation in the bloodstream was extremely limited due to both renal clearing and phagocytosis. Refinement of the lipid composition to tune fluidity, surface charge density, and surface hydration resulted in vesicles that adsorb fewer proteins from serum and thus are less readily recognized by the immune system. The most significant advance in this area was the grafting of polyethylene glycol (PEG) onto the liposome surface to produce “stealth” vesicles, which circulate over long times without immune or renal clearing.
The first stealth liposomes were passively targeted at tumor tissues. Because tumors induce rapid and uncontrolled angiogenesis they are especially “leaky” and allow liposomes to exit the bloodstream at a much higher rate than normal tissue would. More recently[when?] work has been undertaken to graft antibodies or other molecular markers onto the liposome surface in the hope of actively binding them to a specific cell or tissue type. Some examples of this approach are already in clinical trials.
Another potential application of lipid bilayers is the field of biosensors. Since the lipid bilayer is the barrier between the interior and exterior of the cell, it is also the site of extensive signal transduction. Researchers over the years have tried to harness this potential to develop a bilayer-based device for clinical diagnosis or bioterrorism detection. Progress has been slow in this area and, although a few companies have developed automated lipid-based detection systems, they are still targeted at the research community. These include Biacore Life Sciences, which offers a disposable chip for utilizing lipid bilayers in studies of binding kinetics and Nanion Inc., which has developed an automated patch clamping system. Other, more exotic applications are also being pursued such as the use of lipid bilayer membrane pores for DNA sequencing by Oxford Nanolabs. To date, this technology has not proven commercially viable.
A supported lipid bilayer (SLB) as described above has achieved commercial success as a screening technique to measure the permeability of drugs. This parallel artificial membrane permeability assay PAMPA technique measures the permeability across specifically formulated lipid cocktail(s) found to be highly correlated with Caco-2 cultures, the gastrointestinal tract, blood–brain barrier and skin.
By the early twentieth century scientists had come to believe that cells are surrounded by a thin oil-like barrier, but the structural nature of this membrane was not known. Two experiments in 1925 laid the groundwork to fill in this gap. By measuring the capacitance of erythrocyte solutions, Hugo Fricke determined that the cell membrane was 3.3 nm thick.
Although the results of this experiment were accurate, Fricke misinterpreted the data to mean that the cell membrane is a single molecular layer. Prof. Dr. Evert Gorter (1881–1954) and F. Grendel of Leiden University approached the problem from a different perspective, spreading the erythrocyte lipids as a monolayer on a Langmuir-Blodgett trough. When they compared the area of the monolayer to the surface area of the cells, they found a ratio of two to one. Later analyses showed several errors and incorrect assumptions with this experiment but, serendipitously, these errors canceled out and from this flawed data Gorter and Grendel drew the correct conclusion- that the cell membrane is a lipid bilayer.
This theory was confirmed through the use of electron microscopy in the late 1950s. Although he did not publish the first electron microscopy study of lipid bilayers J. David Robertson was the first to assert that the two dark electron-dense bands were the headgroups and associated proteins of two apposed lipid monolayers. In this body of work, Robertson put forward the concept of the “unit membrane.” This was the first time the bilayer structure had been universally assigned to all cell membranes as well as organelle membranes.
Around the same time, the development of model membranes confirmed that the lipid bilayer is a stable structure that can exist independent of proteins. By “painting” a solution of lipid in organic solvent across an aperture, Mueller and Rudin were able to create an artificial bilayer and determine that this exhibited lateral fluidity, high electrical resistance and self-healing in response to puncture, all of which properties of a natural cell membrane. A few years later, Alec Bangham showed that bilayers, in the form of lipid vesicles, could also be formed simply by exposing a dried lipid sample to water. This was an important advance, since it demonstrated that lipid bilayers form spontaneously via self assembly and do not require a patterned support structure.
- Mashaghi et al. Hydration strongly affects the molecular and electronic structure of membrane phospholipids. 136, 114709 (2012)
- Lewis BA, Engelman DM (May 1983). "Lipid bilayer thickness varies linearly with acyl chain length in fluid phosphatidylcholine vesicles". J. Mol. Biol. 166 (2): 211–7. doi:10.1016/S0022-2836(83)80007-2. PMID 6854644.
- Zaccai G, Blasie JK, Schoenborn BP (January 1975). "Neutron Diffraction Studies on the Location of Water in Lecithin Bilayer Model Membranes". Proc. Natl. Acad. Sci. U.S.A. 72 (1): 376–380. Bibcode:1975PNAS...72..376Z. doi:10.1073/pnas.72.1.376. PMC 432308. PMID 16592215.
- Nagle JF, Tristram-Nagle S (November 2000). "Structure of lipid bilayers". Biochim. Biophys. Acta 1469 (3): 159–95. doi:10.1016/S0304-4157(00)00016-2. PMC 2747654. PMID 11063882.
- Parker J, Madigan MT, Brock TD, Martinko JM (2003). Brock biology of microorganisms (10th ed.). Englewood Cliffs, N.J: Prentice Hall. ISBN 0-13-049147-0.
- Marsh D (July 2001). "Polarity and permeation profiles in lipid membranes". Proc. Natl. Acad. Sci. U.S.A. 98 (14): 7777–82. Bibcode:2001PNAS...98.7777M. doi:10.1073/pnas.131023798. PMC 35418. PMID 11438731.
- Marsh D (December 2002). "Membrane water-penetration profiles from spin labels". Eur. Biophys. J. 31 (7): 559–62. doi:10.1007/s00249-002-0245-z. PMID 12602343.
- Rawicz W, Olbrich KC, McIntosh T, Needham D, Evans E (July 2000). "Effect of chain length and unsaturation on elasticity of lipid bilayers". Biophys. J. 79 (1): 328–39. Bibcode:2000BpJ....79..328R. doi:10.1016/S0006-3495(00)76295-3. PMC 1300937. PMID 10866959.
- Trauble H, Haynes DH (1971). "The volume change in lipid bilayer lamellae at the crystalline-liquid crystalline phase transition". Chem. Phys. Lipids. 7 (4): 324–35. doi:10.1016/0009-3084(71)90010-7.
- Bretscher MS, (March 1972). "Asymmetrical lipid bilayer structure for biological membranes". Nature 236 (61): 11–12. Bibcode:1972Natur.236...11O. doi:10.1038/236011a0. PMID 4502419.
- Verkleij AJ, Zwaal RF, Roelofsen B, Comfurius P, Kastelijn D, van Deenen LL (October 1973). "The asymmetric distribution of phospholipids in the human red cell membrane. A combined study using phospholipases and freeze-etch electron microscopy". Biochim. Biophys. Acta 323 (2): 178–93. doi:10.1016/0005-2736(73)90143-0. PMID 4356540.
- Bell RM, Ballas LM, Coleman RA (1 March 1981). "Lipid topogenesis". J. Lipid Res. 22 (3): 391–403. PMID 7017050.
- Bretscher MS (August 1973). "Membrane structure: some general principles". Science 181 (4100): 622–629. Bibcode:1973Sci...181..622B. doi:10.1126/science.181.4100.622. PMID 4724478.
- Rothman JE, Kennedy EP (May 1977). "Rapid transmembrane movement of newly synthesized phospholipids during membrane assembly". Proc. Natl. Acad. Sci. U.S.A. 74 (5): 1821–5. Bibcode:1977PNAS...74.1821R. doi:10.1073/pnas.74.5.1821. PMC 431015. PMID 405668.
- Kornberg RD, McConnell HM (March 1971). "Inside-outside transitions of phospholipids in vesicle membranes". Biochemistry 10 (7): 1111–20. doi:10.1021/bi00783a003. PMID 4324203.
- Litman BJ (July 1974). "Determination of molecular asymmetry in the phosphatidylethanolamine surface distribution in mixed phospholipid vesicles". Biochemistry 13 (14): 2844–8. doi:10.1021/bi00711a010. PMID 4407872.
- Crane JM, Kiessling V, Tamm LK (February 2005). "Measuring lipid asymmetry in planar supported bilayers by fluorescence interference contrast microscopy". Langmuir 21 (4): 1377–88. doi:10.1021/la047654w. PMID 15697284.
- Kalb E, Frey S, Tamm LK (January 1992). "Formation of supported planar bilayers by fusion of vesicles to supported phospholipid monolayers". Biochim. Biophys. Acta 1103 (2): 307–16. doi:10.1016/0005-2736(92)90101-Q. PMID 1311950.
- Lin WC, Blanchette CD, Ratto TV, Longo ML (January 2006). "Lipid asymmetry in DLPC/DSPC-supported lipid bilayers: a combined AFM and fluorescence microscopy study". Biophys. J. 90 (1): 228–37. Bibcode:2006BpJ....90..228L. doi:10.1529/biophysj.105.067066. PMC 1367021. PMID 16214871.
- Berg, Howard C. (1993). Random walks in biology (Extended Paperback ed.). Princeton, N.J: Princeton University Press. ISBN 0-691-00064-6.
- Dietrich C, Volovyk ZN, Levi M, Thompson NL, Jacobson K (September 2001). "Partitioning of Thy-1, GM1, and cross-linked phospholipid analogs into lipid rafts reconstituted in supported model membrane monolayers". Proc. Natl. Acad. Sci. U.S.A. 98 (19): 10642–7. Bibcode:2001PNAS...9810642D. doi:10.1073/pnas.191168698. PMC 58519. PMID 11535814.
- Yeagle, Philip (1993). The membranes of cells (2nd ed.). Boston: Academic Press. ISBN 0-12-769041-7.
- Fadok VA, Bratton DL, Frasch SC, Warner ML, Henson PM (July 1998). "The role of phosphatidylserine in recognition of apoptotic cells by phagocytes". Cell Death Differ. 5 (7): 551–62. doi:10.1038/sj.cdd.4400404. PMID 10200509.
- Anderson HC, Garimella R, Tague SE (January 2005). "The role of matrix vesicles in growth plate development and biomineralization". Front. Biosci. 10 (1–3): 822–37. doi:10.2741/1576. PMID 15569622.
- Eanes ED, Hailer AW (January 1987). "Calcium phosphate precipitation in aqueous suspensions of phosphatidylserine-containing anionic liposomes". Calcif. Tissue Int. 40 (1): 43–8. doi:10.1007/BF02555727. PMID 3103899.
- Kim J, Mosior M, Chung LA, Wu H, McLaughlin S (July 1991). "Binding of peptides with basic residues to membranes containing acidic phospholipids". Biophys. J. 60 (1): 135–48. Bibcode:1991BpJ....60..135K. doi:10.1016/S0006-3495(91)82037-9. PMC 1260045. PMID 1883932.
- Koch AL (1984). "Primeval cells: possible energy-generating and cell-division mechanisms". J. Mol. Evol. 21 (3): 270–7. doi:10.1007/BF02102359. PMID 6242168.
- Alberts, Bruce (2002). Molecular biology of the cell (4th ed.). New York: Garland Science. ISBN 0-8153-4072-9.
- Martelli PL, Fariselli P, Casadio R (2003). "An ENSEMBLE machine learning approach for the prediction of all-alpha membrane proteins". Bioinformatics 19 (Suppl 1): i205–11. doi:10.1093/bioinformatics/btg1027. PMID 12855459.
- Filmore D (2004). "It's A GPCR World". Modern Drug Discovery 11: 24–9.
- Melikov KC, Frolov VA, Shcherbakov A, Samsonov AV, Chizmadzhev YA, Chernomordik LV (April 2001). "Voltage-induced nonconductive pre-pores and metastable single pores in unmodified planar lipid bilayer". Biophys. J. 80 (4): 1829–36. Bibcode:2001BpJ....80.1829M. doi:10.1016/S0006-3495(01)76153-X. PMC 1301372. PMID 11259296.
- Neher E, Sakmann B (April 1976). "Single-channel currents recorded from membrane of denervated frog muscle fibres". Nature 260 (5554): 799–802. Bibcode:1976Natur.260..799N. doi:10.1038/260799a0. PMID 1083489.
- Y. Roiter, M. Ornatska, A. R. Rammohan, J. Balakrishnan, D. R. Heine, and S. Minko, Interaction of Nanoparticles with Lipid Membrane, Nano Letters, vol. 8, iss. 3, pp. 941–944 (2008).
- Heuser JE, Reese TS, Dennis MJ, Jan Y, Jan L, Evans L (May 1979). "Synaptic vesicle exocytosis captured by quick freezing and correlated with quantal transmitter release". J. Cell Biol. 81 (2): 275–300. doi:10.1083/jcb.81.2.275. PMC 2110310. PMID 38256.
- Dubinnyi MA, Lesovoy DM, Dubovskii PV, Chupin VV, Arseniev AS (Jun 2006). "Modeling of 31P-NMR spectra of magnetically oriented phospholipid liposomes: A new analytical solution". Solid State Nucl Magn Reson. 29 (4): 305–311. doi:10.1016/j.ssnmr.2005.10.009. PMID 16298110.
- Tokumasu F, Jin AJ, Dvorak JA (2002). "Lipid membrane phase behavior elucidated in real time by controlled environment atomic force microscopy". J. Electron Micros. 51 (1): 1–9. doi:10.1093/jmicro/51.1.1. PMID 12003236.
- Richter RP, Brisson A (2003). "Characterization of lipid bilayers and protein assemblies supported on rough surfaces by atomic force microscopy". Langmuir 19 (5): 1632–40. doi:10.1021/la026427w.
- Steltenkamp S, Müller MM, Deserno M, Hennesthal C, Steinem C, Janshoff A (July 2006). "Mechanical properties of pore-spanning lipid bilayers probed by atomic force microscopy". Biophys. J. 91 (1): 217–26. Bibcode:2006BpJ....91..217S. doi:10.1529/biophysj.106.081398. PMC 1479081. PMID 16617084.
- Alireza Mashaghi et al., Hydration strongly affects the molecular and electronic structure of membrane phospholipids. J. Chem. Phys. 136, 114709 (2012) http://jcp.aip.org/resource/1/jcpsa6/v136/i11/p114709_s1
- M. Bonn et al., Structural inhomogeneity of interfacial water at lipid monolayers revealed by surface-specific vibrational pump-probe spectroscopy, J. Am. Chem. Soc. 132, 14971–14978 (2010).
- Mischa Bonn et al., Interfacial Water Facilitates Energy Transfer by Inducing Extended Vibrations in Membrane Lipids, J Phys Chem, 2012 http://pubs.acs.org/doi/abs/10.1021/jp302478a
- Chakrabarti AC (1994). "Permeability of membranes to amino acids and modified amino acids: mechanisms involved in translocation". Amino Acids 6 (3): 213–29. doi:10.1007/BF00813743. PMID 11543596.
- Hauser H, Phillips MC, Stubbs M (October 1972). "Ion permeability of phospholipid bilayers". Nature 239 (5371): 342–4. Bibcode:1972Natur.239..342H. doi:10.1038/239342a0. PMID 12635233.
- Papahadjopoulos D, Watkins JC (September 1967). "Phospholipid model membranes. II. Permeability properties of hydrated liquid crystals". Biochim. Biophys. Acta 135 (4): 639–52. doi:10.1016/0005-2736(67)90095-8. PMID 6048247.
- Paula S, Volkov AG, Van Hoek AN, Haines TH, Deamer DW (January 1996). "Permeation of protons, potassium ions, and small polar molecules through phospholipid bilayers as a function of membrane thickness". Biophys. J. 70 (1): 339–48. Bibcode:1996BpJ....70..339P. doi:10.1016/S0006-3495(96)79575-9. PMC 1224932. PMID 8770210.
- Xiang TX, Anderson BD (June 1994). "The relationship between permeant size and permeability in lipid bilayer membranes". J. Membr. Biol. 140 (2): 111–22. PMID 7932645.
- Gouaux E, Mackinnon R (December 2005). "Principles of selective ion transport in channels and pumps". Science 310 (5753): 1461–5. Bibcode:2005Sci...310.1461G. doi:10.1126/science.1113666. PMID 16322449.
- Gundelfinger ED, Kessels MM, Qualmann B (February 2003). "Temporal and spatial coordination of exocytosis and endocytosis". Nat. Rev. Mol. Cell Biol. 4 (2): 127–39. doi:10.1038/nrm1016. PMID 12563290.
- Steinman RM, Brodie SE, Cohn ZA (March 1976). "Membrane flow during pinocytosis. A stereologic analysis". J. Cell Biol. 68 (3): 665–87. doi:10.1083/jcb.68.3.665. PMC 2109655. PMID 1030706.
- Neumann E, Schaefer-Ridder M, Wang Y, Hofschneider PH (1982). "Gene transfer into mouse lyoma cells by electroporation in high electric fields". EMBO J. 1 (7): 841–5. PMC 553119. PMID 6329708.
- Demanèche S, Bertolla F, Buret F, et al. (August 2001). "Laboratory-scale evidence for lightning-mediated gene transfer in soil". Appl. Environ. Microbiol. 67 (8): 3440–4. doi:10.1128/AEM.67.8.3440-3444.2001. PMC 93040. PMID 11472916.
- Garcia ML (July 2004). "Ion channels: gate expectations". Nature 430 (6996): 153–5. Bibcode:2004Natur.430..153G. doi:10.1038/430153a. PMID 15241399.
- McIntosh TJ, Simon SA (2006). "Roles of Bilayer Material Properties in Function and Distribution of Membrane Proteins". Annu. Rev. Biophys. Biomol. Struct. 35 (1): 177–98. doi:10.1146/annurev.biophys.35.040405.102022. PMID 16689633.
- Suchyna TM, Tape SE, Koeppe RE, Andersen OS, Sachs F, Gottlieb PA (July 2004). "Bilayer-dependent inhibition of mechanosensitive channels by neuroactive peptide enantiomers". Nature 430 (6996): 235–40. Bibcode:2004Natur.430..235S. doi:10.1038/nature02743. PMID 15241420.
- Hallett FR, Marsh J, Nickel BG, Wood JM (February 1993). "Mechanical properties of vesicles. II. A model for osmotic swelling and lysis". Biophys. J. 64 (2): 435–42. Bibcode:1993BpJ....64..435H. doi:10.1016/S0006-3495(93)81384-5. PMC 1262346. PMID 8457669.
- Boal, David H. (2001). Mechanics of the cell. Cambridge, UK: Cambridge University Press. ISBN 0-521-79681-4.
- Rutkowski CA, Williams LM, Haines TH, Cummins HZ (June 1991). "The elasticity of synthetic phospholipid vesicles obtained by photon correlation spectroscopy". Biochemistry 30 (23): 5688–96. doi:10.1021/bi00237a008. PMID 2043611.
- Evans E, Heinrich V, Ludwig F, Rawicz W (October 2003). "Dynamic tension spectroscopy and strength of biomembranes". Biophys. J. 85 (4): 2342–50. Bibcode:2003BpJ....85.2342E. doi:10.1016/S0006-3495(03)74658-X. PMC 1303459. PMID 14507698.
- Weaver JC, Chizmadzhev YA (1996). "Theory of electroporation: A review". Biochemistry and Bioenergetics 41 (2): 135–60. doi:10.1016/S0302-4598(96)05062-3.
- Papahadjopoulos D, Nir S, Düzgünes N (April 1990). "Molecular mechanisms of calcium-induced membrane fusion". J. Bioenerg. Biomembr. 22 (2): 157–79. doi:10.1007/BF00762944. PMID 2139437.
- Leventis R, Gagné J, Fuller N, Rand RP, Silvius JR (November 1986). "Divalent cation induced fusion and lipid lateral segregation in phosphatidylcholine-phosphatidic acid vesicles". Biochemistry 25 (22): 6978–87. doi:10.1021/bi00370a600. PMID 3801406.
- Markin VS, Kozlov MM, Borovjagin VL (October 1984). "On the theory of membrane fusion. The stalk mechanism". Gen. Physiol. Biophys. 3 (5): 361–77. PMID 6510702.
- Chernomordik LV, Kozlov MM (2003). "Protein-lipid interplay in fusion and fission of biological membranes". Annu. Rev. Biochem. 72 (1): 175–207. doi:10.1146/annurev.biochem.72.121801.161504. PMID 14527322.
- Georgiev, Danko D .; James F . Glazebrook (2007). "Subneuronal processing of information by solitary waves and stochastic processes". In Lyshevski, Sergey Edward. Nano and Molecular Electronics Handbook. Nano and Microengineering Series. CRC Press. pp. 17–1–17–41. ISBN 978-0-8493-8528-5.
- Chen YA, Scheller RH (February 2001). "SNARE-mediated membrane fusion". Nat. Rev. Mol. Cell Biol. 2 (2): 98–106. doi:10.1038/35052017. PMID 11252968.
- Köhler G, Milstein C (August 1975). "Continuous cultures of fused cells secreting antibody of predefined specificity". Nature 256 (5517): 495–7. Bibcode:1975Natur.256..495K. doi:10.1038/256495a0. PMID 1172191.
- Jordan, Carol A.; Neumann, Eberhard; Sowershi mason, Arthur E. (1989). Electroporation and electrofusion in cell biology. New York: Plenum Press. ISBN 0-306-43043-6.
- Immordino ML, Dosio F, Cattel L (2006). "Stealth liposomes: review of the basic science, rationale, and clinical applications, existing and potential". Int J Nanomedicine 1 (3): 297–315. doi:10.2217/174358184.108.40.2067. PMC 2426795. PMID 17717971.
- Chonn A, Semple SC, Cullis PR (15 September 1992). "Association of blood proteins with large unilamellar liposomes in vivo. Relation to circulation lifetimes". J. Biol. Chem. 267 (26): 18759–65. PMID 1527006.
- Boris EH, Winterhalter M, Frederik PM, Vallner JJ, Lasic DD (1997). "Stealth liposomes: from theory to product". Advanced Drug Delivery Reviews 24 (2–3): 165–77. doi:10.1016/S0169-409X(96)00456-5.
- Maeda H, Sawa T, Konno T (July 2001). "Mechanism of tumor-targeted delivery of macromolecular drugs, including the EPR effect in solid tumor and clinical overview of the prototype polymeric drug SMANCS". J Control Release 74 (1–3): 47–61. doi:10.1016/S0168-3659(01)00309-1. PMID 11489482.
- Lopes DE, Menezes DE, Kirchmeier MJ, Gagne JF (1999). "Cellular trafficking and cytotoxicity of anti-CD19-targeted liposomal doxorubicin in B lymphoma cells". Journal of Liposome Research 9 (2): 199–228. doi:10.3109/08982109909024786.
- Matsumura Y, Gotoh M, Muro K, et al. (March 2004). "Phase I and pharmacokinetic study of MCC-465, a doxorubicin (DXR) encapsulated in PEG immunoliposome, in patients with metastatic stomach cancer". Ann. Oncol. 15 (3): 517–25. doi:10.1093/annonc/mdh092. PMID 14998859.
- Biacore A100 System Information. Biacore Inc. Retrieved Feb 12, 2009.
- Nanion Technologies. Automated Patch Clamp. Retrieved Feb 28, 2010. (PDF)
- Bermejo, M. et al. (2004). PAMPA – a drug absorption in vitro model 7. Comparing rat in situ, Caco-2, and PAMPA permeability of fluoroquinolones. Pharm. Sci., 21: 429-441.
- Avdeef, A. et al. (2005). Caco-2 permeability of weakly basic drugs predicted with the Double-Sink PAMPA pKaflux method. Pharm. Sci., 24: 333-349.
- Avdeef, A. et al. (2004). PAMPA – a drug absorption in vitro model 11. Matching the in vivo unstirred water layer thickness by individual-well stirring in microtitre plates. Pharm. Sci., 22: 365-374.
- Dagenais, C. et al. (2009). P-glycoprotein deficient mouse in situ blood–brain barrier permeability and its prediction using an in combo PAMPA model. Eur. J. Phar. Sci., 38(2): 121-137.
- Sinkó, B. et al. (2009). A PAMPA Study of the Permeability-Enhancing Effect of New Ceramide Analogues. Chemistry & Biodiversity, 6: 1867-1874.
- Loeb J (December 1904). "The recent development of Biology". Science 20 (519): 777–786. Bibcode:1904Sci....20..777L. doi:10.1126/science.20.519.777. PMID 17730464.
- Fricke H (1925). "The electrical capacity of suspensions with special reference to blood". Journal of General Physiology 9 (2): 137–52. doi:10.1085/jgp.9.2.137. PMC 2140799. PMID 19872238.
- Dooren L J, Wiedemann L R (1986). "On bimolecular layers of lipids on the chromocytes of the blood". Journal of European Journal of Pediatrics 145 (5): 329. doi:10.1007/BF00439232.
- Gorter E, Grendel F (1925). "On bimolecular layers of lipids on the chromocytes of the blood". Journal of Experimental Medicine 41 (4): 439–43. doi:10.1084/jem.41.4.439. PMC 2130960. PMID 19868999.
- Sjöstrand FS, Andersson-Cedergren E, Dewey MM (April 1958). "The ultrastructure of the intercalated discs of frog, mouse and guinea pig cardiac muscle". J. Ultrastruct. Res. 1 (3): 271–87. doi:10.1016/S0022-5320(58)80008-8. PMID 13550367.
- Robertson JD (1960). "The molecular structure and contact relationships of cell membranes". Prog. Biophys. Mol. Biol. 10: 343–418. PMID 13742209.
- Robertson JD (1959). "The ultrastructure of cell membranes and their derivatives". Biochem. Soc. Symp. 16: 3–43. PMID 13651159.
- Mueller P, Rudin DO, Tien HT, Wescott WC (June 1962). "Reconstitution of cell membrane structure in vitro and its transformation into an excitable system". Nature 194 (4832): 979–80. Bibcode:1962Natur.194..979M. doi:10.1038/194979a0. PMID 14476933.
- Bangham, A. D.; Horne, R. W. (1964). "Negative Staining of Phospholipids and Their Structural Modification by Surface-Active Agents As Observed in the Electron Microscope". Journal of Molecular Biology 8: 660–668. doi:10.1016/S0022-2836(64)80115-7. PMID 14187392.
- Avanti Lipids One of the largest commercial suppliers of lipids. Technical information on lipid properties and handling and lipid bilayer preparation techniques.
- LIPIDAT An extensive database of lipid physical properties
- Structure of Fluid Lipid Bilayers Simulations and publication links related to the cross sectional structure of lipid bilayers.
- Lipid Bilayers and the Gramicidin Channel (requires Java plugin) Pictures and movies showing the results of molecular dynamics simulations of lipid bilayers.
- Structure of Fluid Lipid Bilayers, from the Stephen White laboratory at University of California, Irvine
- Animations of lipid bilayer dynamics (requires Flash plugin)