Liquid chromatography–mass spectrometry

Liquid chromatography–mass spectrometry

Acronym	LCMS
Classification	Chromatography Mass spectrometry
Analytes	organic molecules biomolecules
Manufacturers	Agilent Bruker MDS PerkinElmer Shimadzu Scientific Thermo Fisher Scientific Varian, Inc. Waters Corporation
Other techniques
Related	Gas chromatography–mass spectrometry

Liquid chromatography–mass spectrometry (LC-MS, or alternatively HPLC-MS) is a chemistry technique that combines the physical separation capabilities of liquid chromatography (or HPLC) with the mass analysis capabilities of mass spectrometry. LC-MS is a powerful technique used for many applications which has very high sensitivity and selectivity. Generally its application is oriented towards the general detection and potential identification of chemicals in the presence of other chemicals (in a complex mixture). Preparative LC-MS system can be used for fast and mass directed purification of natural-products extracts and new molecular entities important to food, pharmaceutical, agrochemical and other industries. The limitations of LC-MS in urine analysis drug screening is that it often fails to distinguish between specific metabolites, in particular with hydrocodone and its metabolites. LC-MS urine analysis testing is used to detect specific categories of drugs. However, gas chromatography (GC-MS) should be used when detection of a specific drug and its metabolites is required.

Liquid chromatography

Flow splitting

When standard bore (4.6 mm) columns are used the flow is often split ~10:1. This can be beneficial by allowing the use of other techniques in tandem such as MS and UV. However splitting the flow to UV will decrease the sensitivity of spectrophotometric detectors. The mass spectrometry on the other hand will give improved sensitivity at flow rates of 200 μL/min or less.

Mass spectrometry

Mass spectrometry (MS) is an analytical technique that measures the mass-to-charge ratio of charged particles.[1] It is used for determining masses of particles, for determining the elemental composition of a sample or molecule, and for elucidating the chemical structures of molecules, such as peptides and other chemical compounds. MS works by ionizing chemical compounds to generate charged molecules or molecule fragments and measuring their mass-to-charge ratios.[1] In a typical MS procedure:

A sample is loaded onto the MS instrument and undergoes vaporization.

The components of the sample are ionized by one of a variety of methods (e.g., by impacting them with an electron beam), which results in the formation of charged particles (ions).

The ions are separated according to their mass-to-charge ratio in an analyzer by electromagnetic fields

The ions are detected, usually by a quantitative method.

The ion signal is processed into mass spectra.

Additionally, MS instruments consist of three modules:

An ion source, which can convert gas phase sample molecules into ions (or, in the case of electrospray ionization, move ions that exist in solution into the gas phase)

A mass analyzer, which sorts the ions by their masses by applying electromagnetic fields

A detector, which measures the value of an indicator quantity and thus provides data for calculating the abundances of each ion present

The technique has both qualitative and quantitative uses. These include identifying unknown compounds, determining the isotopic composition of elements in a molecule, and determining the structure of a compound by observing its fragmentation. Other uses include quantifying the amount of a compound in a sample or studying the fundamentals of gas phase ion chemistry (the chemistry of ions and neutrals in a vacuum). MS is now in very common use in analytical laboratories that study physical, chemical, or biological properties of a great variety of compounds.

Mass analyzer

There are many different mass analyzers that can be used in LC/MS. Single quadrupole, triple quadrupole, ion trap, time of flight (TOF) and quadrupole-time of flight (Q-TOF).

Interface

Understandably the interface between a liquid phase technique which continuously flows liquid, and a gas phase technique carried out in a vacuum was difficult for a long time. The advent of electrospray ionization changed this. The interface is most often an electrospray ion source or variant such as a nanospray source; however atmospheric pressure chemical ionization interface is also used. Various deposition and drying techniques have also been used such as using moving belts; however the most common of these is off-line MALDI deposition. A new approach still under development called Direct-EI LC-MS interface, couples a nano HPLC system and an electron ionization equipped mass spectrometer.

Applications

Pharmacokinetics

LC-MS is very commonly used in pharmacokinetic studies of pharmaceuticals and is thus the most frequently used technique in the field of bioanalysis. These studies give information about how quickly a drug will be cleared from the hepatic blood flow, and organs of the body. MS is used for this due to high sensitivity and exceptional specificity compared to UV (as long as the analyte can be suitably ionised), and short analysis time.

The major advantage MS has is the use of tandem MS-MS. The detector may be programmed to select certain ions to fragment. The process is essentially a selection technique, but is in fact more complex. The measured quantity is the sum of molecule fragments chosen by the operator. As long as there are no interferences or ion suppression, the LC separation can be quite quick. It is common now to have analysis times of 1 minute or less by MS-MS detection, compared to over 10 mins with UV detection.

Proteomics/metabolomics

LC-MS is also used in proteomics where again components of a complex mixture must be detected and identified in some manner. The bottom-up proteomics LC-MS approach to proteomics generally involves protease digestion and denaturation (usually trypsin as a protease, urea to denature tertiary structure and iodoacetamide to cap cysteine residues) followed by LC-MS with peptide mass fingerprinting or LC-MS/MS (tandem MS) to derive sequence of individual peptides. LC-MS/MS is most commonly used for proteomic analysis of complex samples where peptide masses may overlap even with a high-resolution mass spectrometer. Samples of complex biological fluids like human serum may be run in a modern LC-MS/MS system and result in over 1000 proteins being identified, provided that the sample was first separated on an SDS-PAGE gel or HPLC-SCX.^{[citation needed]}

Profiling of secondary metabolites in plants or food like phenolics can be achieved with liquid chromatography–mass spectrometry.

Drug development

LC-MS is frequently used in drug development at many different stages including peptide mapping, glycoprotein mapping, natural products dereplication, bioaffinity screening, in vivo drug screening, metabolic stability screening, metabolite identification, impurity identification, quantitative bioanalysis, and quality control.

See also

• Gas chromatography–mass spectrometry
• Capillary electrophoresis–mass spectrometry
• Ion-mobility spectrometry–mass spectrometry

Bibliography

• Thurman, E. M.; Ferrer, Imma (2003). Liquid chromatography/mass spectrometry, MS/MS and time of flight MS: analysis of emerging contaminants. Columbus, OH: American Chemical Society. ISBN 0-8412-3825-1. 
• Ferrer, Imma; Thurman, E. M. (2009). Liquid chromatography-Time of Flight Mass Spectrometry: Principles, Tools and Applications for Accurate Mass Analysis. New York, NJ: Wiley. ISBN 978-0-470-13797-0. 
• Ardrey, R. E.; Ardrey, Robert (2003). Liquid chromatography-mass spectrometry: an introduction. London: J. Wiley. ISBN 0-471-49801-7. 
• McMaster, Marvin C. (2005). LC/MS: a practical user's guide. New York: John Wiley. ISBN 0-471-65531-7. 
• Wilfried M.A. Niessen, Wilfried M. Niessen (2006). Liquid Chromatography-Mass Spectrometry, Third Edition (Chromatographic Science). Boca Raton: CRC. ISBN 0-8247-4082-3. 
• Yergey, Alfred L. (1990). Liquid chromatography/mass spectrometry: techniques and applications. New York: Plenum Press. ISBN 0-306-43186-6.