Lucifer yellow

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Lucifer yellow
Lucifer yellow.png
PubChem 20835957
ChemSpider 20137740 N
ChEBI CHEBI:52104 YesY
Jmol-3D images Image 1
Molecular formula C13H10Li2N4O9S2
Molar mass 444.25 g mol−1
Except where noted otherwise, data are given for materials in their standard state (at 25 °C (77 °F), 100 kPa)
 N (verify) (what is: YesY/N?)
Infobox references

Lucifer yellow is a fluorescent dye used in cell biology.[1] The key property of Lucifer yellow is that it can readily visualized in both living and fixed cells using a fluorescence microscope. Lucifer yellow was engineered by Walter W. Stewart at NIH and patented in 1978.[2]


For common usage it is compounded with carbohydrazide (CH) and prepared as a Lithium salt. The CH group allows it to be covalently linked to surrounding biomolecules during aldehyde fixation.[3]

Other cations such as ammonium or potassium can be used when lithium is undesirable, but the resulting salts are less soluble in water.

Lucifer yellow can also be compounded as a vinyl sulfone, with ethylenediamine, or with cadaverine.


  1. ^ Hanani, Menachem (January 2012). "Lucifer yellow – an angel rather than the devil". Journal of Cellular and Molecular Medicine 16 (2): 22–31. doi:10.1111/j.1582-4934.2011.01378.x. PMID 21740513. 
  2. ^ Patent description
  3. ^ "Lucifer Yellow CH, Lithium Salt". Molecular Probes®. Retrieved 17 March 2014.