Lumazine synthase

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6,7-dimethyl-8-ribityllumazine synthase
EC number
IntEnz IntEnz view
ExPASy NiceZyme view
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum

Lumazine synthase (EC, 6,7-dimethyl-8-ribityllumazine synthase, 6,7-dimethyl-8-ribityllumazine synthase 2, 6,7-dimethyl-8-ribityllumazine synthase 1, lumazine synthase 2, lumazine synthase 1, type I lumazine synthase, type II lumazine synthase, RIB4, MJ0303, RibH, Pbls, MbtLS, RibH1 protein, RibH2 protein, RibH1, RibH2) is an enzyme with system name 5-amino-6-(D-ribitylamino)uracil butanedionetransferase.[1][2][3][4][5][6][7][8][9][10][11][12][13][14] This enzyme catalyses the following chemical reaction

1-deoxy-L-glycero-tetrulose 4-phosphate + 5-amino-6-(D-ribitylamino)uracil \rightleftharpoons 6,7-dimethyl-8-(D-ribityl)lumazine + 2 H2O + phosphate

This enzyme is involved in riboflavin biosynthesis.


  1. ^ Kis, K., Volk, R. and Bacher, A. (1995). "Biosynthesis of riboflavin. Studies on the reaction mechanism of 6,7-dimethyl-8-ribityllumazine synthase". Biochemistry 34 (9): 2883–2892. doi:10.1021/bi00009a019. PMID 7893702. 
  2. ^ Garcia-Ramirez, J.J., Santos, M.A. and Revuelta, J.L. (1995). "The Saccharomyces cerevisiae RIB4 gene codes for 6,7-dimethyl-8-ribityllumazine synthase involved in riboflavin biosynthesis. Molecular characterization of the gene and purification of the encoded protein". J. Biol. Chem. 270 (40): 23801–23807. doi:10.1074/jbc.270.40.23801. PMID 7559556. 
  3. ^ Bacher, A., Fischer, M., Kis, K., Kugelbrey, K., Mörtl, S., Scheuring, J., Weinkauf, S., Eberhardt, S., Schmidt-Bäse, K., Huber, R., Ritsert, K., Cushman, M., Ladenstein, R. (1996). "Biosynthesis of riboflavin: structure and mechanism of lumazine synthase". Biochem. Soc. Trans. 24 (1): 89–94. PMID 8674771. 
  4. ^ Mörtl, S., Fischer, M., Richter, G., Tack, J., Weinkauf, S. and Bacher, A. (1996). "Biosynthesis of riboflavin. Lumazine synthase of Escherichia coli". J. Biol. Chem. 271 (52): 33201–33207. doi:10.1074/jbc.271.52.33201. PMID 8969176. 
  5. ^ Bacher, A., Eberhardt, S., Fischer, M., Mortl, S., Kis, K., Kugelbrey, K., Scheuring, J. and Schott, K. (1997). "Biosynthesis of riboflavin: lumazine synthase and riboflavin synthase". Methods Enzymol. Methods in Enzymology 280: 389–399. doi:10.1016/S0076-6879(97)80130-9. ISBN 9780121821814. PMID 9211334. 
  6. ^ Goldbaum, F.A., Velikovsky, C.A., Baldi, P.C., Mortl, S., Bacher, A. and Fossati, C.A. (1999). "The 18-kDa cytoplasmic protein of Brucella species - an antigen useful for diagnosis - is a lumazine synthase". J. Med. Microbiol. 48 (9): 833–839. doi:10.1099/00222615-48-9-833. PMID 10482294. 
  7. ^ Jordan, D.B., Bacot, K.O., Carlson, T.J., Kessel, M. and Viitanen, P.V. (1999). "Plant riboflavin biosynthesis. Cloning, chloroplast localization, expression, purification, and partial characterization of spinach lumazine synthase". J. Biol. Chem. 274 (31): 22114–22121. doi:10.1074/jbc.274.31.22114. PMID 10419541. 
  8. ^ Zhang, X., Meining, W., Fischer, M., Bacher, A. and Ladenstein, R. (2001). "X-ray structure analysis and crystallographic refinement of lumazine synthase from the hyperthermophile Aquifex aeolicus at 1.6 Å resolution: determinants of thermostability revealed from structural comparisons". J. Mol. Biol. 306 (5): 1099–1114. doi:10.1006/jmbi.2000.4435. PMID 11237620. 
  9. ^ Fischer, M., Haase, I., Feicht, R., Richter, G., Gerhardt, S., Changeux, J.P., Huber, R. and Bacher, A. (2002). "Biosynthesis of riboflavin: 6,7-dimethyl-8-ribityllumazine synthase of Schizosaccharomyces pombe". Eur. J. Biochem. 269 (2): 519–526. doi:10.1046/j.0014-2956.2001.02674.x. PMID 11856310. 
  10. ^ Cushman, M., Yang, D., Gerhardt, S., Huber, R., Fischer, M., Kis, K. and Bacher, A. (2002). "Design, synthesis, and evaluation of 6-carboxyalkyl and 6-phosphonoxyalkyl derivatives of 7-oxo-8-ribitylaminolumazines as inhibitors of riboflavin synthase and lumazine synthase". J. Org. Chem. 67 (16): 5807–5816. doi:10.1021/jo0201631. PMID 12153285. 
  11. ^ Haase, I., Mortl, S., Kohler, P., Bacher, A. and Fischer, M. (2003). "Biosynthesis of riboflavin in archaea. 6,7-dimethyl-8-ribityllumazine synthase of Methanococcus jannaschii". Eur. J. Biochem. 270 (5): 1025–1032. doi:10.1046/j.1432-1033.2003.03478.x. PMID 12603336. 
  12. ^ Morgunova, E., Meining, W., Illarionov, B., Haase, I., Jin, G., Bacher, A., Cushman, M., Fischer, M. and Ladenstein, R. (2005). "Crystal structure of lumazine synthase from Mycobacterium tuberculosis as a target for rational drug design: binding mode of a new class of purinetrione inhibitors". Biochemistry 44 (8): 2746–2758. doi:10.1021/bi047848a. PMID 15723519. 
  13. ^ Morgunova, E., Saller, S., Haase, I., Cushman, M., Bacher, A., Fischer, M. and Ladenstein, R. (2007). "Lumazine synthase from Candida albicans as an anti-fungal target enzyme: structural and biochemical basis for drug design". J. Biol. Chem. 282 (23): 17231–17241. doi:10.1074/jbc.M701724200. PMID 17446177. 
  14. ^ Fornasari MS, Laplagne DA, Frankel N, Cauerhff AA, Goldbaum FA, Echave J (2004). "Sequence determinants of quaternary structure in lumazine synthase". Mol. Biol. Evol. 21 (1): 97–107. doi:10.1093/molbev/msg244. PMID 14523158. 

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