Lumazine synthase

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6,7-dimethyl-8-ribityllumazine synthase
Identifiers
EC number 2.5.1.78
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum

Lumazine synthase (EC 2.5.1.78, 6,7-dimethyl-8-ribityllumazine synthase, 6,7-dimethyl-8-ribityllumazine synthase 2, 6,7-dimethyl-8-ribityllumazine synthase 1, lumazine synthase 2, lumazine synthase 1, type I lumazine synthase, type II lumazine synthase, RIB4, MJ0303, RibH, Pbls, MbtLS, RibH1 protein, RibH2 protein, RibH1, RibH2) is an enzyme with system name 5-amino-6-(D-ribitylamino)uracil butanedionetransferase.[1][2][3][4][5][6][7][8][9][10][11][12][13][14] This enzyme catalyses the following chemical reaction

1-deoxy-L-glycero-tetrulose 4-phosphate + 5-amino-6-(D-ribitylamino)uracil \rightleftharpoons 6,7-dimethyl-8-(D-ribityl)lumazine + 2 H2O + phosphate

This enzyme is involved in riboflavin biosynthesis.

References[edit]

  1. ^ Kis, K., Volk, R. and Bacher, A. (1995). "Biosynthesis of riboflavin. Studies on the reaction mechanism of 6,7-dimethyl-8-ribityllumazine synthase". Biochemistry 34 (9): 2883–2892. doi:10.1021/bi00009a019. PMID 7893702. 
  2. ^ Garcia-Ramirez, J.J., Santos, M.A. and Revuelta, J.L. (1995). "The Saccharomyces cerevisiae RIB4 gene codes for 6,7-dimethyl-8-ribityllumazine synthase involved in riboflavin biosynthesis. Molecular characterization of the gene and purification of the encoded protein". J. Biol. Chem. 270 (40): 23801–23807. doi:10.1074/jbc.270.40.23801. PMID 7559556. 
  3. ^ Bacher, A., Fischer, M., Kis, K., Kugelbrey, K., Mörtl, S., Scheuring, J., Weinkauf, S., Eberhardt, S., Schmidt-Bäse, K., Huber, R., Ritsert, K., Cushman, M., Ladenstein, R. (1996). "Biosynthesis of riboflavin: structure and mechanism of lumazine synthase". Biochem. Soc. Trans. 24 (1): 89–94. PMID 8674771. 
  4. ^ Mörtl, S., Fischer, M., Richter, G., Tack, J., Weinkauf, S. and Bacher, A. (1996). "Biosynthesis of riboflavin. Lumazine synthase of Escherichia coli". J. Biol. Chem. 271 (52): 33201–33207. doi:10.1074/jbc.271.52.33201. PMID 8969176. 
  5. ^ Bacher, A., Eberhardt, S., Fischer, M., Mortl, S., Kis, K., Kugelbrey, K., Scheuring, J. and Schott, K. (1997). "Biosynthesis of riboflavin: lumazine synthase and riboflavin synthase". Methods Enzymol. Methods in Enzymology 280: 389–399. doi:10.1016/S0076-6879(97)80130-9. ISBN 9780121821814. PMID 9211334. 
  6. ^ Goldbaum, F.A., Velikovsky, C.A., Baldi, P.C., Mortl, S., Bacher, A. and Fossati, C.A. (1999). "The 18-kDa cytoplasmic protein of Brucella species - an antigen useful for diagnosis - is a lumazine synthase". J. Med. Microbiol. 48 (9): 833–839. doi:10.1099/00222615-48-9-833. PMID 10482294. 
  7. ^ Jordan, D.B., Bacot, K.O., Carlson, T.J., Kessel, M. and Viitanen, P.V. (1999). "Plant riboflavin biosynthesis. Cloning, chloroplast localization, expression, purification, and partial characterization of spinach lumazine synthase". J. Biol. Chem. 274 (31): 22114–22121. doi:10.1074/jbc.274.31.22114. PMID 10419541. 
  8. ^ Zhang, X., Meining, W., Fischer, M., Bacher, A. and Ladenstein, R. (2001). "X-ray structure analysis and crystallographic refinement of lumazine synthase from the hyperthermophile Aquifex aeolicus at 1.6 Å resolution: determinants of thermostability revealed from structural comparisons". J. Mol. Biol. 306 (5): 1099–1114. doi:10.1006/jmbi.2000.4435. PMID 11237620. 
  9. ^ Fischer, M., Haase, I., Feicht, R., Richter, G., Gerhardt, S., Changeux, J.P., Huber, R. and Bacher, A. (2002). "Biosynthesis of riboflavin: 6,7-dimethyl-8-ribityllumazine synthase of Schizosaccharomyces pombe". Eur. J. Biochem. 269 (2): 519–526. doi:10.1046/j.0014-2956.2001.02674.x. PMID 11856310. 
  10. ^ Cushman, M., Yang, D., Gerhardt, S., Huber, R., Fischer, M., Kis, K. and Bacher, A. (2002). "Design, synthesis, and evaluation of 6-carboxyalkyl and 6-phosphonoxyalkyl derivatives of 7-oxo-8-ribitylaminolumazines as inhibitors of riboflavin synthase and lumazine synthase". J. Org. Chem. 67 (16): 5807–5816. doi:10.1021/jo0201631. PMID 12153285. 
  11. ^ Haase, I., Mortl, S., Kohler, P., Bacher, A. and Fischer, M. (2003). "Biosynthesis of riboflavin in archaea. 6,7-dimethyl-8-ribityllumazine synthase of Methanococcus jannaschii". Eur. J. Biochem. 270 (5): 1025–1032. doi:10.1046/j.1432-1033.2003.03478.x. PMID 12603336. 
  12. ^ Morgunova, E., Meining, W., Illarionov, B., Haase, I., Jin, G., Bacher, A., Cushman, M., Fischer, M. and Ladenstein, R. (2005). "Crystal structure of lumazine synthase from Mycobacterium tuberculosis as a target for rational drug design: binding mode of a new class of purinetrione inhibitors". Biochemistry 44 (8): 2746–2758. doi:10.1021/bi047848a. PMID 15723519. 
  13. ^ Morgunova, E., Saller, S., Haase, I., Cushman, M., Bacher, A., Fischer, M. and Ladenstein, R. (2007). "Lumazine synthase from Candida albicans as an anti-fungal target enzyme: structural and biochemical basis for drug design". J. Biol. Chem. 282 (23): 17231–17241. doi:10.1074/jbc.M701724200. PMID 17446177. 
  14. ^ Fornasari MS, Laplagne DA, Frankel N, Cauerhff AA, Goldbaum FA, Echave J (2004). "Sequence determinants of quaternary structure in lumazine synthase". Mol. Biol. Evol. 21 (1): 97–107. doi:10.1093/molbev/msg244. PMID 14523158. 

External links[edit]