M17 agar

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This bacterial growth medium was developed in 1971 for Lactococcus species isolated from milk products. It was originally called M16 medium,[1] but in 1975 Terzaghi and Sandine[2] added disodium-β-glycerophosphate to the medium as a buffer, and named the new growth medium M17 medium. It was later found that the addition of disodium-β-glycerophosphate inhibits the growth of many Lactobacillus species[3]

Typical composition[edit]

Per 950 ml:[4]

Preparation:

  • 1. Heat with frequent agitation and boil for 1 minute to completely dissolve.
  • 2. Autoclave at 121°C for 15 minutes. Cool to 50°C.
  • 3. Add 50 ml filter sterilized 10% lactose solution and mix well (the lactose can be exchanged to other carbohydrates e.g. glucose, resulting in GM17 medium)

References[edit]

  1. ^ Lowrie, R. J., and L. E. Pearce. (1971). The plating efficiency of bacteriophages of lactic streptococci. N.Z. J. Dairy Sci. Technol. 6:166-171.
  2. ^ Terzaghi B. E., and W. E. Sandine. (1975 ). Improved medium for lactic streptococci and their bacteriophages. Appl Microbiol. Jun;29(6):807-13. PubMed PMID 16350018
  3. ^ Shankar, P. A., and F. L. Davies. (1977). A note on the suppression of Lactobacillus bulgaricus in media containing 0-glycerophosphate and application of the media to selective isolation of Streptococcus thermophilus from yoghurt. J. Soc. Dairy Technol. 30( 1 ): 28.
  4. ^ Terzaghi B. E., and W. E. Sandine. (1975 ). Improved medium for lactic streptococci and their bacteriophages. Appl Microbiol. Jun;29(6):807-13. PubMed PMID 16350018