Culture of rat brain cells stained with antibody to MAP2 (green), Neurofilament NF-H (red) and DNA (blue). MAP2 is found in neuronal dendrites, while the neurofilament is found predominantly in axons. Antibodies and image courtesy of EnCor Biotechnology
Microtubule-associated protein 2 is a protein that in humans is encoded by the MAP2gene.
This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neuritogenesis. MAP2 serves to stabilize microtubules (MT) growth by crosslinking MT with intermediate filaments and other MT. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dendrites, implicating a role in determining and stabilizing dendritic shape during neuron development. A number of alternatively spliced variants encoding distinct isforms have been described.
^Neve RL, Harris P, Kosik KS, Kurnit DM, Donlon TA (May 1987). "Identification of cDNA clones for the human microtubule-associated protein tau and chromosomal localization of the genes for tau and microtubule-associated protein 2". Brain Res387 (3): 271–80. PMID3103857.
^Lim, R W; Halpain S (July 2000). "Regulated association of microtubule-associated protein 2 (MAP2) with Src and Grb2: evidence for MAP2 as a scaffolding protein". J. Biol. Chem. (UNITED STATES) 275 (27): 20578–87. doi:10.1074/jbc.M001887200. ISSN0021-9258. PMID10781592.Cite uses deprecated parameters (help)
^Zamora-Leon, S P; Lee G, Davies P, Shafit-Zagardo B (October 2001). "Binding of Fyn to MAP-2c through an SH3 binding domain. Regulation of the interaction by ERK2". J. Biol. Chem. (United States) 276 (43): 39950–8. doi:10.1074/jbc.M107807200. ISSN0021-9258. PMID11546790.Cite uses deprecated parameters (help)
Roses AD, Einstein G, Gilbert J, et al. (1996). "Morphological, biochemical, and genetic support for an apolipoprotein E effect on microtubular metabolism". Ann. N. Y. Acad. Sci.777: 146–57. doi:10.1111/j.1749-6632.1996.tb34413.x. PMID8624078.
Shafit-Zagardo B, Kalcheva N (1999). "Making sense of the multiple MAP-2 transcripts and their role in the neuron". Mol. Neurobiol.16 (2): 149–62. doi:10.1007/BF02740642. PMID9588626.
Liu Y, Saad RS, Shen SS, Silverman JF (2003). "Diagnostic value of microtubule-associated protein-2 (MAP-2) for neuroendocrine neoplasms". Advances in anatomic pathology10 (2): 101–6. doi:10.1097/00125480-200303000-00005. PMID12605092.
Dammerman M, Yen SH, Shafit-Zagardo B (1990). "Sequence of a human MAP-2 region sharing epitopes with Alzheimer neurofibrillary tangles". J. Neurosci. Res.24 (4): 487–95. doi:10.1002/jnr.490240405. PMID2481044.
Obar RA, Dingus J, Bayley H, Vallee RB (1990). "The RII subunit of cAMP-dependent protein kinase binds to a common amino-terminal domain in microtubule-associated proteins 2A, 2B, and 2C". Neuron3 (5): 639–45. doi:10.1016/0896-6273(89)90274-2. PMID2561973.
Rubino HM, Dammerman M, Shafit-Zagardo B, Erlichman J (1990). "Localization and characterization of the binding site for the regulatory subunit of type II cAMP-dependent protein kinase on MAP2". Neuron3 (5): 631–8. doi:10.1016/0896-6273(89)90273-0. PMID2701845.
Herrmann H, Wiche G (1987). "Plectin and IFAP-300K are homologous proteins binding to microtubule-associated proteins 1 and 2 and to the 240-kilodalton subunit of spectrin". J. Biol. Chem.262 (3): 1320–5. PMID3027087.
Garner CC, Tucker RP, Matus A (1989). "Selective localization of messenger RNA for cytoskeletal protein MAP2 in dendrites". Nature336 (6200): 674–7. doi:10.1038/336674a0. PMID3200318.
Albala JS, Kalcheva N, Shafit-Zagardo B (1994). "Characterization of the transcripts encoding two isoforms of human microtubule-associated protein-2 (MAP-2)". Gene136 (1–2): 377–8. doi:10.1016/0378-1119(93)90502-T. PMID8294038.
Illenberger S, Drewes G, Trinczek B, et al. (1996). "Phosphorylation of microtubule-associated proteins MAP2 and MAP4 by the protein kinase p110mark. Phosphorylation sites and regulation of microtubule dynamics". J. Biol. Chem.271 (18): 10834–43. doi:10.1074/jbc.271.18.10834. PMID8631898.
Björkblom B, Ostman N, Hongisto V, et al. (2005). "Constitutively active cytoplasmic c-Jun N-terminal kinase 1 is a dominant regulator of dendritic architecture: role of microtubule-associated protein 2 as an effector". J Neurosci.25 (27): 6350–61. doi:10.1523/JNEUROSCI.1517-05.2005. PMID16000625.