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Minichromosome maintenance complex component 4
Symbols MCM4 ; CDC21; CDC54; NKCD; NKGCD; P1-CDC21; hCdc21
External IDs OMIM602638 MGI103199 HomoloGene40496 GeneCards: MCM4 Gene
EC number
RNA expression pattern
PBB GE MCM4 212141 at tn.png
PBB GE MCM4 212142 at tn.png
PBB GE MCM4 222036 s at tn.png
More reference expression data
Species Human Mouse
Entrez 4173 17217
Ensembl ENSG00000104738 ENSMUSG00000022673
UniProt P33991 P49717
RefSeq (mRNA) NM_005914 NM_008565
RefSeq (protein) NP_005905 NP_032591
Location (UCSC) Chr 8:
48.87 – 48.89 Mb
Chr 16:
15.62 – 15.64 Mb
PubMed search [1] [2]

DNA replication licensing factor MCM4 is a protein that in humans is encoded by the MCM4 gene.[1]

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported.[2]

See also[edit]


MCM4 has been shown to interact with ORC1L,[3] ORC2L,[3] Replication protein A1,[3] ORC4L,[3] ORC5L,[3] ORC3L,[3] MCM6,[3][4][5][6] MCM7,[4][5][6][7] MCM2,[3][4][5][8] Cell division cycle 7-related protein kinase[3] and ORC6L.[3]


  1. ^ Musahl C, Schulte D, Burkhart R, Knippers R (August 1995). "A human homologue of the yeast replication protein Cdc21. Interactions with other Mcm proteins". Eur J Biochem 230 (3): 1096–101. doi:10.1111/j.1432-1033.1995.tb20660.x. PMID 7601140. 
  2. ^ "Entrez Gene: MCM4 MCM4 minichromosome maintenance deficient 4 (S. cerevisiae)". 
  3. ^ a b c d e f g h i j Kneissl, Margot; Pütter Vera; Szalay Aladar A; Grummt Friedrich (March 2003). "Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells". J. Mol. Biol. (England) 327 (1): 111–28. doi:10.1016/S0022-2836(03)00079-2. ISSN 0022-2836. PMID 12614612. 
  4. ^ a b c Yabuta, Norikazu; Kajimura Naoko; Mayanagi Kouta; Sato Michio; Gotow Takahito; Uchiyama Yasuo; Ishimi Yukio; Nojima Hiroshi (May 2003). "Mammalian Mcm2/4/6/7 complex forms a toroidal structure". Genes Cells (England) 8 (5): 413–21. doi:10.1046/j.1365-2443.2003.00645.x. ISSN 1356-9597. PMID 12694531. 
  5. ^ a b c Ishimi, Y; Ichinose S; Omori A; Sato K; Kimura H (September 1996). "Binding of human minichromosome maintenance proteins with histone H3". J. Biol. Chem. (UNITED STATES) 271 (39): 24115–22. doi:10.1074/jbc.271.39.24115. ISSN 0021-9258. PMID 8798650. 
  6. ^ a b You, Zhiying; Ishimi Yukio; Masai Hisao; Hanaoka Fumio (November 2002). "Roles of Mcm7 and Mcm4 subunits in the DNA helicase activity of the mouse Mcm4/6/7 complex". J. Biol. Chem. (United States) 277 (45): 42471–9. doi:10.1074/jbc.M205769200. ISSN 0021-9258. PMID 12207017. 
  7. ^ Fujita, M; Kiyono T; Hayashi Y; Ishibashi M (April 1997). "In vivo interaction of human MCM heterohexameric complexes with chromatin. Possible involvement of ATP". J. Biol. Chem. (UNITED STATES) 272 (16): 10928–35. doi:10.1074/jbc.272.16.10928. ISSN 0021-9258. PMID 9099751. 
  8. ^ You, Z; Komamura Y; Ishimi Y (December 1999). "Biochemical Analysis of the Intrinsic Mcm4-Mcm6-Mcm7 DNA Helicase Activity". Mol. Cell. Biol. (UNITED STATES) 19 (12): 8003–15. ISSN 0270-7306. PMC 84885. PMID 10567526. 

Further reading[edit]