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MNAT CDK-activating kinase assembly factor 1
Protein MNAT1 PDB 1g25.png
PDB rendering based on 1g25.
Available structures
PDB Ortholog search: PDBe, RCSB
Symbols MNAT1 ; CAP35; MAT1; RNF66; TFB3
External IDs OMIM602659 MGI106207 HomoloGene1821 ChEMBL: 5053 GeneCards: MNAT1 Gene
RNA expression pattern
PBB GE MNAT1 203565 s at tn.png
More reference expression data
Species Human Mouse
Entrez 4331 17420
Ensembl ENSG00000020426 ENSMUSG00000021103
UniProt P51948 P51949
RefSeq (mRNA) NM_001177963 NM_008612
RefSeq (protein) NP_001171434 NP_032638
Location (UCSC) Chr 14:
61.2 – 61.44 Mb
Chr 12:
73.12 – 73.27 Mb
PubMed search [1] [2]

CDK-activating kinase assembly factor MAT1 is an enzyme that in humans is encoded by the MNAT1 gene.[1]

Cyclin-dependent kinases (CDKs), which play an essential role in cell cycle control of eukaryotic cells, are phosphorylated and thus activated by the CDK-activating kinase (CAK). CAK is a multisubunit protein that includes CDK7 (MIM 601955), cyclin H (CCNH; MIM 601953), and MAT1. MAT1 (for 'menage a trois-1') is involved in the assembly of the CAK complex.[supplied by OMIM][2]


MNAT1 has been shown to interact with POU2F1,[3] MTA1,[4] Cyclin H,[4][5] P53,[6] Estrogen receptor alpha,[4] MCM7[7] and Cyclin-dependent kinase 7.[4][8]


  1. ^ Eki T, Okumura K, Abe M, Kagotani K, Taguchi H, Murakami Y, Pan ZQ, Hanaoka F (April 1998). "Mapping of the human genes encoding cyclin H (CCNH) and the CDK-activating kinase (CAK) assembly factor MAT1 (MNAT1) to chromosome bands 5q13.3-q14 and 14q23, respectively". Genomics 47 (1): 115–20. doi:10.1006/geno.1997.5053. PMID 9465303. 
  2. ^ "Entrez Gene: MNAT1 menage a trois homolog 1, cyclin H assembly factor (Xenopus laevis)". 
  3. ^ Inamoto, S; Segil N; Pan Z Q; Kimura M; Roeder R G (November 1997). "The cyclin-dependent kinase-activating kinase (CAK) assembly factor, MAT1, targets and enhances CAK activity on the POU domains of octamer transcription factors". J. Biol. Chem. (UNITED STATES) 272 (47): 29852–8. doi:10.1074/jbc.272.47.29852. ISSN 0021-9258. PMID 9368058. 
  4. ^ a b c d Talukder, Amjad H; Mishra Sandip K; Mandal Mahitosh; Balasenthil Seetharaman; Mehta Sonal; Sahin Aysegul A; Barnes Christopher J; Kumar Rakesh (March 2003). "MTA1 interacts with MAT1, a cyclin-dependent kinase-activating kinase complex ring finger factor, and regulates estrogen receptor transactivation functions". J. Biol. Chem. (United States) 278 (13): 11676–85. doi:10.1074/jbc.M209570200. ISSN 0021-9258. PMID 12527756. 
  5. ^ Ewing, Rob M; Chu Peter, Elisma Fred, Li Hongyan, Taylor Paul, Climie Shane, McBroom-Cerajewski Linda, Robinson Mark D, O'Connor Liam, Li Michael, Taylor Rod, Dharsee Moyez, Ho Yuen, Heilbut Adrian, Moore Lynda, Zhang Shudong, Ornatsky Olga, Bukhman Yury V, Ethier Martin, Sheng Yinglun, Vasilescu Julian, Abu-Farha Mohamed, Lambert Jean-Philippe, Duewel Henry S, Stewart Ian I, Kuehl Bonnie, Hogue Kelly, Colwill Karen, Gladwish Katharine, Muskat Brenda, Kinach Robert, Adams Sally-Lin, Moran Michael F, Morin Gregg B, Topaloglou Thodoros, Figeys Daniel (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. (England) 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931. 
  6. ^ Ko, L J; Shieh S Y; Chen X; Jayaraman L; Tamai K; Taya Y; Prives C; Pan Z Q (December 1997). "p53 is phosphorylated by CDK7-cyclin H in a p36MAT1-dependent manner". Mol. Cell. Biol. (UNITED STATES) 17 (12): 7220–9. ISSN 0270-7306. PMC 232579. PMID 9372954. 
  7. ^ Wang, Y; Xu F; Hall F L (October 2000). "The MAT1 cyclin-dependent kinase-activating kinase (CAK) assembly/targeting factor interacts physically with the MCM7 DNA licensing factor". FEBS Lett. (NETHERLANDS) 484 (1): 17–21. doi:10.1016/S0014-5793(00)02117-7. ISSN 0014-5793. PMID 11056214. 
  8. ^ Yee, A; Nichols M A; Wu L; Hall F L; Kobayashi R; Xiong Y (December 1995). "Molecular cloning of CDK7-associated human MAT1, a cyclin-dependent kinase-activating kinase (CAK) assembly factor". Cancer Res. (UNITED STATES) 55 (24): 6058–62. ISSN 0008-5472. PMID 8521393. 

Further reading[edit]