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MSF may refer to:
- MSF, the United States Navy hull classification symbol for "fleet minesweeper"
- Mail Summary File (.msf), file extension used by Earthlink, Mozilla Thunderbird, and Netscape mail clients to store folder data in Mork.
- Marvel Super Heroes vs. Street Fighter, a fighting game by Capcom featuring Marvel Comics and Street Fighter characters
- Frankenstein (1994 film), also known as Mary Shelley's Frankenstein
- Master of Science in Finance
- Metasploit Framework an open-source computer security project which provides information about security vulnerabilities and aids in penetration testing and IDS signature development
- Methanesulfonyl fluoride
- Microsoft SharePoint Foundation, formerly known as Windows SharePoint Services
- Microsoft Solutions Framework
- Military science fiction
- Multi-Stage Flash, a water desalination process
- Municipal solid waste, garbage
- Time from NPL, UK National Physical Laboratory time signal (which historically used the MSF radio call sign)
- Masonic Samaritan Fund, a UK charity catering for the medical needs of Masons and their dependents, formerly known as the New Masonic Samaritan Fund.
- Manufacturing, Science and Finance, a UK trade union (now part of Unite)
- Maryland Shakespeare Festival, a theater company
- Mathematical Sciences Foundation, an Indian institution for education and research
- MedcomSoft, a medical office automation software company
- Médecins Sans Frontières (aka Doctors Without Borders) is a non-profit medical and humanitarian organisation
- Melbourne School of Fashion
- Militaires Sans Frontieres (Soldiers Without Borders), a fictional mercenary group from the Metal Gear Solid game franchise
- Mind Science Foundation
- Motorcycle Safety Foundation, the US organization that promotes motorcycle safety
- Muhammad Subuh Foundation, a Subud charitable foundation
- Multiservice Switching Forum, also sometimes MultiService Forum, a telecommunications industry association
- Muslim Students Federation in India
- Muslim Safety Forum, a UK organisation challenging the "unfair focus on the Muslim community when it came to policing activities and enforcement of anti-terror policing legislation"
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