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MSF may refer to:
- MSF, the United States Navy hull classification symbol for "fleet minesweeper"
- Mail Summary File (.msf), file extension used by Earthlink, Mozilla Thunderbird, and Netscape mail clients to store folder data in Mork.
- Master of Science in Finance
- Methanesulfonyl fluoride
- Microsoft Solutions Framework
- Multi-Stage Flash, a water desalination process
- MSF, former call sign of Time from NPL
- Manufacturing, Science and Finance, a UK trade union (now part of Unite)
- Maryland Shakespeare Festival, a theater company
- Mathematical Sciences Foundation, an Indian institution for education and research
- Médecins Sans Frontières (aka Doctors Without Borders), humanitarian-aid non-governmental organization
- Melbourne School of Fashion
- Militaires Sans Frontieres (Soldiers Without Borders), mercenary group in Metal Gear Solid video games
- Mind Science Foundation
- Motorcycle Safety Foundation, the US organization that promotes motorcycle safety
- Muhammad Subuh Foundation, a Subud charitable foundation
- Multiservice Switching Forum or MultiService Forum, a telecommunications industry association
- Muslim Students Federation in India
- Muslim Safety Forum, a UK organisation
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