With a family resemblance to the military twin-engine M.C.99 and earlier M.C.94 the M.C.100 was a shoulder-wing cantilevermonoplane flying boat. It was powered by three Alfa Romeo 126 RC 10 radial engines strut mounted above the wing with each driving a three-bladed tractor propeller. The pilot and co-pilot sat side by side in a raised and enclosed control cabin forward of the wing while the radio operator sat in the aircraft's nose. A main cabin in the hull had accommodation for 26 passengers. The prototype first flew on 7 January 1939. The prototype was followed by two more aircraft, and all three were in service by June 1940 with Ala Littoria operating between Rome-Algiers-Barcelona. With the start of World War II, the aircraft was used for liaison and communication duties and to maintain a daily Rome-Marsala-Tripoli service.