Maggot therapy

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A wound cleaned by maggots

Maggot therapy is also known as maggot debridement therapy (MDT), larval therapy, larva therapy, larvae therapy, biodebridement or biosurgery. It is a type of biotherapy involving the introduction of live, disinfected maggots (fly larvae) into the non-healing skin and soft tissue wound(s) of a human or animal for the purpose of cleaning out the necrotic (dead) tissue within a wound (debridement) and disinfection. It was long believed that the debridement is selective on necrotic tissue but new literature first questioned that belief.[1] A recent clinical study in France showed non-selectivity of maggot action as wound surface increased over treatment.[2]

History[edit]

Tales and early history[edit]

Written records have documented that maggots have been used since antiquity as a wound treatment.[3] There are reports of the use of maggots for wound healing by Maya Native Americans and Aboriginal tribes in Australia. There also have been reports of the use of maggot treatment in Renaissance times. Military physicians have observed that soldiers whose wounds had become colonized with maggots experienced significantly less morbidity and mortality than soldiers whose wounds had not become colonized. These physicians included Napoleon’s general surgeon, Baron Dominique Larrey. Larrey reported during France's Egyptian campaign in Syria, 1798–1801, that certain species of fly consumed only dead tissue and helped wounds to heal.[4]

Dr. Joseph Jones, a ranking Confederate medical officer during the American Civil War, is quoted as follows, "I have frequently seen neglected wounds ... filled with maggots ... as far as my experience extends, these worms eat Zacharias, who reported during the American Civil War that, "Maggots ... in a single day would clean a wound much better than any agents we had at our command ... I am sure I saved many lives by their use." He recorded a high survival rate in patients he treated with maggots.[5]

During World War I, Dr. William S. Baer, an orthopedic surgeon, recognized on the battlefield the efficacy of maggot colonization for healing wounds. He observed one soldier left for several days on the battlefield who had sustained compound fractures of the femur and large flesh wounds of the abdomen and scrotum. When the soldier arrived at the hospital, he had no signs of fever despite the serious nature of his injuries and his prolonged exposure to the elements without food or water. When his clothes were removed, it was seen that "thousands and thousands of maggots filled the entire wounded area." To Dr. Baer's surprise, when these maggots were removed "there was practically no bare bone to be seen and the internal structure of the wounded bone as well as the surrounding parts was entirely covered with most beautiful pink tissue that one could imagine." This case took place at a time when the death rate for compound fractures of the femur was about 75–80%.[citation needed]

Case studies and modern use[edit]

While at Johns Hopkins University in 1929, Dr. Baer introduced maggots into 21 patients with intractable chronic osteomyelitis. He observed rapid debridement, reductions in the number of pathogenic organisms, reduced odor levels, alkalinization of wound beds, and ideal rates of healing. All 21 patients' open lesions were completely healed and they were released from the hospital after two months of maggot therapy.

After the publication of Dr. Baer's results in 1931,[6] maggot therapy for wound care became very common, particularly in the United States. The Lederle pharmaceutical company commercially produced "Surgical Maggots", larvae of the green bottle fly, which primarily feed on the necrotic (dead) tissue of the living host without attacking living tissue. Between 1930 and 1940, more than 100 medical papers were published on maggot therapy. Medical literature of this time contains many references to the successful use of maggots in chronic or infected wounds including osteomyelitis, abscesses, burns, sub-acute mastoiditis,[7][8] and chronic empyema.[9]

More than 300 American hospitals employed maggot therapy during the 1940s. The extensive use of maggot therapy prior to World War II was curtailed when the discovery and growing use of penicillin caused it to be deemed outdated. Due to the lack of conventional medicines, maggot therapy was used by Allied military medical staff in Japanese prisoner of war camps in the Far East throughout World War II.[10]

Reintroduction – more case studies[edit]

With the advent of antibiotic-resistant bacteria, Dr. Ronald Sherman, a physician previously at the University of California, Irvine, sought to re-introduce maggot therapy into the armamentarium of modern medical care. In 1989, he set up fly breeding facilities at the Veterans Affairs Medical Center in Long Beach, California in order to use maggots for the treatment of wounds. Using a Paralyzed Veterans of America grant, he initiated a prospective controlled clinical trial of maggot therapy for spinal cord patients with pressure ulcers who had failed two or more courses of conventional wound care.[11]

Over 50 scientific papers have been published that describe the medical use of maggots. Six thousand maggot therapy patients have been included in case histories or other studies. About 400 patients have been documented within clinical studies, most of them are included in a British study financed by the NHS.[12]

Limb salvage rates with maggot therapy are about 40% to 50% according to the medical literature. Some report success rates of 70% to 80%, though definitions of "success" can vary.[13]

In a 2007 preliminary trial, maggots were used successfully to treat patients whose wounds were infected with MRSA, a bacterium (Staphylococcus aureus) with resistance to most antibiotics, including methicillin. Some of these strains include flesh eating bacteria causing frequent deaths upon infection of deep tissue. Maggots clean up the already dead tissue thus preventing further infection spread.[14]

In 1995, a handful of doctors in four countries were using maggot therapy. Today, any physician in the U.S. can prescribe maggot therapy. There are over 800 health care centers in the United States that have utilized maggot therapy. Over 4,000 therapists are using maggot therapy in 20 countries. Approximately 50,000 treatments were applied to wounds in the year 2006.[citation needed] These data appear on the website of the Wound Care Information Network, but the original sources are not provided.[15]

Regulation[edit]

In January 2004, the U.S. Food and Drug Administration (FDA) granted permission to produce and market maggots for use in humans or animals as a prescription-only medical device for the following indications:[16][17] "For debriding non-healing necrotic skin and soft tissue wounds, including pressure ulcers, venous stasis ulcers, neuropathic foot ulcers, and non-healing traumatic or post-surgical wounds." In February 2004, the British National Health Service (NHS) permitted its doctors to prescribe maggot therapy. In the European Union, Canada and Japan maggots are classified as medicinal drugs, needing a full market licence. In the U.S., maggots for medicinal use are classified as a device.

Veterinary use[edit]

The use of maggots to clean dead tissue from animal wounds is part of folk medicine[18] in many parts of the world. It is particularly helpful with chronic osteomyelitis, chronic ulcers, and other pus-producing infections that are frequently caused by chafing due to work equipment. Maggot therapy for horses in the United States was re-introduced after a study published in 2003 by veterinarian Dr. Scott Morrison. This therapy is used in horses for conditions such as osteomyelitis secondary to laminitis, sub-solar abscesses leading to osteomyelitis, post-surgical treatment of street-nail procedure for puncture wounds infecting the navicular bursa, canker, non-healing ulcers on the frog, and post-surgical site cleaning for keratoma removal.[19]

However, there have not been many case studies done with maggot debridement therapy on animals, therefore, it can be difficult to assess how successful it is (Jones & Wall, 2007).

Application of maggot wound dressings[edit]

Maggots are contained in a cage-like dressing over the wound for two days. The maggots may be allowed to move freely within that cage, with the wound floor acting as the bottom of the cage; or the maggots may be contained within a sealed pouch, placed on top of the wound. The dressing must be kept air permeable because maggots require oxygen to live. When maggots are satiated, they become substantially larger and seek to leave the site of a wound. Multiple two-day courses of maggot therapy may be administered depending on the severity of the non-healing wound.[20]

Maggots can never reproduce in the wound since they are still in the larval stage and too immature to do so. Reproduction can only occur when they become adult flies and mate.

Mechanisms of action[edit]

The maggots have one principal action reported in the medical literature:

  • debride wounds by dissolving necrotic (dead), infected tissue.[12] as well as vital tissue when dose is too high [1] or time of treatment is not appropriate [2]

Disinfection of the wound by killing bacteria and healing could not be proved by the largest clinical study available.[12]

Maggot therapy may also reduce the need for antibiotics in people with complex, chronic wounds.[21]

Maggot therapy has been shown to accelerate debridement of necrotic wounds. In about 1/3 of all patients pain is increased.[12]

Maggot therapy is further compatible with other wound care therapies such as antibiotics, negative pressure wound therapy (NPWT), skin grafting and hyperbaric oxygen therapy. While maggot therapy cannot be used simultaneously with NPWT, it can be used prior to NPWT to debride a wound so that it can be later closed with NPWT. Similarly, while maggot therapy cannot be used simultaneously with skin grafting, it can be used prior to skin grafting to debride a wound so that it can be later closed with skin grafting.

Debridement[edit]

The debridement of necrotic (dead) tissue using maggots or other methods has not been proven to provide medical benefit compared to no debridement.[22][23]

In maggot therapy, large numbers of small maggots consume necrotic tissue far more precisely than is possible in a normal surgical operation, and can debride a wound in a day or two. While in the past it was believed that maggots do not damage healthy tissue, this is in doubt now.[24] Wound surface is increased on the use of maggots.[2] They derive nutrients through a process known as "extracorporeal digestion" by secreting a broad spectrum of proteolytic enzymes[25] that liquefy necrotic tissue, and absorb the semi-liquid result within a few days. In an optimum wound environment maggots molt twice, increasing in length from 1–2 mm to 8–10 mm, and in girth, within a period of 3–4 days by ingesting necrotic tissue, leaving a clean wound free of necrotic tissue when they are removed. When they stay longer or too many are used, healthy tissue is removed as well.[2][24]

Disinfection[edit]

Any wound infection is always a serious medical complication. Infected living tissue cannot heal. If the wound is infected with an antibiotic-resistant bacterial strain, it becomes difficult or impossible to treat the underlying infection and for any healing to occur. Wound infection could further be limb- and life-threatening. When maggots successfully debride a necrotic wound, the source of wound infection is usually removed; however, a health technology assessment conducted by the Department of Health Sciences of University of York found no statistically significant evidence for this.[23]

Maggot secretions are also effective against some antibiotic-resistant bacteria and have been shown to possess potent antimicrobial activity in early experimental studies from the 1930s.[citation needed] As early as 1957, a specific antibiotic factor was found in maggot secretions and published in the journal Nature.[26] Secretions believed to have broad-spectrum antimicrobial activity include allantoin, urea, phenylacetic acid, phenylacetaldehyde, calcium carbonate, proteolytic enzymes, and many others.[27] Bacteria not killed by these secretions are subsequently ingested and lysed within the maggots.[citation needed]

In vitro studies have shown that maggots inhibit and destroy a wide range of pathogenic bacteria including methicillin-resistant Staphylococcus aureus (MRSA), group A and B streptococci, and Gram-positive aerobic and anaerobic strains.[citation needed] Therefore maggot therapy might represent a cost-effective method for managing MRSA infection.[citation needed] Other bacteria like Pseudomonas aeruginosa, E.coli or Proteus spec are not attacked by maggots and in case of Pseudomonas even the maggots are in danger.[28] Consequently, using maggots alone might lead to a change of bacteria cultures on the wound.

Wound healing[edit]

Maggot therapy has been expected from case studies to have wound healing properties. Two European clinical studies show that healing cannot be expected by maggots.[2][12][24]

Limitations[edit]

The wound must be of a type which can actually benefit from the application of maggot therapy. A moist, exudating wound with sufficient oxygen supply is a prerequisite. Not all wound-types are suitable: wounds which are dry, or open wounds of body cavities do not provide a good environment for maggots to feed. In some cases it may be possible to make a dry wound suitable for larval therapy by moistening it with saline soaks, applied for 48 hours.

Maggots have a short shelf life which prevents long term storage before use.[29] Patients and doctors may find maggots distasteful, although studies have shown that this does not cause patients to refuse the offer of maggot therapy.[30] Maggots can be enclosed in opaque polymer bags to hide them from sight. Dressings must be designed to prevent any maggots from escaping, while allowing air to get to the maggots.[31] Dressings are also designed to minimize the uncomfortable tickling sensation that the maggots often cause.[32]

Comparative studies[edit]

In 2008, a scientific study published in the British Medical Journal compared the merits of maggot therapy and standard hydrogels to treat 270 British patients with leg ulcers from around the UK.[12] Patients were treated with either maggots or hydrogel and their progress followed for up to a year.

The study revealed no significant differences in the time taken for the ulcer to heal, or in the patient's quality of life. Maggots were shown to be no more effective than hydrogel treatment at reducing the amount of bacteria present or in clearing MRSA. Although maggots were significantly more efficient at debridement of the wound, treatment with maggots was associated with more pain by patients. A separate study which compared the relative cost-effectiveness of maggot therapy with hydrogels estimated there was little to choose between the two therapies.[12][33]

In a 2011 study of people with non-healing wounds, maggot therapy was more effective in debriding than conventional surgical wound-cleaning during the first week, but there was no significant added benefit by day 15.[2] Surprisingly, wounds debrided by maggots showed significantly increased wound surface compared to surgically debridement "Moreover, the second week of treatment could be deleterious because a significant increase in wound surface area at day 15 in the MDT group was observed".[2]

Biology of flies and maggots used in maggot therapy[edit]

Lucilia sericata, Green Bottle Fly
Protophormia terraenovae, Northern blowfly

Maggots are fly larvae, or immature flies, just as caterpillars are butterfly or moth larvae. Not all species of flies are safe and effective as medicinal maggots. There are thousands of species of flies, each with its own habits and life cycle. Some fly larvae feed on plants or animals, or even blood. Others feed on rotting organic material.

Those flies whose larvae feed on dead animals will sometimes lay their eggs on the dead parts (necrotic or gangrenous tissue) of living animals. The infestation by maggots of live animals is called "myiasis." Some maggots will feed only on dead tissue, some only on live tissue, and some on live or dead tissue. The flies used most often for the purpose of maggot therapy are "blow flies" (Calliphoridae); and the species used most commonly is Lucilia sericata, the common green bottle fly. Another important species, Protophormia terraenovae, is also notable for its feeding secretions, which combat infection by Streptococcus pyogenes and Streptococcus pneumoniae.[34]

All maggots used for debridement are specialized and grown in a laboratory setting. This ensures that the larvae are sterilized in order to prevent secondary infections caused by microbes carried in by the maggots. Due to their short lifespan, usability is limited. This means that shipment must be direct and over night. They are also sensitive to temperature variations and must be transported with great care. Some of these challenges have limited the use of maggot therapy in certain regions of the world.

In popular culture[edit]

  • In Bernard Cornwell's novel Sharpe's Eagle, set in 1810, the wound of Lt. Sharpe is left clean by applied maggots. In its TV adaptation, rather than being merely discussed, the maggots are shown on the skin around the wound.
  • In an episode of Chicago Hope a patient undergoes maggot therapy. The maggots are not shown though.
  • In the 2000 film Gladiator, Russell Crowe's character has a shoulder wound cleaned with maggots.
  • In the young adult novel The Dolphins of Laurentum, taking place in Roman Italy, maggots were used on an injury as an alternative to amputation. In the story the therapy was successful and the man was able to walk again.
  • In addition, the television show House, M.D. included treatment of a burn by maggot therapy in the twelfth episode of the second season, titled "Distractions," on February 14, 2006.
  • Claire Fraser, a medical practitioner and principal character in Diana Gabaldon's Outlander series of novels, often uses maggots for debridement of necrotic tissue in her patients' wounds.
  • The titular character of Johnny Got His Gun claims that maggot therapy could have prevented his quadruple amputations.
  • In her 2009 novel The Year of the Flood, Margaret Atwood focuses on a group called God's Gardeners, a small community of survivors of the same environmental catastrophe who are using maggot therapy for many acute wounds.
  • In the American Dad season 9 episode "Killer Vacation", Steve mentions maggot therapy when his friend suggests using bees to clean his open wound.
  • In Ken Burns's 2007 WWII documentary The War, Earl Burke, a B17 ball turret gunner, describes maggot therapy debridement of his osteomyelitis infection from a wound sustained in action.
  • Dr. Gene Porter, small town doctor, father of Rachel Matheson in NBC's sci-fi drama Revolution uses maggots to help heal the infection in Miles's arm in episode s02e10.

References[edit]

  1. ^ a b http://www.bmj.com/content/338/bmj.b773?tab=responses
  2. ^ a b c d e f g Kristina Opletalová, Xavier Blaizot, Bénédicte Mourgeon, Yannick Chêne, Christian Creveuil, Patrick Combemale, Anne-Laure Laplaud, Ingrid Sohyer-Lebreuilly, Anne Dompmartin. 2011. Maggot Therapy for Wound Debridement. "Archives of Dermatology" doi:10.1001/archdermatol.2011.1895. [1]
  3. ^ Whitaker IS, Twine C, Whitaker MJ, Welck M, Brown CS, Shandall A (2007). "Larval therapy from antiquity to the present day: mechanisms of action, clinical applications and future potential". Postgraduate Medical Journal 83 (980): 409–13. doi:10.1136/pgmj.2006.055905. PMC 2600045. PMID 17551073. 
  4. ^ Sherman RA, Hall MJ, Thomas S (2000). "Medicinal maggots: an ancient remedy for some contemporary afflictions". Annu. Rev. Entomol. 45: 55–81. doi:10.1146/annurev.ento.45.1.55. PMID 10761570. 
  5. ^ Donnelly Jean (1998). "Wound Healing - from poultices to maggots. (A short synopsis of wound healing throughout the ages)". The Ulster Medical Journal 67 (Suppl 1): 47–51. PMC 2448900. PMID 9807955. 
  6. ^ Baer, W. S. (1931): The treatment of chronic osteomyelitis with the maggot (larvae of the blowfly). Journal of Bone and Joint Surgery 13: 438–475.
  7. ^ Hewitt, F. (1932): Osteomyelitis; Development of the use of maggots in treatment. American Journal of Nursing 32: 31-38.
  8. ^ McKeever, D. C. (1933): Maggots in treatment of osteomyelitis: A simple inexpensive method. Journal of Bone and Joint Surgery 15: 85–93.
  9. ^ Bethune, N. (1935): A case of chronic thoracic empyema treated with maggots. Can Med Assoc J 32: :301–302. (Cited at Norman Bethune and Edward Archibald: sung and unsung heroes)
  10. ^ http://web.archive.org/web/20070928212353/www.zoobiotic.org/maggots-in-wartime/
  11. ^ Sherman RA, Wyle F, Vulpe M (1995). "Maggot therapy for treating pressure ulcers in spinal cord injury patients". J Spinal Cord Med 18 (2): 71–4. PMID 7640976. 
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  14. ^ Nic Fleming, Maggots used to counter MRSA superbug, Telegraph.co.uk, March 5, 2007. available online
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  16. ^ FDA CDRH 510(k) summary LINK
  17. ^ The Pink Sheets Panel To Recommend Classifications For Five Preamendments Medical Devices Aug. 25–26 LINK
  18. ^ http://www.amazon.com/Honey-Maggots-Other-Medical-Marvels/dp/0395924928
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  20. ^ Monarch Labs package insert for Medical MaggotsTM http://www.monarchlabs.com/maggotpi.pdf
  21. ^ 186. Armstrong DG, Salas P, Short B, Martin BR, Kimbriel HR, Nixon BP, Boulton AJM, “Maggot Therapy in ‘Lower Extremity Hospice’ Wound Care: Fewer Complications and More Antibiotic-Free Days”, J Amer Podiatric Medical Association, 95:254-257, 2005
  22. ^ Bradley M, Cullum N, Sheldon T The debridement of chronic wounds: a systematic review National Institute for Health research Health Technol Assess. 1999;3(17 Pt 1):iii–iv, 1–78. http://www.ncbi.nlm.nih.gov/pubmed/10492854
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  28. ^ http://mic.sgmjournals.org/cgi/content/abstract/156/2/400
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  31. ^ V. Scavée, Fr.-X. Polis, J.-Cl. Schoevaerdts. "Maggot Therapy : Many Hands Make Light Work". Archived from the original on September 29, 2007. Retrieved 2007-05-06. 
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  33. ^ "Maggot therapy hope 'premature'". BBC News. 2009-03-20. Retrieved 2010-05-12. 
  34. ^ Sherman R. A., Hall M. J. R., Thomas S. (2000). "Medicinal Maggots: an Ancient Remedy for Some Contemporary Afflictions". Annu. Rev. Entomol 45: 55–81. 

External links[edit]