Holley attended the prestigious Cass Technical High School. Holley played violin and tuba when young and started playing bass while serving in the Navy. In the latter half of the 1940s he played with Dexter Gordon, Charlie Parker, and Ella Fitzgerald; in 1950 he and Oscar Peterson recorded duets, and he also played with Peterson and Charlie Smith as a trio. He was married to Minnie (Millicent) Walton née Aitcheson .
In the mid-1950s he moved to England and worked at the BBC. Upon his return to America he toured with Woody Herman in 1958 and with Al Cohn/Zoot Sims in 1959-60. A prolific studio musician, he played with Duke Ellington in 1964 and with the Kenny Burrell Trio, Coleman Hawkins, Lee Konitz, Roy Eldridge, Michel Legrand, Milt Buckner, Jay McShann and Quincy Jones in the 1960s and 1970s. From 1967 to 1970 he taught at the Berklee College of Music.
Holley was noted for singing along with his arco (bowed) bass solos, a technique Slam Stewart also used. Holley and Stewart recorded together on two albums in the 1970s.
- As sideman
- Kenny Burrell - Bluesin' Around (Columbia, 1962 ), Bluesy Burrell (Moodsville, 1962), Midnight Blue (1963)
- Coleman Hawkins - Good Old Broadway (Moodsville, 1962), The Jazz Version of No Strings (Moodsville, 1962), Coleman Hawkins Plays Make Someone Happy from Do Re Mi (Moodsville, 1962), Desafinado (Impulse!, 1962)
- Stanley Turrentine - Never Let Me Go (1963)
- Milt Jackson - For Someone I Love (Riverside, 1963)
- Quincy Jones - Quincy Jones Explores the Music of Henry Mancini (1964)
- Roland Kirk - Here Comes the Whistleman (1965)
- Frank Sinatra - L.A. Is My Lady (1984)
- Shirley Scott - The Soul Is Willing (Prestige, 1963), Drag 'em Out (Prestige, 1963)
- Jaki Byard - Family Man (Muse, 1978)
- Scott Yanow, Major Holley at Allmusic. Retrieved 2 May 2013.
- "Mule Holley, Bassist, Dead at 66; A Favorite Among Jazz Musicians", The New York Times