Mamavirus is one of the most complex viruses discovered. It is a virus belonging to the family mimiviridae, which includes mimivirus and mamavirus. The mimiviridae also belong to the family of the nucleocytoplasmic large DNA viruses (NCLDVs), which includes the Poxviridae, the Iridoviridae, the Asfarviridae, and the Phycodnaviridae Like mimivirus, mamavirus is very large, larger than many bacteria. Mamavirus is actually larger than mimivirus; thus, the name mamavirus, even though mimivirus encodes more than one thousand proteins in its genome of 1.2Mb.
Mamavirus was first reported in September 2008. Like mimivirus, mamavirus was isolated from an amoeba in a cooling tower. The mimiviridae were not discovered until recently because of their size; when filtered the mimiviridae stay with the bacteria which led scientists to believe they were also bacteria. Mimivirus was first isolated in 1992 when scientists were looking for the cause of a pneumonia outbreak in Bradford UK. Due to its size it was named “Bradford coccus” and put in a freezer with scientists thinking it was a bacterium. A decade later, Claverie and Didier Raoult discovered “Bradford coccus” was no bacterium when they tried to digest the cell wall with no success. Deciding to take a different route they looked at it under an electron microscope. They were surprised to find that it looked like a giant iridovirus, which are icosahedral viruses that infect insects, fish, and frogs. Knowing this paved the way for the discovery of mamavirus because scientists knew to look for other giant viruses.
Structure and Genome
Mamavirus, like other mimiviridae, is icosahedral with a core capsid and a peripheral fiber layer. It contains a linear double-stranded DNA genome which has a very high coding density that is characteristic of NCLDVs. The mimiviridae contain very similar genomes due to gene duplications, and a fair piece of the genome is associated with functions not previously found in a virus.
Mamavirus possesses its own transcription machinery, and it packages transcription proteins in its particles. Transcription is believed to occur in the core particles. The core releases viral DNA and forms a cytoplasmic replication factory where DNA replication begins and transcription of late genes occurs. The replication factory forms around the viral core and expands until it occupies a large fraction of the amoeba cell volume. Later stages of the replication cycle involve partially assembled procapsids undergoing DNA packaging.
While the mimiviridae were a surprise themselves, mamavirus contained an even bigger surprise. While looking at mamavirus under the electron microscope, Raoult discovered a second, small virus closely associated with mamavirus which was named Sputnik virophage, a satellite virus. Sputnik contains 21 genes and is very tiny compared to mamavirus; however, it is quite powerful in its effects on mamavirus. Sputnik cannot replicate in acanthamoeba cells without a simultaneous infection by mamavirus (or mimivirus) so it infects the viral factory mamavirus creates and hijacks it to replicate its own genome. This causes mamavirus to produce fewer viruses that are often deformed and less effective; there is also evidence of a partial thickening of the capsid. The fact that Sputnik can do this suggests that it is a viral parasite, and thus, was named the first virophage. A virophage is like bacteriophage viruses, which infects and sickens bacteria, but virophages infect viruses. Sputnik contains a circular double-stranded DNA of 18,343 base pairs, and is icosahedral in shape. Of the 21 genes it contains, eight encode proteins that have homologues. Of these eight, three are thought to be derived from mamavirus or mimivirus. This indicates that Sputnik can participate in gene-transfer processes and mediate lateral gene transfer between giant viruses.
Mamavirus has caused scientists to review the criteria of life; to start questioning if viruses are alive, revive the debate about the origin of DNA viruses and their possible role in the emergence of the eukaryotic nucleus.
- Van Etten, James L.; Leslie C. Lane, David D. Dunigan (13). "DNA Viruses: The Really Big Ones (Giruses)". Annual Review of Microbiology (Annual Reviewof Microbiology) 64: 83–99. doi:10.1146/annurev.micro.112408.134338. PMC 2936810. PMID 20690825.
- Claverie, Jean-Michel; Chantal Abergel (2009). "Mimivirus and its Virophage". Annual Review of Genetics. pp. 49–66.
- Ehrenberg, Rachel (10). "Enter the Virosphere: As evidence of the influence of viruses escalates, appreciation of these master manipulators grows". Science News (Science News) 176 (8): 22–25. doi:10.1002/scin.5591760820.
- Colson P, Yutin N, Shabalina SA, et al. (June 2011). "Viruses with more than 1000 genes: Mamavirus, a new Acanthamoeba castellanii mimivirus strain, and reannotation of mimivirus genes". Genome Biol Evol 3: 737–42. doi:10.1093/gbe/evr048. PMC 3163472. PMID 21705471.
- Pearson, Helen (7). "'Virophage' suggests viruses are alive". Nature. p. 677.
- La Scola, Bernard; Christelle Desnues, Isabelle Pagnier, Catherine Robert, Lina Barrassi, Ghislain Fournous, Michele Merchat, Marie Suzan-Monti, Patrick Forterre, Eugene Koonin, Didier Raoult (4). "The virophage as a unique parasite of the giant mimivirus". Nature. pp. 100–105.
- Desnues, C; D. Raoult (15). "Inside the Lifestyle of the Virophage". Intervirology. pp. 293–303.
- Sun, Siyang; Bernard La Scola, Valorie D. Bownam, Christopher M. Ryan, Julian P. Whitelegge, Didier Raoult, Michael G. Rossmann (1 2010). "Structural Studies of the Sputnik Virophage". Journal of Virology (Journal of Virology) 84 (2): 894–897. doi:10.1128/JVI.01957-09. PMC 2798384. PMID 19889775.
- Smallridge, Rachel (2008). "Virology: A Virus gets a Virus". Nature Reviews.