The locomotive was number 9903 and named Injun Joe. The following three cars received other names of Mark Twain characters, such as Becky Thatcher, Tom Sawyer and Huck Finn. The train was christened on October 25, 1935 in Hannibal, Missouri by a granddaughter of its namesake. The ceremony was broadcast coast to coast on CBS radio. Two days later, it entered revenue service.
It was retired by the Chicago, Burlington and Quincy Railroad (CB&Q) and then was purchased by Frank Dashner of Glenwood, Iowa in June 1960. In June 1962, the train was acquired by Ernie Hays for $6,500 and placed on exhibit at the Midwest Old Thresher Association grounds in Mount Pleasant, Iowa. In 1972, after many years of neglect, the train was sold to Alex Barkett of the Building Leasing Corporation, and moved to Kansas City.
After Mr. Barkett died, title transferred to the Civic Plaza National Bank and in September 1987 the train was moved to the Mid America Car Corporation. In 1984, Dave Stimson a player for the Kansas City Chiefs purchased the equipment. In 1987, the train was sold to John C. Lowe. He and two other individuals have formed the Mark Twain Corporation. Through 2007, the train was in unrestored condition and located in Minooka, Illinois.
On January 24, 2008, it was supposedly moved to Granite City, Illinois for display in a downtown redevelopment effort but was shown by Gateway Rail Services in nearby Madison, Illinois to a group from the 2008 St. Louis Railroad Prototype Modelers Meet in August, 2008. A 2011 report from a Burlington railfan says the train is still at Gateway.