Martyrs Bus Service is a bus and coach operator in Melbourne, Australia. As a Melbourne bus company, it operates one bus route under contract to the Government of Victoria.
Martyrs Bus Service was formed in July 1927 when Harry Martyr commenced a service from Warburton to Melbourne City Centre. In 1979 the business was sold to Hornibrook Bus Lines proprietor Geoff Mountjoy.
In September 1990 the Chirnside Park Shopping Centre to Melbourne City Centre portion of route 683 ceased. In November 2011 Martyrs Bus Service was sold to the Dineen Group.
As at May 2014 the fleet consisted of 54 buses and coaches. Fleet livery is white with light and dark blue stripes.