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|Maud Leonora Menten|
|Born||20 March 1879|
|Died||July 26, 1960
Leamington, Ontario, Canada
|Thesis||The Alkalinity of the Blood in Malignancy and Other Pathological Conditions; Together with Observations on the Relation of the Alkalinity of the Blood to Barometric Pressure (1916)|
|Known for||Michaelis-Menten equation, contributions to enzyme kinetics and histochemistry|
Maud Leonora Menten[pronunciation?] (March 20, 1879 – July 26, 1960) was a Canadian physician-scientist who made significant contributions to enzyme kinetics and histochemistry. Her name is associated with the famous Michaelis–Menten equation in biochemistry.
Maud Menten was born in Port Lambton, Ontario and studied medicine at the University of Toronto (B.A. 1904, M.B. 1907, M.D. 1911). She was among the first women in Canada to earn a medical doctorate. She completed her thesis work at University of Chicago. At that time women were not allowed to do research in Canada, so she decided to do research in other countries such as the United States and Germany.
In 1912 she moved to Berlin where she worked with Leonor Michaelis and co-authored their paper in Biochemische Zeitschrift (1913;49:333–369) which showed that the rate of an enzyme-catalyzed reaction is proportional to the amount of the enzyme-substrate complex. This relationship between reaction rate and enzyme-substrate concentration is known as the Michaelis-Menten equation. After studying with Michaelis in Germany she entered graduate school at the University of Chicago where she obtained her PhD in 1916. Her dissertation was titled "The Alkalinity of the Blood in Malignancy and Other Pathological Conditions; Together with Observations on the Relation of the Alkalinity of the Blood to Barometric Pressure". Menten worked at the University of Pittsburgh (1923–1950), becoming a Professor in the School of Medicine and head of pathology at the Children's Hospital of Pittsburgh. Her final academic post was as a research fellow at the British Columbia Medical Research Institute (1951–1953).
Little is known about her parents and childhood other than that the Menten family moved to Harrison Mills, where Maud's mother worked as a postmistress. After completing secondary school, Menten attended the University of Toronto where she earned a bachelor of arts degree in 1904 and a master's degree in physiology in 1907. While earning her graduate degree, she worked as a demonstrator in the university's physiology lab.
A talented student, Menten was appointed a fellow at the Rockefeller Institute for Medical Research in New York City in 1907. There, she studied the effect of radium bromide on cancerous tumors in rats. Menten and two other scientists published the results of their experiment, producing the institute's first monograph. After a year at the Institute, Menten worked as an intern at the New York Infirmary for Women and Children. She returned to Canada and began studies at the University of Toronto a year later. In 1911 she became one of the first Canadian women to receive a doctor of medicine degree.
Her most famous work was on enzyme kinetics together with Michaelis, based on earlier findings of Victor Henri. This resulted in the Michaelis–Menten equations. Menten also invented the azo-dye coupling reaction for alkaline phosphatase, which is still used in histochemistry. She characterised bacterial toxins from B. paratyphosus, Streptococcus scarlatina and Salmonella ssp. and conducted the first electrophoretic separation of proteins in 1944. She worked on the properties of hemoglobin, regulation of blood sugar level, and kidney function. She wrote or co-wrote about 100 research papers.
Personal Life and Aside Work
Despite suffering from arthritis she was also an accomplished musician and painter; there were several exhibitions of her paintings.
Skloot portrays Menten as a petite dynamo of a woman who wore "Paris hats, blue dresses with stained-glass hues, and Buster Brown shoes." She drove a Model T Ford through the University of Pittsburgh area for some 32 years and enjoyed many adventurous and artistic hobbies. She played the clarinet, painted paintings worthy of art exhibitions, climbed mountains, went on an Arctic expedition, and enjoyed astronomy. She also mastered several languages, including Russian, French, German, Italian, and at least one Native-American language. Although Menten did most of her research in the United States, she retained her Canadian citizenship throughout her life. After her retirement from the University of Pittsburgh in 1950, she returned to Canada where she continued to do cancer research at the British Columbia Medical Research Institute. Poor health forced Menten's retirement in 1955, and she died July 20, 1960, at the age of 81, in Leamington, Ontario.
Throughout her career Menten was affiliated with many scientific societies, and in 1998 she was posthumously inducted into the Canadian Medical Hall of Fame. She was also honored at the University of Toronto with a plaque and at the University of Pittsburgh with memorial lectures and a named chair.
At Menten's death, colleagues Aaron H. Stock and Anna-Mary Carpenter honored the Canadian biochemist in an obituary in Nature: "Menten was untiring in her efforts on behalf of sick children. She was an inspiring teacher who stimulated medical students, resident physicians and research associates to their best efforts. She will long be remembered by her associates for her keen mind, for a certain dignity of manner, for unobtrusive modesty, for her wit, and above all for her enthusiasm for research."
- Menten ML., Willms, M., Wright, WD. "Nucleic acid content of splenic lymphocytes in normal and leukemic mice." Cancer Research, Vol 13 (1953) pp. 729–732
- Neale, AE., Menten, ML. "Tumors of the thymus in children." American Journal of Diseases of Children, Vol 76 (1948) pp. 102–108
- Menten, ML., Fetterman, GH. "Coronary sclerosis in infancy - report of 3 autopsied cases, 2 in siblings." American Journal of Clinical Pathology, Vol 18 (1948) pp. 805–810
- Menten, ML., Janouch, M. "Changes in alkaline phosphatase of kidney following renal damage with alloxan." Proceedings of the Society for Experimental Biology and Medicine, Vol 63 (1946) pp. 33–37
- Troll, MM., Menten, ML. "Salicylate poisoning - report of 4 cases." American Journal of Diseases of Children, Vol 69 (1945) pp. 37–43
- Menten, ML., Junge, J., Green, MH. "Distribution of alkaline phosphatase in kidney following the use of histochemical azo dye test." Proceedings of the Society for Experimental Biology and Medicine, Vol 57 (1944) pp. 82–86
- Menten, ML., Junge, J., Green, MH. "A coupling histochemical azo dye test for alkaline phosphatase in the kidney." Journal of Biological Chemistry, Vol 153 (1944) pp 471–477
- Andersch, MA,. Wilson, DA,. Menten, ML. "Sedimentation constants and electrophoretic mobilities of adult and fetal carbonylhemoglobin." Journal of biological Chemistry, Vol 153 (1944) pp. 301–305
- King, CG., Menten, ML. "The influence of vitamin C level upon resistance to diphtheria toxin I. Changes in body weight and duration of life." Journal of Nutrition, Vol 10 (1935) pp. 129–140
- Menten, ML. "Changes in the blood sugar of the cod, sculpin, and pollock during asphyxia." Journal of Biological Chemistry, Vol 72 (1927) pp. 249–253
- Menten, ML., "Pathological lesions produced in the kidney by small doses of mercuric chloride." Journal of Medical Research, Vol 43 (1922) pp. 315–321
- Menten, ML., "A study of the oxidase reaction with a-naphthol and paraphenylenediamine." Journal of Medical Research, Vol 40 (1919) pp. 433 - U22
- Michaelis L., Menten, ML. "The kinetics of invertin action" Biochemische Zeitschrift, Vol 49 (1913) pp. 335–369 (Translation by T.R.C. Boyde in FEBS Letters, vol 587 (2013) pp. 2712–2720)
- Michaelis, Leonor (4 Feb 1913). "Die Kinetik der Invertinwirkung" [The kinetics of invertin action]. Biochemische Zeitschrift 49: 335–369. doi:10.1016/j.febslet.2013.07.015.
- Menten, M. (1919). "A Study of the Oxidase Reaction with alpha-Naphthol and Paraphenylenediamine". The Journal of medical research 40 (3): 433–458.3. PMC 2104435. PMID 19972493.
- Skloot, Rebecca (Oct 2000). "Some called her MIss Menten". University of Pittsburgh School of Medicine magazine. Retrieved 14 October 2014.
- Menten, M.; McCloskey, G. (1951). "Histopathology and Etiology of Pneumonia in Children Dying after Antibacterial Therapy". The American journal of pathology 27 (3): 477–491. PMC 1937251. PMID 19970982.
- JCE Online: Biographical Snapshots: Snapshot
- "Dr. Maud Menten". The Canadian Medical Hall of Fame. Retrieved 14 October 2014.
- Canadian Medical Hall of Fame – brief biography with photo and video
- Some called her Miss Menten by Rebecca Skloot in Pittmed (University of Pittsburgh School of Medicine magazine), October 2000
- Kenneth A. Johnson and Roger S. Goody "The Original Michaelis Constant: Translation of the 1913 Michaelis-Menten Paper" Biochemistry. 2011 October 4; 50(39):8264–8269. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3381512/