Pertile grew up in Indiana and Illinois. She attended and graduated from Hammond High School, Hammond, Indiana in 1959. Following graduation, she moved to Los Angeles, where she became a contract player with Universal Pictures and had guest spots on several TV shows.
During the second season of the Playboy's Penthouse TV series (1960–61), Pertile became a regular cast member. It was there that she first met Hugh Hefner.
According to The Playmate Book, Pertile died on November 28, 1997. The cause of death was complications following heart surgery.