|Methyl violet 2B|
|Appearance||Green to dark-green powder|
137 °C (279 °F) – decomposes
|Solubility in water||Soluble in water, ethanol, insoluble in xylene|
| (what is: / ?)
Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa)
Methyl violet is a family of organic compounds that are mainly used as dyes. Depending on the amount of attached methyl groups, the color of the dye can be altered. Its main use is as a purple dye for textiles and to give deep violet colors in paint and ink. Methyl violet 10B is also known as crystal violet (and many other names) and has medical uses.
The term methyl violet encompasses three compounds that differ in the number of methyl groups attached to the amine functional group. They are all soluble in water, ethanol, diethylene glycol and dipropylene glycol.
|Name||Methyl violet 2B||Methyl violet 10B (Crystal violet)|
|ChemSpider ID||2006225||3349, 9080056, 10354393|
Methyl violet 2B
Methyl violet 2B (IUPAC name:N-(4-(bis(4-(dimethylamino)phenyl)methylene)cyclohexa-2,5-dien-1-ylidene)methanaminium chloride) is a green powder which is soluble in water in ethanol and water, but not in xylene. It appears yellow in solution of low pH (~0.15) and changes to violet with pH increasing toward 3.2.
Methyl violet 10B
Methyl violet 10B has six methyl groups. It is known in medicine as Gentian violet (or crystal violet or pyoctanin(e)) and is the active ingredient in a Gram stain, used to classify bacteria. It is used as a pH indicator, with a range between 0 and 1.6. The protonated form (found in acidic conditions) is yellow, turning blue-violet above pH levels of 1.6. Gentian violet destroys cells and can be used as a disinfectant. Compounds related to methyl violet are potential carcinogens.
Methyl violet 10B also inhibits the growth of many Gram positive bacteria, except streptococci. When used in conjunction with nalidixic acid (which destroys gram-negative bacteria), it can be used to isolate the streptococci bacteria for the diagnosis of an infection.
Methyl violet 10B also binds to DNA. This means it can be used in cell viability assays in biochemistry. However, this binding to DNA will cause replication errors in living tissue, possibly leading to mutations and cancer.
Methyl violet is a mutagen and mitotic poison, therefore concerns exist regarding the ecological impact of the release of methyl violet into the environment. Methyl violet has been used in vast quantities for textile and paper dyeing, and 15% of such dyes produced worldwide are released to environment in wastewater. Numerous methods have been developed to treat methyl violet pollution. The three most prominent are chemical bleaching, biodegradation, and photodegradation.
Chemical bleaching is achieved by oxidation or reduction. Oxidation can destroy the dye completely, e.g. through the use of sodium hypochlorite (NaClO, common bleach) or hydrogen peroxide. Reduction of methyl violet occurs in microorganisms but can be attained chemically using sodium dithionite.
Biodegradation has been well investigated because of its relevance to sewage plants with specialized microorganisms. Two microorganisms that have been studied in depth are the white rot fungus and the bacterium Nocardia Corallina.
Many others methods have been developed to treat the contamination of dyes in a solution, including electrochemical degradation, ion exchange, laser degradation, and absorption onto various solids such as activated charcoal.
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