Yakushin played football for Moscow clubs STS (1928–1929), SKiG (1931–1933), and Dynamo (1933–1944). He scored one goal in his three international matches for the Soviet national team. In the 1930s he also played field hockey for Dynamo, favoring hockey to football.
As a manager he coached Dynamo Moscow from 1944 to 1950 and from 1953 to 1960, winning six Soviet titles (1945, 1949, 1954, 1955, 1957, 1959). He was the head coach of the USSR national football team in 1959 and from 1967 to 1968.