Mitochondrial DNA (mtDNA or mDNA) is the DNA located in mitochondria, cellular organelles within eukaryotic cells that convert chemical energy from food into a form that cells can use, adenosine triphosphate (ATP). Mitochondrial DNA is only a small portion of the DNA in a eukaryotic cell; most of the DNA can be found in the cell nucleus and, in plants, in the chloroplast.
In humans, mitochondrial DNA can be assessed as the smallest chromosome coding for 37 genes and containing approximately 16,600 base pairs. Human mitochondrial DNA was the first significant part of the human genome to be sequenced. In most species, including humans, mtDNA is inherited solely from the mother.
The DNA sequence of mtDNA has been determined from a large number of organisms and individuals (including some organisms that are extinct), and the comparison of those DNA sequences represents a mainstay of phylogenetics, in that it allows biologists to elucidate the evolutionary relationships among species. It also permits an examination of the relatedness of populations, and so has become important in anthropology and field biology[clarification needed].
- 1 Origin
- 2 Mitochondrial inheritance
- 3 Structure
- 4 Replication
- 5 Mutations
- 6 Use in identification
- 7 History
- 8 Mitochondrial sequence databases
- 9 See also
- 10 References
Nuclear and mitochondrial DNA are thought to be of separate evolutionary origin, with the mtDNA being derived from the circular genomes of the bacteria that were engulfed by the early ancestors of today's eukaryotic cells. This theory is called the endosymbiotic theory. Each mitochondrion is estimated to contain 2–10 mtDNA copies. In the cells of extant organisms, the vast majority of the proteins present in the mitochondria (numbering approximately 1500 different types in mammals) are coded for by nuclear DNA, but the genes for some of them, if not most, are thought to have originally been of bacterial origin, having since been transferred to the eukaryotic nucleus during evolution.
In most multicellular organisms, mtDNA is inherited from the mother (maternally inherited). Mechanisms for this include simple dilution (an egg contains on average 200,000 mtDNA molecules, whereas a healthy human sperm was reported to contain on average 5 molecules ), degradation of sperm mtDNA in the male genital tract, in the fertilized egg, and, at least in a few organisms, failure of sperm mtDNA to enter the egg. Whatever the mechanism, this single parent (uniparental inheritance) pattern of mtDNA inheritance is found in most animals, most plants and in fungi as well.
In sexual reproduction, mitochondria are normally inherited exclusively from the mother; the mitochondria in mammalian sperm are usually destroyed by the egg cell after fertilization. Also, most mitochondria are present at the base of the sperm's tail, which is used for propelling the sperm cells; sometimes the tail is lost during fertilization. In 1999 it was reported that paternal sperm mitochondria (containing mtDNA) are marked with ubiquitin to select them for later destruction inside the embryo. Some in vitro fertilization techniques, particularly injecting a sperm into an oocyte, may interfere with this.
The fact that mitochondrial DNA is maternally inherited enables genealogical researchers to trace maternal lineage far back in time. (Y-chromosomal DNA, paternally inherited, is used in an analogous way to determine the patrilineal history.) This is accomplished on human mitochondrial DNA by sequencing one or more of the hypervariable control regions (HVR1 or HVR2) of the mitochondrial DNA, as with a genealogical DNA test. HVR1 consists of about 440 base pairs. These 440 base pairs are then compared to the control regions of other individuals (either specific people or subjects in a database) to determine maternal lineage. Most often, the comparison is made to the revised Cambridge Reference Sequence. Vilà et al. have published studies tracing the matrilineal descent of domestic dogs to wolves. The concept of the Mitochondrial Eve is based on the same type of analysis, attempting to discover the origin of humanity by tracking the lineage back in time.
mtDNA is highly conserved, and its relatively slow mutation rates (compared to other DNA regions such as microsatelites) make it useful for studying the evolutionary relationships—phylogeny—of organisms. Biologists can determine and then compare mtDNA sequences among different species and use the comparisons to build an evolutionary tree for the species examined. However, due the slow mutation rates it experiences, it is often hard to distinguish between closely related species to any large degree, so other methods of analysis must be used.
Doubly uniparental inheritance of mtDNA is observed in bivalve mollusks. In those species, females have only one type of mtDNA (F), whereas males have F type mtDNA in their somatic cells, but M type of mtDNA (which can be as much as 30% divergent) in germline cells. Paternally inherited mitochondria have additionally been reported in some insects such as fruit flies, honeybees, and periodical cicadas.
Evidence supports rare instances of male mitochondrial inheritance in some mammals as well. Specifically, documented occurrences exist for mice, where the male-inherited mitochondria was subsequently rejected. It has also been found in sheep, and in cloned cattle. It has been found in a single case in a human male.
Although many of these cases involve cloned embryos or subsequent rejection of the paternal mitochondria, others document in vivo inheritance and persistence under lab conditions.
An artificial reproductive process known as Three Parent In Vitro Fertilization (TPIVF) results in offspring containing mtDNA from a donor female, and nuclear DNA from another female and a male. In the process, the nucleus of an egg is inserted into the cytoplasm of an egg from a donor female which has had its nucleus removed, but still contains the donor female's mtDNA. The composite egg is then fertilized with the male's sperm. The procedure is used when a woman with genetically defective mitochondria wishes to procreate and produce offspring with healthy mitochondria.
In most multicellular organisms, the mtDNA is organized as a circular, covalently closed, double-stranded DNA. But in many unicellular (e.g. the ciliate Tetrahymena or the green alga Chlamydomonas reinhardtii) and in rare cases also in multicellular organisms (e.g. in some species of Cnidaria) the mtDNA is found as linearly organized DNA. Most of these linear mtDNAs possess telomerase independent telomeres (i.e. the ends of the linear DNA) with different modes of replication, which have made them interesting objects of research, as many of these unicellular organisms with linear mtDNA are known pathogens.
For human mitochondrial DNA (and probably for that of metazoans in general), 100-10,000 separate copies of mtDNA are usually present per cell (egg and sperm cells are exceptions). In mammals, each double-stranded circular mtDNA molecule consists of 15,000-17,000 base pairs. The two strands of mtDNA are differentiated by their nucleotide content, with a guanine-rich strand referred to as the heavy strand (or H-strand) and a cytosine-rich strand referred to as the light strand (or L-strand). The heavy strand encodes 28 genes, and the light strand encodes 9 genes for a total of 37 genes. Of the 37 genes, 13 are for proteins (polypeptides), 22 are for transfer RNA (tRNA) and two are for the small and large subunits of ribosomal RNA (rRNA). This pattern is also seen among most metazoans, although in some cases one or more of the 37 genes is absent and the mtDNA size range is greater. Even greater variation in mtDNA gene content and size exists among fungi and plants, although there appears to be a core subset of genes that are present in all eukaryotes (except for the few that have no mitochondria at all). Some plant species have enormous mtDNAs (as many as 2,500,000 base pairs per mtDNA molecule) but, surprisingly, even those huge mtDNAs contain the same number and kinds of genes as related plants with much smaller mtDNAs.
As far as transcription concerns, at least in animals, each strand is transcribed continuously and produces a polycistronic RNA molecule. Mitochondrial genes for ATP8 and ATP6 as well as ND4L and ND4 overlap. Between most (but not all) protein-coding regions, tRNAs are present. During transcription, the tRNAs acquire their characteristic L-shape that gets recognized and cleaved by specific enzymes. Mutations in mitochondrial tRNAs can be responsible for severe diseases like the MELAS and MERRF syndromes.
The genome of the mitochondrion of the cucumber (Cucumis sativus) consists of three circular chromosomes (lengths 1556, 84 and 45 kilobases), which are entirely or largely autonomous with regard to their replication.
Mitochondrial DNA is replicated by the DNA polymerase gamma complex which is composed of a 140 kDa catalytic DNA polymerase encoded by the POLG gene and two 55 kDa accessory subunits encoded by the POLG2 gene. The replisome machinery is formed by DNA polymerase, TWINKLE and mitochondrial SSB proteins. TWINKLE is a helicase, which unwinds short stretches of dsDNA in the 5′ to 3′ direction.
During embryogenesis, replication of mtDNA is strictly down-regulated from the fertilized oocyte through the preimplantation embryo. At the blastocyst stage, the onset of mtDNA replication is specific to the cells of the trophectoderm. In contrast, the cells of the inner cell mass restrict mtDNA replication until they receive the signals to differentiate to specific cell types.
The concept that mtDNA is particularly susceptible to reactive oxygen species generated by the respiratory chain due to its proximity remains controversial. mtDNA does not accumulate any more oxidative base damage than nuclear DNA. It has been reported that at least some types of oxidative DNA damage are repaired more efficiently in mitochondria than they are in the nucleus. mtDNA is packaged with proteins which appear to be as protective as proteins of the nuclear chromatin. Moreover, mitochondria evolved an unique mechanism which maintains mtDNA integrity through degradation of excessively damaged genomes followed by replication of intact/repaired mtDNA. This mechanism is not present in the nucleus and is enabled by multiple copies of mtDNA present in mitochondria  The outcome of mutation in mtDNA may be an alteration in the coding instructions for some proteins, which may have an effect on organism metabolism and/or fitness.
Mutations of mitochondrial DNA can lead to a number of illnesses including exercise intolerance and Kearns–Sayre syndrome (KSS), which causes a person to lose full function of heart, eye, and muscle movements. Some evidence suggests that they might be major contributors to the aging process and age-associated pathologies.
Use in disease diagnosis
Relationship with aging
Though the idea is controversial, some evidence suggests a link between aging and mitochondrial genome dysfunction. In essence, mutations in mtDNA upset a careful balance of reactive oxygen species (ROS) production and enzymatic ROS scavenging (by enzymes like superoxide dismutase, catalase, glutathione peroxidase and others). However, some mutations that increase ROS production (e.g., by reducing antioxidant defenses)in worms increase, rather than decrease, their longevity. Also, naked mole rats, rodents about the size of mice, live about eight times longer than mice despite having reduced, compared to mice, antioxidant defenses and increased oxidative damage to biomolecules. Once, there was thought to be a positive feedback loop at work (a 'Vicious Cycle'); as mitochondrial DNA accumulates genetic damage caused by free radicals, the mitochondria lose function and leak free radicals into the cytosol. A decrease in mitochondrial function reduces overall metabolic efficiency. However, this concept was conclusively disproved when it was demonstrated that mice, which were genetically altered to accumulate mtDNA mutations at accelerated rate do age prematurely, but their tissues do not produce more ROS as predicted by the 'Vicious Cycle' hypothesis. Supporting a link between longevity and mitochondrial DNA, some studies have found correlations between biochemical properties of the mitochondrial DNA and the longevity of species. Extensive research is being conducted to further investigate this link and methods to combat aging. Presently, gene therapy and nutraceutical supplementation are popular areas of ongoing research. Bjelakovic et al. analyzed the results of 78 studies between 1977 and 2012, involving a total of 296,707 participants, and concluded that antioxidant supplements do not reduce all-cause mortality nor extend lifespan, while some of them, such as beta carotene, vitamin E, and higher doses of vitamin A, may actually increase mortality.
Use in identification
Unlike nuclear DNA, which is inherited from both parents and in which genes are rearranged in the process of recombination, there is usually no change in mtDNA from parent to offspring. Although mtDNA also recombines, it does so with copies of itself within the same mitochondrion. Because of this and because the mutation rate of animal mtDNA is higher than that of nuclear DNA, mtDNA is a powerful tool for tracking ancestry through females (matrilineage) and has been used in this role to track the ancestry of many species back hundreds of generations.
The low effective population size and rapid mutation rate (in animals) makes mtDNA useful for assessing genetic relationships of individuals or groups within a species and also for identifying and quantifying the phylogeny (evolutionary relationships; see phylogenetics) among different species, provided they are not too distantly related. To do this, biologists determine and then compare the mtDNA sequences from different individuals or species. Data from the comparisons is used to construct a network of relationships among the sequences, which provides an estimate of the relationships among the individuals or species from which the mtDNAs were taken. This approach has limits that are imposed by the rate of mtDNA sequence change. In animals, the high mutation rate makes mtDNA most useful for comparisons of individuals within species and for comparisons of species that are closely or moderately-closely related, among which the number of sequence differences can be easily counted. As the species become more distantly related, the number of sequence differences becomes very large; changes begin to accumulate on changes until an accurate count becomes impossible.
Mitochondrial DNA was admitted into evidence for the first time ever in 1996 during State of Tennessee v. Paul Ware.
In the 1998 court case of Commonwealth of Pennsylvania v. Patricia Lynne Rorrer, mitochondrial DNA was admitted into evidence in the State of Pennsylvania for the first time. The case was featured in episode 55 of season 5 of the true crime drama series Forensic Files (season 5).
Mitochondrial DNA was first admitted into evidence in California in the successful prosecution of David Westerfield for the 2002 kidnapping and murder of 7-year-old Danielle van Dam in San Diego: it was used for both human and dog identification. This was the first trial in the U.S. to admit canine DNA.
Mitochondrial DNA was discovered in the 1960s by Margit M. K. Nass and Sylvan Nass by electron microscopy as DNase-sensitive threads inside mitochondria, and by Ellen Haslbrunner, Hans Tuppy and Gottfried Schatz by biochemical assays on highly purified mitochondrial fractions.
Mitochondrial sequence databases
Several specialized databases have been founded to collect mitochondrial genome sequences and other information. Although most of them focus on sequence data, some of them include phylogenetic or functional information.
- MitoSatPlant: Mitochondrial microsatellites database of viridiplantae.
- MitoBreak: the mitochondrial DNA breakpoints database.
- MitoFish and MitoAnnotator: a mitochondrial genome database of fish. See also Cawthorn et al.
- MitoZoa 2.0: a database for comparative and evolutionary analyses of mitochondrial genomes in Metazoa.
- InterMitoBase: an annotated database and analysis platform of protein-protein interactions for human mitochondria.
- Mitome: a database for comparative mitochondrial genomics in metazoan animals (no longer available)
- MitoRes: a resource of nuclear-encoded mitochondrial genes and their products in metazoa (apparently no longer being updated)
- Iborra, Francisco J; Kimura, Hiroshi; Cook, Peter R (2004). "The functional organization of mitochondrial genomes in human cells". BMC Biology 2: 9. doi:10.1186/1741-7007-2-9. PMC 425603. PMID 15157274.
- Sykes, B (10 September 2003). "Mitochondrial DNA and human history". The Human Genome. Wellcome Trust. Retrieved 5 February 2012.
- "Mitochondrial DNA: The Eve Gene". Bradshaw Foundation. Bradshaw Foundation. Retrieved 5 November 2012.
- Wiesner, Rudolf J.; Rüegg, J.Caspar; Morano, Ingo (1992). "Counting target molecules by exponential polymerase chain reaction: Copy number of mitochondrial DNA in rat tissues". Biochemical and Biophysical Research Communications 183 (2): 553–9. doi:10.1016/0006-291X(92)90517-O. PMID 1550563.
- Gabriel, Maria San; Chan, Sam W.; Alhathal, Naif; Chen, Junjian Z.; Zini, Armand (2012). "Influence of microsurgical varicocelectomy on human sperm mitochondrial DNA copy number: A pilot study". Journal of Assisted Reproduction and Genetics 29 (8): 759–64. doi:10.1007/s10815-012-9785-z. PMC 3430774. PMID 22562241.
- Schatten, Gerald; Sutovsky, Peter; Moreno, Ricardo D.; Ramalho-Santos, João; Dominko, Tanja; Simerly, Calvin (1999). "Development: Ubiquitin tag for sperm mitochondria". Nature 402 (6760): 371–2. Bibcode:1999Natur.402..371S. doi:10.1038/46466. PMID 10586873. Discussed in: Travis, John (2000). "Mom's Eggs Execute Dad's Mitochondria". Science News 157: 5. doi:10.2307/4012086. JSTOR 4012086. Archived from the original on December 19, 2007.
- Vila, C.; Savolainen, P; Maldonado, J. E.; Amorim, I. R.; Rice, J. E.; Honeycutt, R. L.; Crandall, K. A.; Lundeberg, J; Wayne, R. K. (1997). "Multiple and Ancient Origins of the Domestic Dog". Science 276 (5319): 1687–9. doi:10.1126/science.276.5319.1687. PMID 9180076.
- Passamonti, Marco; Ghiselli, Fabrizio (2009). "Doubly Uniparental Inheritance: Two Mitochondrial Genomes, One Precious Model for Organelle DNA Inheritance and Evolution". DNA and Cell Biology 28 (2): 79–89. doi:10.1089/dna.2008.0807. PMID 19196051.
- Kondo R; Matsuura ET; Chigusa SI (1992). "Further observation of paternal transmission of Drosophila mitochondrial DNA by PCR selective amplification method". Genet. Res. 59 (2): 81–4. doi:10.1017/S0016672300030287. PMID 1628820.
- Meusel, Michael S.; Moritz, Robin F. A. (1993). "Transfer of paternal mitochondrial DNA during fertilization of honeybee (Apis mellifera L.) eggs". Current Genetics 24 (6): 539–43. doi:10.1007/BF00351719. PMID 8299176.
- Fontaine, Kathryn M.; Cooley, John R.; Simon, Chris (2007). "Evidence for Paternal Leakage in Hybrid Periodical Cicadas (Hemiptera: Magicicada spp.)". PLoS ONE 2 (9): e892. Bibcode:2007PLoSO...2..892F. doi:10.1371/journal.pone.0000892. PMC 1963320. PMID 17849021.
- Gyllensten, Ulf; Wharton, Dan; Josefsson, Agneta; Wilson, Allan C. (1991). "Paternal inheritance of mitochondrial DNA in mice". Nature 352 (6332): 255–7. Bibcode:1991Natur.352..255G. doi:10.1038/352255a0. PMID 1857422.
- Shitara H; Hayashi JI; Takahama S; Kaneda H; Yonekawa H (1998). "Maternal inheritance of mouse mtDNA in interspecific hybrids: segregation of the leaked paternal mtDNA followed by the prevention of subsequent paternal leakage". Genetics 148 (2): 851–7. PMC 1459812. PMID 9504930.
- Zhao, X; Li, N; Guo, W; Hu, X; Liu, Z; Gong, G; Wang, A; Feng, J; Wu, C (2004). "Further evidence for paternal inheritance of mitochondrial DNA in the sheep (Ovis aries)". Heredity 93 (4): 399–403. doi:10.1038/sj.hdy.6800516. PMID 15266295.
- Steinborn, Ralf; Zakhartchenko, Valeri; Jelyazkov, Jivko; Klein, Dieter; Wolf, Eckhard; Müller, Mathias; Brem, Gottfried (1998). "Composition of parental mitochondrial DNA in cloned bovine embryos". FEBS Letters 426 (3): 352–6. doi:10.1016/S0014-5793(98)00350-0. PMID 9600265.
- Schwartz, Marianne; Vissing, John (2002). "Paternal Inheritance of Mitochondrial DNA". New England Journal of Medicine 347 (8): 576–80. doi:10.1056/NEJMoa020350. PMID 12192017.
- Frith, Maxine (October 14, 2003). "The Independent".
- Nosek, Jozef; Tomáška, L'Ubomı́r; Fukuhara, Hiroshi; Suyama, Yoshitaka; Kováč, Ladislav (1998). "Linear mitochondrial genomes: 30 years down the line". Trends in Genetics 14 (5): 184–8. doi:10.1016/S0168-9525(98)01443-7. PMID 9613202.
- Balaresque, Patricia; Bowden, Georgina R.; Adams, Susan M.; Leung, Ho-Yee; King, Turi E.; Rosser, Zoë H.; Goodwin, Jane; Moisan, Jean-Paul; Richard, Christelle; Millward, Ann; Demaine, Andrew G.; Barbujani, Guido; Previderè, Carlo; Wilson, Ian J.; Tyler-Smith, Chris; Jobling, Mark A. (2010). "A Predominantly Neolithic Origin for European Paternal Lineages". PLoS Biology 8 (1): e1000285. doi:10.1371/journal.pbio.1000285. PMC 2799514. PMID 20087410.
- Ward, Bernard L.; Anderson, Robert S.; Bendich, Arnold J. (1981). "The mitochondrial genome is large and variable in a family of plants (Cucurbitaceae)". Cell 25 (3): 793–803. doi:10.1016/0092-8674(81)90187-2. PMID 6269758.
- Taylor, Robert W.; Turnbull, Doug M. (29 April 2015). "Mitochondrial DNA Mutations In Human Disease".
- Alverson, Andrew J; Rice, Danny W; Dickinson, Stephanie; Barry, Kerrie; Palmer, Jeffrey D (2011). "Origins and Recombination of the Bacterial-Sized Multichromosomal Mitochondrial Genome of Cucumber". The Plant Cell 23 (7): 2499–513. doi:10.1105/tpc.111.087189. JSTOR 41433488. PMC 3226218. PMID 21742987.
- Yakubovskaya, E.; Chen, Z.; Carrodeguas, J. A.; Kisker, C.; Bogenhagen, D. F. (2005). "Functional Human Mitochondrial DNA Polymerase Forms a Heterotrimer". Journal of Biological Chemistry 281 (1): 374–82. doi:10.1074/jbc.M509730200. PMID 16263719.
- Jemt, E.; Farge, G.; Backstrom, S.; Holmlund, T.; Gustafsson, C. M.; Falkenberg, M. (2011). "The mitochondrial DNA helicase TWINKLE can assemble on a closed circular template and support initiation of DNA synthesis". Nucleic Acids Research 39 (21): 9238–49. doi:10.1093/nar/gkr653. PMC 3241658. PMID 21840902.
- St. John, J. C.; Facucho-Oliveira, J.; Jiang, Y.; Kelly, R.; Salah, R. (2010). "Mitochondrial DNA transmission, replication and inheritance: A journey from the gamete through the embryo and into offspring and embryonic stem cells". Human Reproduction Update 16 (5): 488–509. doi:10.1093/humupd/dmq002. PMID 20231166.
- Alexeyev, Mikhail F. (2009). "Is there more to aging than mitochondrial DNA and reactive oxygen species?". FEBS Journal 276 (20): 5768–87. doi:10.1111/j.1742-4658.2009.07269.x. PMC 3097520. PMID 19796285.
- Anson, R. M.; Hudson, E; Bohr, V. A. (2000). "Mitochondrial endogenous oxidative damage has been overestimated". FASEB Journal 14 (2): 355–60. PMID 10657991.
- Thorslund, Tina; Sunesen, Morten; Bohr, Vilhelm A.; Stevnsner, Tinna (2002). "Repair of 8-oxoG is slower in endogenous nuclear genes than in mitochondrial DNA and is without strand bias". DNA Repair 1 (4): 261–73. doi:10.1016/S1568-7864(02)00003-4. PMID 12509245.
- Guliaeva, N. A.; Kuznetsova, E. A.; Gaziev, A. I. (2006). "Белки, ассоциированные с митохондриальной ДНК, защищают ее от воздействия рентгеновского излучения и перекиси водорода" [Proteins associated with mitochondrial DNA protect it against the action of X-rays and hydrogen peroxide]. Biofizika (in Russian) 51 (4): 692–7. PMID 16909848.
- Alexeyev, M.; Shokolenko, I.; Wilson, G.; Ledoux, S. (2013). "The Maintenance of Mitochondrial DNA Integrity--Critical Analysis and Update". Cold Spring Harbor Perspectives in Biology 5 (5): a012641. doi:10.1101/cshperspect.a012641. PMID 23637283.
- Hogan, C. Michael (2010). "Mutation". In Monosson, E.; Cleveland, C. J. Encyclopedia of Earth. Washington DC: National Council for Science and the Environment.
- Ledoux, Susan P.; Alexeyev, Mikhail F.; Wilson, Glenn L. (2004). "Mitochondrial DNA and aging". Clinical Science 107 (4): 355–64. doi:10.1042/CS20040148. PMID 15279618.
- Reguly, Brian; Jakupciak, John P.; Parr, Ryan L. (2010). "3.4 kb mitochondrial genome deletion serves as a surrogate predictive biomarker for prostate cancer in histopathologically benign biopsy cores". Canadian Urological Association Journal 4 (5): E118–22. PMC 2950771. PMID 20944788.
- Robinson, K; Creed, J; Reguly, B; Powell, C; Wittock, R; Klein, D; Maggrah, A; Klotz, L; Parr, R L; Dakubo, G D (2010). "Accurate prediction of repeat prostate biopsy outcomes by a mitochondrial DNA deletion assay". Prostate Cancer and Prostatic Diseases 13 (2): 126–31. doi:10.1038/pcan.2009.64. PMID 20084081.
- de Grey, Aubrey. The Mitochondrial Free Radical Theory of Aging (PDF). ISBN 1-57059-564-X.[page needed]
- Lewis, Kaitlyn N.; Andziak, Blazej; Yang, Ting; Buffenstein, Rochelle (2013). "The Naked Mole-Rat Response to Oxidative Stress: Just Deal with It". Antioxidants & Redox Signaling 19 (12): 1388–99. doi:10.1089/ars.2012.4911. PMC 3791056. PMID 23025341.
- Shigenaga, M. K.; Hagen, T. M.; Ames, B. N. (1994). "Oxidative damage and mitochondrial decay in aging". Proceedings of the National Academy of Sciences 91 (23): 10771–8. Bibcode:1994PNAS...9110771S. doi:10.1073/pnas.91.23.10771. JSTOR 2365473. PMC 45108. PMID 7971961.
- Trifunovic, A.; Hansson, A.; Wredenberg, A.; Rovio, A. T.; Dufour, E.; Khvorostov, I.; Spelbrink, J. N.; Wibom, R.; Jacobs, H. T.; Larsson, N.-G. (2005). "Somatic mtDNA mutations cause aging phenotypes without affecting reactive oxygen species production". Proceedings of the National Academy of Sciences 102 (50): 17993–8. Bibcode:2005PNAS..10217993T. doi:10.1073/pnas.0508886102. JSTOR 4152716. PMC 1312403. PMID 16332961.
- Aledo, Juan Carlos; Li, Yang; De Magalhães, João Pedro; Ruíz-Camacho, Manuel; Pérez-Claros, Juan Antonio (2011). "Mitochondrially encoded methionine is inversely related to longevity in mammals". Aging Cell 10 (2): 198–207. doi:10.1111/j.1474-9726.2010.00657.x. PMID 21108730.
- Ferrari, Carlos K. B. (2004). "Functional foods, herbs and nutraceuticals: Towards biochemical mechanisms of healthy aging". Biogerontology 5 (5): 275–89. doi:10.1007/s10522-004-2566-z. PMID 15547316.
- Taylor, Robert W (2005). "Gene therapy for the treatment of mitochondrial DNA disorders". Expert Opinion on Biological Therapy 5 (2): 183–94. doi:10.1517/14712522.214.171.124. PMID 15757380.
- Bjelakovic, Goran; Nikolova, Dimitrinka; Gluud, Christian (2013). "Antioxidant Supplements to Prevent Mortality". JAMA 310 (11): 1178–9. doi:10.1001/jama.2013.277028. PMID 24045742.
- Brown, W. M.; George, M.; Wilson, A. C. (1979). "Rapid evolution of animal mitochondrial DNA". Proceedings of the National Academy of Sciences 76 (4): 1967–71. Bibcode:1979PNAS...76.1967B. doi:10.1073/pnas.76.4.1967. JSTOR 69636. PMC 383514. PMID 109836.
- http://www.promega.ca/~/media/files/resources/profiles%20in%20dna/103/mitochondrial%20dna%20state%20of%20tennessee%20v%20paul%20ware.pdf[full citation needed]
-  Court case name listed in the appeal. Retrieved 17 April 2015.
-  Defense lawyer. Retrieved 17 April 2015.
-  Dna Tests Got Rorrer Life In Jail * Those Results, Plus `Mountain' Of Circumstantial Evidence, Convinced The Jury Of Her Guilt. Retrieved 17 April 2015.
- "Judge allows DNA in Samantha Runnion case," Associated Press, 18 February 2005. Retrieved 4 April 2007.
- "Canine DNA Admitted In California Murder Case," Pit Bulletin Legal News, 5 December 2013. Retrieved 21 January 2014.
- Nass, M. M. K.; Nass, S (1963). "INTRAMITOCHONDRIAL FIBERS WITH DNA CHARACTERISTICS: I. Fixation and Electron Staining Reactions". The Journal of Cell Biology 19 (3): 593–611. doi:10.1083/jcb.19.3.593. PMC 2106331. PMID 14086138.
- Schatz, G.; Haslbrunner, E.; Tuppy, H. (1964). "Deoxyribonucleic acid associated with yeast mitochondria". Biochemical and Biophysical Research Communications 15 (2): 127–32. doi:10.1016/0006-291X(64)90311-0.
- Kumar, Manjeet; Kapil, Aditi; Shanker, Asheesh (2014). "Mito Sat Plant: Mitochondrial microsatellites database of viridiplantae". Mitochondrion 19: 334–7. doi:10.1016/j.mito.2014.02.002. PMID 24561221.
- Damas, J.; Carneiro, J.; Amorim, A.; Pereira, F. (2013). "Mito Break: The mitochondrial DNA breakpoints database". Nucleic Acids Research 42 (Database issue): D1261–8. doi:10.1093/nar/gkt982. PMC 3965124. PMID 24170808.
- Iwasaki, W.; Fukunaga, T.; Isagozawa, R.; Yamada, K.; Maeda, Y.; Satoh, T. P.; Sado, T.; Mabuchi, K.; Takeshima, H.; Miya, M.; Nishida, M. (2013). "Mito Fish and Mito Annotator: A Mitochondrial Genome Database of Fish with an Accurate and Automatic Annotation Pipeline". Molecular Biology and Evolution 30 (11): 2531–40. doi:10.1093/molbev/mst141. PMC 3808866. PMID 23955518.
- Cawthorn, Donna-Mareè; Steinman, Harris Andrew; Corli Witthuhn, R. (2011). "Establishment of a mitochondrial DNA sequence database for the identification of fish species commercially available in South Africa". Molecular Ecology Resources 11 (6): 979–91. doi:10.1111/j.1755-0998.2011.03039.x. PMID 21689383.
- d'Onorio De Meo, P.; d'Antonio, M.; Griggio, F.; Lupi, R.; Borsani, M.; Pavesi, G.; Castrignano, T.; Pesole, G.; Gissi, C. (2011). "Mito Zoa 2.0: A database resource and search tools for comparative and evolutionary analyses of mitochondrial genomes in Metazoa". Nucleic Acids Research 40 (Database issue): D1168–72. doi:10.1093/nar/gkr1144. PMC 3245153. PMID 22123747.
- Gu, Zuguang; Li, Jie; Gao, Song; Gong, Ming; Wang, Junling; Xu, Hua; Zhang, Chenyu; Wang, Jin (2011). "Inter Mito Base: An annotated database and analysis platform of protein-protein interactions for human mitochondria". BMC Genomics 12: 335. doi:10.1186/1471-2164-12-335. PMC 3142533. PMID 21718467.
- Lee, Y. S.; Oh, J.; Kim, Y. U.; Kim, N.; Yang, S.; Hwang, U. W. (2007). "Mitome: Dynamic and interactive database for comparative mitochondrial genomics in metazoan animals". Nucleic Acids Research 36 (Database issue): D938–42. doi:10.1093/nar/gkm763. PMC 2238945. PMID 17940090.
- Catalano, Domenico; Licciulli, Flavio; Turi, Antonio; Grillo, Giorgio; Saccone, Cecilia; d'Elia, Domenica (2006). "Mito Res: A resource of nuclear-encoded mitochondrial genes and their products in Metazoa". BMC Bioinformatics 7: 36. doi:10.1186/1471-2105-7-36. PMC 1395343. PMID 16433928.