Montescudaio is a comune (municipality) in the Province of Pisa in the Italian region Tuscany, located about 70 km southwest of Florence and about 45 km southeast of Pisa.
- Castril, Spain, since 2006
- Eberstadt, Germany, since 1984
- Charlotte Amalie, U.S. Virgin Islands, since 2010
- Christiansted, U.S. Virgin Islands, since 2010
- Frederiksted, U.S. Virgin Islands, since 2010
- ^ All demographics and other statistics: Italian statistical institute Istat.