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Mot or MOT may refer to:
- Marching on Together, the anthem of Leeds United A.F.C.
- Master of Occupational Therapy
- Materials of Trade
- Ministry of Transport
- Ministry of Transport and Road Safety (Israel)
- Mode of transport
- MOT test, British vehicle roadworthiness test, term MOT also used for the certificate granted
- Motorola stock symbol
- IATA code for Minot International Airport
- Minot (Amtrak station), North Dakota, U.S.
- The area comprising three towns in Delaware: Middleton, Odessa and Townsend
- Michigan Opera Theatre
Science and technology
- A measure of sperm motility
- Magneto-optical trap, in physics
- Molecular Orbital Theory, in chemistry
- Management of Technology
- Microwave Oven Transformer
- MOT (gallery), for contemporary art, East London
- MOT (charity), a Norwegian anti-drug and violence organization
- Museum for Old Techniques, Grimbergen, Belgium
- Museum of Tolerance, a multimedia museum in Los Angeles, California
- Mot, a term used for girl, young woman or girlfriend in Ireland, particularly in the Dublin area
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