Mycobacteriophage

From Wikipedia, the free encyclopedia
Jump to: navigation, search

A mycobacteriophage is a member of a group of bacteriophages known to have mycobacteria as host bacterial species. While originally isolated from the bacterial species Mycobacterium smegmatis and Mycobacterium tuberculosis,[1] the causative agent of tuberculosis, more than 4,200 mycobacteriophage have since been isolated from various environmental and clinical sources. Almost 600 have been completely sequenced.[2] Mycobacteriophages have served as examples of viral lysogeny and of the divergent morphology and genetic arrangement characteristic of many phage types.[3]

All mycobacteriophages found thus far have had double-stranded DNA genomes and have been classified by their structure and appearance into siphoviridae or myoviridae.[4]

Discovery[edit]

A bacteriophage was found to infect Mycobacterium smegmatis in 1947 and was the first documented example of a mycobacteriophage. It was found in cultures of the bacteria originally growing in moist compost.[5] The first bacteriophage that infects M. tuberculosis was discovered in 1954.[6]

Genome architecture[edit]

The first sequenced mycobacteriophage genome was that of mycobacteriophage L5 in 1993.[7] In the following years hundreds of additional genomes have been sequenced.[2] Mycobacteriophages have highly mosaic genomes. Their genome sequences show evidence of extensive horizontal genetic transfer, both between phages and between phages and their mycobacterial hosts. Comparisons of these sequences have helped to explain how frequently genetic exchanges of this type may occur in nature, as well as how phages may contribute to bacterial pathogenicity.[8]

A selection of 60 mycobacteriophages were isolated and had their genomes sequenced in 2009. These genome sequences were grouped into clusters by several methods in an effort to determine similarities between the phages and to explore their genetic diversity. More than half of the phage species were originally found in or near Pittsburgh, Pennsylvania, though others were found in other United States locations, India, and Japan. No distinct differences were found in the genomes of mycobacteriophage species from different global origins.[9]

Mycobacteriophage genomes have been found to contain a subset of genes undergoing more rapid genetic flux than other elements of the genomes. These "rapid flux" genes are exchanged between mycobacteriophage more often and are 50 percent shorter in sequence than the average mycobacteriophage gene.[9]

Applications[edit]

Historically, mycobacteriophage have been used to "type" (i.e. "diagnose") mycobacteria, as each phage infects only one or a few bacterial strains.[10] In the 1980s phages were discovered as tools to genetically manipulate their hosts.[11] For instance, phage TM4 was used to construct shuttle phasmids that replicate as large cosmids in Escherichia coli and as phages in mycobacteria.[12] Shuttle phasmids can be manipulated in E. coli us and used to efficiently introduce foreign DNA into mycobacteria.

Phages with mycobacterial hosts may be especially useful for understanding and fighting mycobacterial infections in humans. A system has been developed to use mycobacteriophage carrying a reporter gene to screen strains of M. tuberculosis for antibiotic resistance.[13] In the future, mycobacteriophage could be used to treat infections by phage therapy.[14][15]

References[edit]

  1. ^ MANKIEWICZ, E. (1961). "Mycobacteriophages isolated from Persons with Tuberculous and Non-tuberculous Conditions". Nature 191 (4796): 1416–1417. doi:10.1038/1911416b0. 
  2. ^ a b Mycobacteriophage Database
  3. ^ Hatfull, Graham F. (13 October 2010). "Mycobacteriophages: Genes and Genomes". Annual Review of Microbiology 64 (1): 331–356. doi:10.1146/annurev.micro.112408.134233. 
  4. ^ Pope, Welkin H.; Jacobs-Sera, Deborah, Russell, Daniel A., Peebles, Craig L., Al-Atrache, Zein, Alcoser, Turi A., Alexander, Lisa M., Alfano, Matthew B., Alford, Samantha T., Amy, Nichols E., Anderson, Marie D., Anderson, Alexander G., Ang, Andrew A. S., Ares, Manuel, Barber, Amanda J., Barker, Lucia P., Barrett, Jonathan M., Barshop, William D., Bauerle, Cynthia M., Bayles, Ian M., Belfield, Katherine L., Best, Aaron A., Borjon, Agustin, Bowman, Charles A., Boyer, Christine A., Bradley, Kevin W., Bradley, Victoria A., Broadway, Lauren N., Budwal, Keshav, Busby, Kayla N., Campbell, Ian W., Campbell, Anne M., Carey, Alyssa, Caruso, Steven M., Chew, Rebekah D., Cockburn, Chelsea L., Cohen, Lianne B., Corajod, Jeffrey M., Cresawn, Steven G., Davis, Kimberly R., Deng, Lisa, Denver, Dee R., Dixon, Breyon R., Ekram, Sahrish, Elgin, Sarah C. R., Engelsen, Angela E., English, Belle E. V., Erb, Marcella L., Estrada, Crystal, Filliger, Laura Z., Findley, Ann M., Forbes, Lauren, Forsyth, Mark H., Fox, Tyler M., Fritz, Melissa J., Garcia, Roberto, George, Zindzi D., Georges, Anne E., Gissendanner, Christopher R., Goff, Shannon, Goldstein, Rebecca, Gordon, Kobie C., Green, Russell D., Guerra, Stephanie L., Guiney-Olsen, Krysta R., Guiza, Bridget G., Haghighat, Leila, Hagopian, Garrett V., Harmon, Catherine J., Harmson, Jeremy S., Hartzog, Grant A., Harvey, Samuel E., He, Siping, He, Kevin J., Healy, Kaitlin E., Higinbotham, Ellen R., Hildebrandt, Erin N., Ho, Jason H., Hogan, Gina M., Hohenstein, Victoria G., Holz, Nathan A., Huang, Vincent J., Hufford, Ericka L., Hynes, Peter M., Jackson, Arrykka S., Jansen, Erica C., Jarvik, Jonathan, Jasinto, Paul G., Jordan, Tuajuanda C., Kasza, Tomas, Katelyn, Murray A., Kelsey, Jessica S., Kerrigan, Larisa A., Khaw, Daryl, Kim, Junghee, Knutter, Justin Z., Ko, Ching-Chung, Larkin, Gail V., Laroche, Jennifer R., Latif, Asma, Leuba, Kohana D., Leuba, Sequoia I., Lewis, Lynn O., Loesser-Casey, Kathryn E., Long, Courtney A., Lopez, A. Javier, Lowery, Nicholas, Lu, Tina Q., Mac, Victor, Masters, Isaac R., McCloud, Jazmyn J., McDonough, Molly J., Medenbach, Andrew J., Menon, Anjali, Miller, Rachel, Morgan, Brandon K., Ng, Patrick C., Nguyen, Elvis, Nguyen, Katrina T., Nguyen, Emilie T., Nicholson, Kaylee M., Parnell, Lindsay A., Peirce, Caitlin E., Perz, Allison M., Peterson, Luke J., Pferdehirt, Rachel E., Philip, Seegren V., Pogliano, Kit, Pogliano, Joe, Polley, Tamsen, Puopolo, Erica J., Rabinowitz, Hannah S., Resiss, Michael J., Rhyan, Corwin N., Robinson, Yetta M., Rodriguez, Lauren L., Rose, Andrew C., Rubin, Jeffrey D., Ruby, Jessica A., Saha, Margaret S., Sandoz, James W., Savitskaya, Judith, Schipper, Dale J., Schnitzler, Christine E., Schott, Amanda R., Segal, J. Bradley, Shaffer, Christopher D., Sheldon, Kathryn E., Shepard, Erica M., Shepardson, Jonathan W., Shroff, Madav K., Simmons, Jessica M., Simms, Erika F., Simpson, Brandy M., Sinclair, Kathryn M., Sjoholm, Robert L., Slette, Ingrid J., Spaulding, Blaire C., Straub, Clark L., Stukey, Joseph, Sughrue, Trevor, Tang, Tin-Yun, Tatyana, Lyons M., Taylor, Stephen B., Taylor, Barbara J., Temple, Louise M., Thompson, Jasper V., Tokarz, Michael P., Trapani, Stephanie E., Troum, Alexander P., Tsay, Jonathan, Tubbs, Anthony T., Walton, Jillian M., Wang, Danielle H., Wang, Hannah, Warner, John R., Weisser, Emilie G., Wendler, Samantha C., Weston-Hafer, Kathleen A., Whelan, Hilary M., Williamson, Kurt E., Willis, Angelica N., Wirtshafter, Hannah S., Wong, Theresa W., Wu, Phillip, Yang, Yun jeong, Yee, Brandon C., Zaidins, David A., Zhang, Bo, Zúniga, Melina Y., Hendrix, Roger W., Hatfull, Graham F., Aziz, Ramy (2011). "Expanding the Diversity of Mycobacteriophages: Insights into Genome Architecture and Evolution". In Aziz, Ramy. PLoS ONE 6 (1): e16329. doi:10.1371/journal.pone.0016329. PMC 3029335. PMID 21298013. 
  5. ^ Gardner, Grace M.; Weiser, Russell S. (1947). "A Bacteriophage for Mycobacterium smegmatis". Proceedings of the Society for Experimental Biology and Medicine 66: 205–206. doi:10.3181/00379727-66-16037. 
  6. ^ Froman, S.; Will, D.W., Bogen, E. (1954). "Bacteriophage active against virulent Mycobacterium tuberculosis I. Isolation and activity". Am. J. Publ. Health 44 (10): 1326–1333. doi:10.2105/AJPH.44.10.1326. 
  7. ^ Hatfull, G. F.; Sarkis, G. J. (1993). "DNA sequence, structure and gene expression of mycobacteriophage L5: A phage system for mycobacterial genetics". Molecular microbiology 7 (3): 395–405. PMID 8459766.  edit
  8. ^ Pedulla, Marisa L; Ford, Michael E, Houtz, Jennifer M, Karthikeyan, Tharun, Wadsworth, Curtis, Lewis, John A, Jacobs-Sera, Debbie, Falbo, Jacob, Gross, Joseph, Pannunzio, Nicholas R, Brucker, William, Kumar, Vanaja, Kandasamy, Jayasankar, Keenan, Lauren, Bardarov, Svetsoslav, Kriakov, Jordan, Lawrence, Jeffrey G, Jacobs, William R, Hendrix, Roger W, Hatfull, Graham F (2003). "Origins of Highly Mosaic Mycobacteriophage Genomes". Cell 113 (2): 171–182. doi:10.1016/S0092-8674(03)00233-2. PMID 12705866. 
  9. ^ a b Hatfull, Graham F.; Jacobs-Sera, Deborah, Lawrence, Jeffrey G., Pope, Welkin H., Russell, Daniel A., Ko, Ching-Chung, Weber, Rebecca J., Patel, Manisha C., Germane, Katherine L., Edgar, Robert H. (19 March 2010). "Comparative Genomic Analysis of 60 Mycobacteriophage Genomes: Genome Clustering, Gene Acquisition, and Gene Size". Journal of Molecular Biology 397 (1): 119–143. doi:10.1016/j.jmb.2010.01.011. PMC 2830324. PMID 20064525. 
  10. ^ Jones Jr, W. D. (1975). "Phage typing report of 125 strains of "Mycobacterium tuberculosis"". Annali Sclavo; rivista di microbiologia e di immunologia 17 (4): 599–604. PMID 820285.  edit
  11. ^ Jacobs WR Jr. 2000. Mycobacterium tuberculosis: a once genetically intractable organism. In Molecular Genetics of the Mycobacteria, ed. GF Hatfull, WR Jacobs Jr, pp. 1–16. Washington, DC: ASM Press
  12. ^ Jacobs, W. R.; Tuckman, M.; Bloom, B. R. (1987). "Introduction of foreign DNA into mycobacteria using a shuttle phasmid". Nature 327 (6122): 532–535. doi:10.1038/327532a0. PMID 3473289.  edit
  13. ^ Mulvey, MC; Sacksteder, KA, Einck, L, Nacy, CA (2012). "Generation of a Novel Nucleic Acid-Based Reporter System To Detect Phenotypic Susceptibility to Antibiotics in Mycobacterium tuberculosis". MBio 3 (2): e00312–11. doi:10.1128/mBio.00312-11. PMC 3312217. PMID 22415006. 
  14. ^ Danelishvili, L; Young, LS, Bermudez, LE (Spring 2006). "In vivo efficacy of phage therapy for Mycobacterium avium infection as delivered by a nonvirulent mycobacterium". Microbial drug resistance (Larchmont, N.Y.) 12 (1): 1–6. doi:10.1089/mdr.2006.12.1. PMID 16584300. 
  15. ^ McNerney, R.; Traore, H. (1 August 2005). "Mycobacteriophage and their application to disease control". Journal of Applied Microbiology 99 (2): 223–233. doi:10.1111/j.1365-2672.2005.02596.x. PMID 16033452. 

External links[edit]