The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well-characterized myelin basic proteins. This complex gene structure is conserved among species, suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes.
A targeted immune response to MBP has been researched in lethal rabies infection. The inoculation of MBP generates increases the permeability of the blood–brain barrier (BBB), allowing immune cells to enter the brain, the primary site of rabies virus replication. In a study of mice infected with Silver-haired bat rabies virus (SHBRV), the mortality rate of mice treated with MBP improved 20%-30% over the untreated control group. It is significant to note that healthy uninfected mice treated with MBP showed an increase in mortality rate between 0% and 40%.
A "molecular mimicry" hypothesis of multiple sclerosis has been suggested, in which T cells are, in essence, confusing MBP with human herpesvirus-6. Researchers in the United States created a synthetic peptide with a sequence identical to that of an HHV-6 peptide. They were able to show that T cells were activated by this peptide. These activated T cells also recognized and initiated an immune response against a synthetically created peptide sequence that is identical to part of human MBP. During their research, they found that the levels of these cross-reactive T cells are significantly elevated in multiple sclerosis patients.
^Sakamoto Y, Kitamura K, Yoshimura K, Nishijima T, Uyemura K (March 1987). "Complete amino acid sequence of PO protein in bovine peripheral nerve myelin". J. Biol. Chem.262 (9): 4208–14. PMID2435734.
^Deber CM, Reynolds SJ (April 1991). "Central nervous system myelin: structure, function, and pathology". Clin. Biochem.24 (2): 113–34. PMID1710177.
^Inouye H, Kirschner DA (January 1991). "Folding and function of the myelin proteins from primary sequence data". J. Neurosci. Res.28 (1): 1–17. doi:10.1002/jnr.490280102. PMID1710279.
^Eylar EH, Brostoff S, Hashim G, Caccam J, Burnett P (September 1971). "Basic A1 protein of the myelin membrane. The complete amino acid sequence". J. Biol. Chem.246 (18): 5770–84. PMID5096093.
^Saxe DF, Takahashi N, Hood L, Simon MI (1985). "Localization of the human myelin basic protein gene (MBP) to region 18q22----qter by in situ hybridization". Cytogenet. Cell Genet.39 (4): 246–9. doi:10.1159/000132152. PMID2414074.
^Hoek KS, Schlegel NC, Eichhoff OM, Widmer DS, Praetorius C, Einarsson SO, Valgeirsdottir S, Bergsteinsdottir K, Schepsky A, Dummer R, Steingrimsson E (December 2008). "Novel MITF targets identified using a two-step DNA microarray strategy". Pigment Cell Melanoma Res21 (6): 665–76. doi:10.1111/j.1755-148X.2008.00505.x. PMID19067971.
^Berger T, Rubner P, Schautzer F, Egg R, Ulmer H, Mayringer I, Dilitz E, Deisenhammer F, Reindl M (July 2003). "Antimyelin antibodies as a predictor of clinically definite multiple sclerosis after a first demyelinating event". N. Engl. J. Med.349 (2): 139–45. doi:10.1056/NEJMoa022328. PMID12853586.
Boylan KB, Ayres TM, Popko B, et al. (1990). "Repetitive DNA (TGGA)n 5' to the human myelin basic protein gene: a new form of oligonucleotide repetitive sequence showing length polymorphism.". Genomics6 (1): 16–22. doi:10.1016/0888-7543(90)90443-X. PMID1689270.
Kishimoto A, Nishiyama K, Nakanishi H, et al. (1985). "Studies on the phosphorylation of myelin basic protein by protein kinase C and adenosine 3':5'-monophosphate-dependent protein kinase.". J. Biol. Chem.260 (23): 12492–9. PMID2413024.
Saxe DF, Takahashi N, Hood L, Simon MI (1985). "Localization of the human myelin basic protein gene (MBP) to region 18q22----qter by in situ hybridization.". Cytogenet. Cell Genet.39 (4): 246–9. doi:10.1159/000132152. PMID2414074.
Kamholz J, de Ferra F, Puckett C, Lazzarini R (1986). "Identification of three forms of human myelin basic protein by cDNA cloning.". Proc. Natl. Acad. Sci. U.S.A.83 (13): 4962–6. doi:10.1073/pnas.83.13.4962. PMC323864. PMID2425357.
Scoble HA, Whitaker JN, Biemann K (1986). "Analysis of the primary sequence of human myelin basic protein peptides 1-44 and 90-170 by fast atom bombardment mass spectrometry.". J. Neurochem.47 (2): 614–6. doi:10.1111/j.1471-4159.1986.tb04544.x. PMID2426402.
Roth HJ, Kronquist K, Pretorius PJ, et al. (1986). "Isolation and characterization of a cDNA coding for a novel human 17.3K myelin basic protein (MBP) variant.". J. Neurosci. Res.16 (1): 227–38. doi:10.1002/jnr.490160120. PMID2427738.
Popko B, Puckett C, Lai E, et al. (1987). "Myelin deficient mice: expression of myelin basic protein and generation of mice with varying levels of myelin.". Cell48 (4): 713–21. doi:10.1016/0092-8674(87)90249-2. PMID2434243.
Kamholz J, Spielman R, Gogolin K, et al. (1987). "The human myelin-basic-protein gene: chromosomal localization and RFLP analysis.". Am. J. Hum. Genet.40 (4): 365–73. PMC1684086. PMID2437795.
Roth HJ, Kronquist KE, Kerlero de Rosbo N, et al. (1987). "Evidence for the expression of four myelin basic protein variants in the developing human spinal cord through cDNA cloning.". J. Neurosci. Res.17 (4): 321–8. doi:10.1002/jnr.490170402. PMID2442403.
Shoji S, Ohnishi J, Funakoshi T, et al. (1988). "Phosphorylation sites of bovine brain myelin basic protein phosphorylated with Ca2+-calmodulin-dependent protein kinase from rat brain.". J. Biochem.102 (5): 1113–20. PMID2449425.
Wood DD, Moscarello MA (1989). "The isolation, characterization, and lipid-aggregating properties of a citrulline containing myelin basic protein.". J. Biol. Chem.264 (9): 5121–7. PMID2466844.
Edwards AM, Ross NW, Ulmer JB, Braun PE (1989). "Interaction of myelin basic protein and proteolipid protein.". J. Neurosci. Res.22 (1): 97–102. doi:10.1002/jnr.490220113. PMID2467009.
Streicher R, Stoffel W (1989). "The organization of the human myelin basic protein gene. Comparison with the mouse gene.". Biol. Chem. Hoppe-Seyler370 (5): 503–10. PMID2472816.
Lennon VA, Wilks AV, Carnegie PR (1971). "Immunologic properties of the main encephalitogenic peptide from the basic protein of human myelin.". J. Immunol.105 (5): 1223–30. PMID4099924.
Carnegie PR (1972). "Amino acid sequence of the encephalitogenic basic protein from human myelin.". Biochem. J.123 (1): 57–67. PMC1176899. PMID4108501.
Baldwin GS, Carnegie PR (1971). "Specific enzymic methylation of an arginine in the experimental allergic encephalomyelitis protein from human myelin.". Science171 (3971): 579–81. doi:10.1126/science.171.3971.579. PMID4924231.
Baldwin GS, Carnegie PR (1972). "Isolation and partial characterization of methylated arginines from the encephalitogenic basic protein of myelin.". Biochem. J.123 (1): 69–74. PMC1176900. PMID5128665.
Wood DD, Vella GJ, Moscarello MA (1985). "Interaction between human myelin basic protein and lipophilin.". Neurochem. Res.9 (10): 1523–31. doi:10.1007/BF00964678. PMID6083474.
Gibson BW, Gilliom RD, Whitaker JN, Biemann K (1984). "Amino acid sequence of human myelin basic protein peptide 45-89 as determined by mass spectrometry.". J. Biol. Chem.259 (8): 5028–31. PMID6201481.
Pribyl TM, Campagnoni CW, Kampf K, et al. (1993). "The human myelin basic protein gene is included within a 179-kilobase transcription unit: expression in the immune and central nervous systems.". Proc. Natl. Acad. Sci. U.S.A.90 (22): 10695–9. doi:10.1073/pnas.90.22.10695. PMC47844. PMID7504278.