Malate dehydrogenase (decarboxylating)

From Wikipedia, the free encyclopedia
  (Redirected from NAD-malic enzyme)
Jump to: navigation, search
NAD-malic enzyme
Identifiers
EC number 1.1.1.39
CAS number 9028-46-0
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum
Gene Ontology AmiGO / EGO

Malate dehydrogenase (decarboxylating) (EC 1.1.1.39) or NAD-malic enzyme (NAD-ME) is an enzyme that catalyzes the chemical reaction

(S)-malate + NAD+ \rightleftharpoons pyruvate + CO2 + NADH

Thus, the two substrates of this enzyme are (S)-malate and NAD+, whereas its three products are pyruvate, CO2, and NADH. Malate is oxidized to pyruvate and CO2, and NAD+ is reduced to NADH.

This enzyme belongs to the family of oxidoreductases, to be specific, those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is (S)-malate:NAD+ oxidoreductase (decarboxylating). This enzyme participates in pyruvate metabolism and carbon fixation. NAD-malic enzyme is one of three decarboxylation enzymes used in the inorganic carbon concentrating mechanisms of C4 and CAM plants. The others are NADP-malic enzyme and PEP carboxykinase.[1][2]

Structural studies[edit]

As of late 2007, 7 structures have been solved for this class of enzymes, with PDB accession codes 1DO8, 1EFK, 1EFL, 1PJ2, 1PJ3, 1PJ4, and 1QR6.

References[edit]

  1. ^ Kanai R, Edwards, GE (1999). "3. The Biochemistry of C4 Photosynthesis". In Sage RF, Monson RK. C4 Plant Biology. pp. 43–87. ISBN 0126144400. 
  2. ^ Christopher JT, Holtum JAM (1996). "Patterns of carbon partitioning in leaves of Crassulacean acid metabolism species during deacidification". Plant Physiol. 112 (1): 393–399. PMC 157961. PMID 12226397. 
  • Saz HJ and Hubbard JA (1957). "The oxidative decarboxylation of malate by Ascaris lumbricoides". J. Biol. Chem. 225: 921–933. PMID 13416294.