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NPO may refer to:
- Non-Profit Organization
- National Preservation Office, part of the British Library
- Nauchno-Proizvodstvennoye Obyedineniye (Научно-производственное объединение, Russian for "Scientific and Production Establishment"), title often found in the names of successors to the Experimental Design Bureaux (OKB) of Communist times
- Netherlands Pathfinder Organisation (Nederlandse Padvinders Organisatie), one of the organisations that evolved into Scouting Nederland, the national Scouting organisation
- Netherlands Public Broadcasting (Nederlandse Publieke Omroep), Dutch public radio and television network
- Nil per os, Latin for "nothing by mouth", a medical instruction to withhold oral intake of food and fluids from a patient
- North Pacific Oscillation, an atmospheric teleconnection pattern
- NP optimization problem, optimization problems that are NP-hard
- NPO UK Ltd., a company which had stationery and book shops around Northern Ireland, bought by and rebranded Eason & Son
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