Nattokinase (pronounced nat-oh-KY-nase) is an enzyme (EC 22.214.171.124) extracted and purified from a Japanese food called nattō. Nattō is made from fermented soybeans and has been eaten in Japan for many years. Nattō is produced by fermentation by adding the bacterium Bacillus natto to boiled soybeans. Nattokinase is produced by the bacterium acting on the soybeans. While other soy foods contain enzymes, it is only the nattō preparation that contains the specific nattokinase enzyme.
In spite of its name, nattokinase is not a kinase enzyme, but a serine protease of the subtilisin family. It exhibits a strong fibrinolytic activity. Nattokinase can now be produced by recombinant means and in batch culture, rather than relying on extraction from Nattō.(in India it is marketed by the brand name Orokinase((Nouveau Medicament (P) Limited. Major producer and supplier is Zytex Biotech Private Limited))
Use as an alternative medicine
Nattokinase may be potentially useful as a clot-buster and blood thinner, and is considered by some in the alternative medicine community as a possible adjunctive therapy in cardiovascular disease. There is some evidence for its effectiveness. Possible side effects have been reported. In one case, a patient concurrently taking aspirin and 400 mg of nattokinase daily for seven consecutive days to prevent stroke suffered an acute cerebellar hemorrhage.
A 2009 study showed that nattokinase may be effective in catabolism of toxic amyloid fibrils associated with Alzheimer's Disease, as well as the insulin fibrils associated with diabetes and the prion peptide fibrils associated with prion diseases. The study reported that its effects were similar to those seen when proteinase K was applied in the same manner. The study's authors noted that "[t]his amyloid-degrading ability of nattokinase suggests that it may be useful in the treatment of amyloid-related diseases," particularly as "[p]revious results in rats, dogs and humans have suggested that nattokinase can enter the circulation when taken orally." However, they cautioned that their results were preliminary, and made the following suggestion:"[S]ince natto has been ingested by humans for a long time, it would be worthwhile to carry out an epidemiological study on the rate of occurrence of various amyloid-related diseases in a population regularly consuming natto."
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- Fujita, M.; Nomura, K.; Hong, K.; Ito, Y.; Asada, A.; Nishimuro, S. (1993). "Purification and Characterization of a Strong Fibrinolytic Enzyme (Nattokinase) in the Vegetable Cheese Natto, a Popular Soybean Fermented Food in Japan". Biochemical and Biophysical Research Communications 197 (3): 1340–1347. doi:10.1006/bbrc.1993.2624. PMID 8280151.
- Sumi, H.; Hamada, H.; Tsushima, H.; Mihara, H.; Muraki, H. (1987). "A novel fibrinolytic enzyme (nattokinase) in the vegetable cheese Natto; a typical and popular soybean food in the Japanese diet". Experientia 43 (10): 1110–1111. PMID 3478223.
- Liang, X.; Jia, S.; Sun, Y.; Chen, M.; Chen, X.; Zhong, J.; Huan, L. (2007). "Secretory Expression of Nattokinase from Bacillus subtilis YF38 in Escherichia coli". Molecular Biotechnology 37 (3): 187–194. doi:10.1007/s12033-007-0060-y. PMID 17952663.
- Li, X.; Wang, X.; Xiong, S.; Zhang, J.; Cai, L.; Yang, Y. (2007). "Expression and purification of recombinant nattokinase in Spodoptera frugiperda cells". Biotechnology Letters 29 (10): 1459–1464. doi:10.1007/s10529-007-9426-2. PMID 17581705.
- Cho, Y. H.; Song, J. Y.; Kim, K. M.; Kim, M. K.; Lee, I. Y.; Kim, S. B.; Kim, H. S.; Han, N. S.; Lee, B. H.; Kim, B. S. (2010). "Production of nattokinase by batch and fed-batch culture of Bacillus subtilis". New Biotechnology 27 (4): 341–346. doi:10.1016/j.nbt.2010.06.003. PMID 20541632.
- Kwon, E. Y.; Kim, K. M.; Kim, M. K.; Lee, I. Y.; Kim, B. S. (2011). "Production of nattokinase by high cell density fed-batch culture of Bacillus subtilis". Bioprocess and Biosystems Engineering 34 (7): 789–793. doi:10.1007/s00449-011-0527-x. PMID 21336955.
- Raw Nattokinase
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