|Jmol-3D images||Image 1|
|Molar mass||288.78 g/mol|
| (what is: / ?)
Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa)
|Neutral red (pH indicator)|
|below pH 6.8||above pH 8.0|
Neutral Red (or toluylene red, Basic Red 5, or C.I. 50040) is a eurhodin dye used for staining in histology. It stains lysosomes red. It is used as a general stain in histology, as a counterstain in combination with other dyes, and for many staining methods. Together with Janus Green B, it is used to stain embryonal tissues and supravital staining of blood. Can be used for staining Golgi apparatus in cells and Nissl granules in neurons.
Neutral red can be used as a vital stain. Live cells will incorporate neutral red into their lysosomes. As cells begin to die, their ability to incorporate neutral red diminishes. Thus loss of neutral red uptake corresponds to loss of cell viability.
- Winckler, J. Vital staining of lysosomes and other cell organelles of the rat with neutral Red. Prog. Histochem. Cytochem. 6, 1–89 (1974).
- RepettoG, del Peso A, Zurita JL. Neutral red uptake assay for the estimation of cellviability/cytotoxicity. Nat Protoc. 2008;3(7):1125–1131.doi:10.1038/nprot.2008.75.